FlyBase Allele Report: Dmel\grh[s2140]

General Information

Symbol

Dmel\grh^s2140

Species

D. melanogaster

Name

FlyBase ID

FBal0009305

Feature type

allele

Associated gene

Dmel\grh

Associated Insertion(s)

P{lacW}grh^s2140

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{lacW}grh^s2140

Cytology

Description

Insertion in the first intron of grh.

(Hemphala et al., 2003)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity | somatic clone

(Gambis et al., 2011)

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Phenotype Manifest In

ganglionic tracheal branch primordium (with Df(2R)Pcl7B)

(Hemphala et al., 2003)

ommatidium | somatic clone

(Gambis et al., 2011)

presumptive embryonic/larval tracheal system (with Df(2R)Pcl7B)

(Hemphala et al., 2003)

tracheal dorsal trunk primordium (with Df(2R)Pcl7B)

(Hemphala et al., 2003)

tracheal lateral trunk primordium (with Df(2R)Pcl7B)

(Hemphala et al., 2003)

tracheal transverse connective primordium (with Df(2R)Pcl7B)

(Hemphala et al., 2003)

Detailed Description

Statement

Reference

Homozygous clones in the retina have symmetric ommatidia. In mosaic ommatidia adopting the wrong chiral form, the cell in the R4 position is always mutant, whereas the cell in the R3 position is equally likely to be mutant or wild type.

(Gambis et al., 2011)

Early stage 16 grh^s2140/Df(2R)Pcl7B embryos have more convoluted and elongated dorsal trunks (DTs) than wild-type embryos. This phenotype subsequently becomes more exaggerated, and by the end of stage 16 is also seen in additional branches of the tracheal system, including the lateral trunk, transverse connectives and ganglionic branches. Terminal branching is normal in these embryos, but at the end of embryogenesis the terminal branches become convoluted and often make loops.

(Hemphala et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

grh^s2140/grh[+] is an enhancer of visible phenotype of Dg^RNAi.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Suppressor of

Statement

Reference

grh^s2140/grh[+] is a suppressor of visible phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2008)

grh^s2140/grh[+] is a suppressor of visible phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Other

Statement

Reference

Dys^E6, grh^s2140/grh[+] has visible phenotype

(Kucherenko et al., 2008)

Phenotype Manifest In

Enhancer of

Statement

Reference

grh^s2140/grh[+] is an enhancer of posterior crossvein phenotype of Dg^RNAi.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Suppressor of

Statement

Reference

grh^s2140/grh[+] is a suppressor of posterior crossvein phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2008)

grh^s2140/grh[+] is a suppressor of posterior crossvein phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

grh^s2140/grh^s2140 is a suppressor of ganglionic tracheal branch primordium phenotype of bnl⁰⁰⁸⁵⁷

(Hemphala et al., 2003)

Other

Statement

Reference

Dys^E6, grh^s2140/grh[+] has posterior crossvein phenotype

(Kucherenko et al., 2008)

Dys^RNAi.NH2.UAS, grh^s2140/grh[+] has wing vein | ectopic phenotype

(Kucherenko et al., 2008)

Dys^RNAi.C.UAS, grh^s2140/grh[+] has wing vein | ectopic phenotype

(Kucherenko et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of grh^s2140 strongly suppresses the detached posterior crossvein phenotype seen when Dys^{dsRNA.NH2.Scer\UAS} is expressed under the control of Scer\GAL4^Act.PU but produces extra wing vein material.

One copy of grh^s2140 strongly suppresses the detached posterior crossvein phenotype seen when Dys^{dsRNA.C.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU but produces extra wing vein material.

One copy of grh^s2140 in a Dys^E6/+ background results in posterior crossvein defects.

One copy of grh^s2140 enhances the posterior crossvein phenotype seen when Dg^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU.

(Kucherenko et al., 2008)

The bnl⁰⁰⁸⁵⁷/+ tracheal branch outgrowth defects are partially suppressed by grh^s2140/grh^s2140; 78% of the tracheal ganglionic branches reach the central nervous system in the double mutant embryos.

(Hemphala et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

grh⁰⁶⁸⁵⁰

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type