FB2024_03 , released June 25, 2024
Allele: Dmel\DgRNAi.UAS
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General Information
Symbol
Dmel\DgRNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0145086
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dsDg
Key Links
Transgenic product class
Nature of the Allele
Transgenic product class
Progenitor genotype
Carried in construct
Cytology
Description

UASt regulatory sequences drives expression of a Dg hairpin loop (that should produce dsRNA when expressed).

Allele components
Component
Use(s)
Encoded product / tool
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing DgdsRNA.UAS under the control of Scer\GAL4Bx-MS1096 affects the formation of the posterior crossvein to a variable degree, from total absence to slight reduction.

Expression of DgdsRNA.Scer\UAS under the control of either Scer\GAL4tub.PU or Scer\GAL4NP4255 results in significantly fewer M-phase neuroblasts than in controls.

Induction of clones expressing DgdsRNA.Scer\UAS under the control of Scer\GAL4tub.PU causes abnormal clustering between clonal and non-clonal cells, resulting in altered 'cobblestone-like' brain organisation.

Expression of DgdsRNA.Scer\UAS under the control of Scer\GAL4GMR.PU does not increase the frequency of premature axon stops in the optic lobe compared to controls.

Expression of DgdsRNA.Scer\UAS under the control of Scer\GAL4tub.PU has no effect on brain lobe morphology.

Flies expressing DgdsRNA.Scer\UAS under the control of Scer\GAL4tub.PU show moderate indirect flight muscle degeneration.

The mean amplitude of the evoked junctional current (EJC) is decreased by approximately 40% at the neuromuscular junction in larvae expressing DgdsRNA.Scer\UAS under the control of Scer\GAL4how-24B compared to in controls. The miniature junctional current (mEJC) amplitude is not decreased in the mutant animals. Quantal content is reduced by approximately 40% in the mutant larvae.

Expression of DgdsRNA.Scer\UAS under the control of Scer\GAL4elav.PU does not significantly affect the mean amplitude of the mEJC or the quantal content at the larval neuromuscular junction.

Expression of DgdsRNA.Scer\UAS under the control of Scer\GAL4tub.PU results in wing vein phenotypes: branches off the posterior crossvein are seen and there is extra material above vein L2.

Third instar larval muscle sarcomeres are significantly smaller in animals expressing DgdsRNA.Scer\UAS under the control of Scer\GAL4tub compared to controls. There is no difference in the overall size of muscles in these animals, and no defects in muscle attachment.

Third instar larval muscle sarcomeres are much more variable in size in animals expressing DgdsRNA.Scer\UAS under the control of Scer\GAL4how-24B compared to controls at 25[o]C. Individual larvae show either small or larger sarcomeres in each muscle, and a combination of both small and large sarcomeres in a single muscle is not seen. There is no difference in the overall size of muscles in these animals, and no defects in muscle attachment.

Third instar larval muscle sarcomeres are larger in animals expressing DgdsRNA.Scer\UAS under the control of Scer\GAL4how-24B compared to controls at 30[o]C. There is no difference in the overall size of muscles in these animals, and no defects in muscle attachment.

There is no effect on third instar larval muscle size or muscle sarcomere size in animals expressing DgdsRNA.Scer\UAS under the control of Scer\GAL469B compared to controls.

Expression of DgdsRNA.Scer\UAS under the control of Scer\GAL4how-24B, Scer\GAL4tub, or Scer\GAL469B does not affect crawling behaviour of third instar larvae.

There is significantly higher muscle membrane resistance in larvae expressing DgdsRNA.Scer\UAS under the control of Scer\GAL4how-24B at either 25[o]C or 30[o]C compared to controls.

Flies expressing DgdsRNA.Scer\UAS under the control of either Scer\GAL4tub or the muscle-specific driver Scer\GAL4how-24B, show comparable climbing ability at the beginning of adult life to control flies. However, the ability of these animals to climb declines significantly faster over time compared to controls. By 12 days (when expression is driven by Scer\GAL4tub) or 20 days (when expression is driven by Scer\GAL4how-24B), age-dependent muscle degeneration is observed including loss of muscle fibre organisation, vacuolisation and the absence of some muscles.

Retinal photoreceptor cells are not elongated in adult eyes expressing DgdsRNA.Scer\UAS under the control of either Scer\GAL4tub.

Expression of DgdsRNA.Scer\UAS in the eye disc (under the control of Scer\GAL4GMR.PU), or in all glial cells (under the control of Scer\GAL4repo.PU), both result in photoreceptor axon targeting defects. Axons stop irregularly, making gaps in the normal termination zone of the lamina plexus, deviating from the path and bundling aberrantly. Expression of DgdsRNA.Scer\UAS in the mesodermal tissue (under the control of Scer\GAL4how-24B) does not effect the axon termination process.

Expression of DgdsRNA.Scer\UAS under the control of Scer\GAL4αTub84B.PL results in a multi-layered follicle epithelium.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

DgRNAi.UAS, Scer\GAL4Tub.PU has visible phenotype, enhanceable by stan[+]/stan192

DgRNAi.UAS, Scer\GAL4Tub.PU has visible phenotype, enhanceable by wg[+]/wgspd-1

DgRNAi.UAS, Scer\GAL4Tub.PU has visible phenotype, enhanceable by grhs2140/grh[+]

DgRNAi.UAS, Scer\GAL4Tub.PU has visible phenotype, enhanceable by Dadj1E4/Dad[+]

DgRNAi.UAS, Scer\GAL4Tub.PU has visible phenotype, enhanceable by msk[+]/msk5

DgRNAi.UAS, Scer\GAL4Tub.PU has visible phenotype, enhanceable by kek1[+]/kek1k07332

DgRNAi.UAS, Scer\GAL4Tub.PU has visible phenotype, enhanceable by aos[+]/aosΔ7

DgRNAi.UAS, Scer\GAL4Tub.PU has visible phenotype, enhanceable by kisk13416/kis[+]

DgRNAi.UAS, Scer\GAL4Tub.PU has visible phenotype, enhanceable by nAChRα6EY13897/nAChRalpha6[+]

DgRNAi.UAS, Scer\GAL4Tub.PU has visible phenotype, enhanceable by POSH[+]/POSHk15815

DgRNAi.UAS, Scer\GAL4Tub.PU has visible phenotype, enhanceable by vimark16722/vimar[+]

DgRNAi.UAS, Scer\GAL4Tub.PU has visible phenotype, enhanceable by Rack1[+]/Rack1EY00128

DgRNAi.UAS, Scer\GAL4Tub.PU has visible phenotype, enhanceable by robo12/robo1[+]

DgRNAi.UAS, Scer\GAL4Tub.PU has visible phenotype, enhanceable by Sdc10608/Sdc[+]

Suppressed by
Statement
Reference

DgRNAi.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by mbl[+]/mblE27

DgRNAi.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by Sema1ak13702/Sema-1a[+]

DgRNAi.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by robo2[+]/robo2lea-2

DgRNAi.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by wun[+]/wunk10201

NOT suppressed by
Statement
Reference
Suppressor of
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Statement
Reference
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Expressing NbrdsRNA.UAS under the control of Scer\GAL4Bx-MS1096 greatly ameliorates posterior crossvein formation defects in flies coexpressing DgdsRNA.UAS.

Expression of DgdsRNA.Scer\UAS under the control of Scer\GAL4GMR.PU partially rescues the premature termination phenotype seen in the optic lobes of Df(2R)KT40 mutant flies.

Expression of DgdsRNA.Scer\UAS under the control of Scer\GAL4tub.PU partially suppresses the 'cobblestone-like' phenotype seen in Df(2R)KT40 mutant brains.

Df(2R)KT40 does not suppress the reduction in M-phase neuroblasts seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of chifEY05746 enhances the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of FhosA055 enhances the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of FhosEY09842 enhances the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of CG7845EMS-Mod4 enhances the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of mblE27 enhances the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of dyscc03838 enhances the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of uif2B7 enhances the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of vimark16722 enhances the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of vimar09 enhances the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of captE636 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of captE593 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of chifBG02820a does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of chifA507 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of Lis-1G10.14 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of Lis-1k11702 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of Lis-1k13209 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of Nrkk14301 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of nAChRα6EY13897 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of nAChRα6KG05852 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of Camn339 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of Rack1EY00128 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of Rack1EE does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of Rack11.8 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of uif1 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of gcmKG01117 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of gcmrA87 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of Dmnk16109 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of POSHk15815 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of delKG10262 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of del3 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of Fkbp1400734 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of grhIM does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of robo12 suppresses the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of SP2353MB00605 does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

Co-expression of DgdsRNA.Scer\UAS and DysdsRNA.C.Scer\UAS under the control of Scer\GAL4tub.PU results in a thickened L3 vein, a phenotype not seen when either transgene is expressed alone.

One copy of DysEMS-ModE10 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of nAChRα6EY13897 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of Sema-2aunspecified enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of robo12 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of Sdc10608 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of stan192 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of wgspd-1 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of grhs2140 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of DlEMS-Mod130 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of DlEMS-Mod140 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of Dadj1E4 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of dppKG08191 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of mskEMS-Mod90 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of msk5 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of kek1k07332 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of aosΔ7 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of kisk13416 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of gcmKG01117 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of POSHk15815 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of vimark16722 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of delKG10262 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of Rack1EY00128 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of chifBG02820a enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of CG7277unspecified enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of Pgkunspecified enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of CG6700unspecified enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of mblE27 suppresses the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of Sema-1ak13702 suppresses the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of robo2lea-2 suppresses the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

One copy of wunk10201 suppresses the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

The oocyte polarity defects seen when Ras85DΔC40B mutant clones are made in the posterior follicle cell layer are partially rescued by expression of DgdsRNA.Scer\UAS in the clone using Scer\GAL4Act and the 'MARCM' technique.

The polarity defects of posterior follicle cells (PFCs) seen in Ras85DΔC40B mutant PFC clones are partially rescued by expression of DgdsRNA.Scer\UAS in the clone using Scer\GAL4Act and the 'MARCM' technique.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
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Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
DgRNAi.UAS
DgdsRNA.Scer\UAS
DgdsRNA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (12)