FB2024_03 , released June 25, 2024
Allele: Dmel\robo2lea-2
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General Information
Symbol
Dmel\robo2lea-2
Species
D. melanogaster
Name
FlyBase ID
FBal0011613
Feature type
allele
Associated gene
Dmel\robo2
Associated Insertion(s)
Carried in Construct
Also Known As
lea2
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
Progenitor genotype
Cytology
Description
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      robo2lea-2 homozygous embryonic heart cardioblasts show a rounded morphology, show significant decreases in migration velocity, as well as in filopodial and lamellopodial extensions and activities, as compared to controls.

      (Raza and Jacobs, 2016)

      learobo2-8/lea2 stage 13 embryos have gonads with unfused somatic gonadal precursor (SGP) clusters. By stage 15, SGP clusters fuse but gonads then fail to compact properly. Mutants also show germ cell ensheathment defects.

      (Weyers et al., 2011)

      lea2 embryos show defective heart development. Heart defects include contralaterally placed cardial cell nuclei, missing cardial cells from rows of migrating cardial cells and a low level of delays in cell migration.

      (MacMullin and Jacobs, 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT suppressed by
      NOT Enhancer of
      Suppressor of
      Statement
      Reference

      robo2[+]/robo2lea-2 is a suppressor of visible phenotype of DgRNAi.UAS, Scer\GAL4Tub.PU

      (Kucherenko et al., 2008)

      robo2[+]/robo2lea-2 is a suppressor of visible phenotype of DysRNAi.C.UAS, Scer\GAL4Tub.PU

      (Kucherenko et al., 2008)
      Other
      Phenotype Manifest In
      Suppressed by
      NOT suppressed by
      Enhancer of
      Statement
      Reference

      lea[+]/robo2lea-2 is an enhancer of eye phenotype of Scer\GAL4GMR.PF, fruNP0021

      (Goto et al., 2011)

      lea[+]/robo2lea-2 is an enhancer of heart primordium phenotype of sli2

      (MacMullin and Jacobs, 2006)
      NOT Enhancer of
      Suppressor of
      Statement
      Reference

      robo2[+]/robo2lea-2 is a suppressor of posterior crossvein phenotype of DgRNAi.UAS, Scer\GAL4Tub.PU

      (Kucherenko et al., 2008)

      robo2[+]/robo2lea-2 is a suppressor of posterior crossvein phenotype of DysRNAi.C.UAS, Scer\GAL4Tub.PU

      (Kucherenko et al., 2008)
      Other
      Statement
      Reference

      DysRNAi.C.UAS, robo2[+]/robo2lea-2 has wing vein | ectopic phenotype

      (Kucherenko et al., 2008)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      robo11, robo2lea-2 double homozygous embryonic heart cardioblast show similar cell rounding, slow migration and decreased filopodial and lamellopodial activities) to those observed in robo11 single homozygotes; these defects are associated with gaps in the leading edge (and lost adhesions with ipsilateral partners), cell clumping, improper linear alignment of the cardioblasts, and heart lumen formation defects, as compared to controls. The decreased lamellopodial activity, but not the decreased filopodial activity, slow migration, or heart lumen formation, are only partially suppressed by the expression of robo1Scer\UAS.cKa under the control of Scer\GAL4Mef2.247.

      (Raza and Jacobs, 2016)

      A lea2 background enhances the rough eye phenotype related to fru isoform B, found upon expression of fruNP0021 under the control of Scer\GAL4GMR.PF.

      (Goto et al., 2011)

      One copy of robo2lea-2 weakly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.C.Scer\UAS is expressed under the control of Scer\GAL4tub.PU but produces extra wing vein material.

      One copy of robo2lea-2 suppresses the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

      (Kucherenko et al., 2008)

      lea2/+, sli2/+ embryos produce a mild heart development phenotype, with some rows of migrating cardial cells missing cells and slight delays in cardial cell migration.

      lea2/+, scb01288/+ embryos show a heart formation phenotype, including the appearance of contralaterally placed cardial cell nuclei.

      (MacMullin and Jacobs, 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      References (12)