FlyBase Allele Report: Dmel\POSH[k15815]

General Information

Symbol

Dmel\POSH^k15815

Species

D. melanogaster

Name

FlyBase ID

FBal0064077

Feature type

allele

Associated gene

Dmel\POSH

Associated Insertion(s)

P{lacW}POSH^k15815

Carried in Construct

Also Known As

l(2)k15815

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Zhang et al., 2010)

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}POSH^k15815

Cytology

Description

P{lacW} insertion in the first exon.

(Schnorr et al., 2001)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:17,569,047..17,569,047 [-]

(Zhang et al., 2010, Schnorr et al., 2001, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adherens junction

(Zhang et al., 2010)

embryonic dorsal epidermis | dorsal closure stage

(Zhang et al., 2010)

embryonic dorsal epidermis | dorsal closure stage (with Df(2R)BSC45)

(Zhang et al., 2010)

embryonic dorsal epidermis | dorsal closure stage (with POSH^p92)

(Zhang et al., 2010)

embryonic dorsal epidermis | dorsal closure stage (with POSH^p327)

(Zhang et al., 2010)

embryonic head epidermis

(Zhang et al., 2010)

embryonic head epidermis (with Df(2R)BSC45)

(Zhang et al., 2010)

embryonic head epidermis (with POSH^p92)

(Zhang et al., 2010)

embryonic head epidermis (with POSH^p327)

(Zhang et al., 2010)

embryonic leading edge cell

(Zhang et al., 2010)

filamentous actin

(Zhang et al., 2010)

germ band

(Zhang et al., 2010)

germ band (with Df(2R)BSC45)

(Zhang et al., 2010)

germ band (with POSH^p92)

(Zhang et al., 2010)

germ band (with POSH^p327)

(Zhang et al., 2010)

Detailed Description

Statement

Reference

POSH^k15815/+ mutant flies exhibit temperature-induced mobility defects.

(Kucherenko et al., 2011)

Approximately 20% of POSH^p327/Df(2R)BSC45 embryos show a dorsal hole in the epidermis indicating the disruption of dorsal closure.

Approximately 6% of POSH^k15815/POSH^p327 embryos show a dorsal hole in the epidermis indicating the disruption of dorsal closure.

Approximately 11% of the homozygous POSH^k15815 embryos show a dorsal hole in the epidermis indicating the disruption of dorsal closure.

Approximately 6% of POSH^k15815/POSH^p92 embryos show a dorsal hole in the epidermis indicating the disruption of dorsal closure.

Approximately 6% of the homozygous POSH^k15815 embryos display head involution defects.

Approximately 8% of the homozygous POSH^k15815 embryos display germ-band retraction defects.

None of the homozygous POSH^k15815 embryos hatch to the first instar larval stage.

Approximately 9% of the POSH^k15815/POSH^p92 embryos display head involution defects.

Approximately 10% of the POSH^k15815/POSH^p92 embryos display germ-band retraction defects.

Only 12.5% of the POSH^k15815/POSH^p92 embryos hatch to the first instar larval stage.

Approximately 3% of the POSH^k15815/POSH^p327 embryos display head involution defects.

Approximately 2% of the POSH^k15815/POSH^p327 embryos display germ-band retraction defects.

Only 24.5% of the POSH^k15815/POSH^p327 embryos hatch to the first instar larval stage.

Approximately 5% of the POSH^k15815/Df(2R)BSC45 embryos display head involution defects.

Approximately 7% of the POSH^k15815/Df(2R)BSC45 embryos display germ-band retraction defects.

Only about 9% of the POSH^k15815/Df(2R)BSC45 embryos hatch to the first instar larval stage.

Homozygous mutants of POSH^k15815 that can not finish dorsal closure display abnormal F-actin distribution along the boundaries of leading edge cells of the migrating embryonic epidermis and the amnioserosa. Adherens junctions also show abnormal patterns in POSH^k15815 mutants suggesting aberrant cell adhesions.

(Zhang et al., 2010)

POSH^k15815 mutant clones are arrested prior to stage 3-4 of oogenesis and lack oocytes.

(Kucherenko et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

POSH[+]/POSH^k15815 is an enhancer of visible phenotype of Dg^RNAi.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

NOT Enhancer of

Statement

Reference

POSH^k15815 is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Ketosugbo et al., 2017)

Suppressor of

Statement

Reference

POSH[+]/POSH^k15815 is a suppressor of visible phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2008)

POSH[+]/POSH^k15815 is a suppressor of visible phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

NOT Suppressor of

Statement

Reference

POSH^k15815 is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Ketosugbo et al., 2017)

Phenotype Manifest In

Enhancer of

Statement

Reference

POSH[+]/POSH^k15815 is an enhancer of posterior crossvein phenotype of Dg^RNAi.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

NOT Enhancer of

Statement

Reference

POSH^k15815 is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Ketosugbo et al., 2017)

POSH[+]/POSH^k15815 is a non-enhancer of indirect flight muscle cell phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

POSH[+]/POSH^k15815 is a non-enhancer of indirect flight muscle cell phenotype of Dg^RNAi.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

Suppressor of

Statement

Reference

POSH[+]/POSH^k15815 is a suppressor of posterior crossvein phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2008)

POSH[+]/POSH^k15815 is a suppressor of posterior crossvein phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

NOT Suppressor of

Statement

Reference

POSH^k15815 is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Ketosugbo et al., 2017)

Other

Statement

Reference

Dys^RNAi.C.UAS, POSH[+]/POSH^k15815 has wing vein | ectopic phenotype

(Kucherenko et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

POSH Dys double heterozygous flies (POSH^k15815/Df(3R)Exel6184) do not exhibit indirect flight muscle degeneration.

POSH^k15815 Dg^O86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

One copy of POSH^k15815 does not enhance the indirect flight muscle degeneration seen when Dys^{dsRNA.NH2.Scer\UAS} is expressed under the control of Scer\GAL4^Act.PU.

One copy of POSH^k15815 does not enhance the indirect flight muscle degeneration seen when Dg^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU.

POSH^k15815 Dg³²³ double heterozygous flies do not exhibit indirect flight muscle degeneration.

(Kucherenko et al., 2011)

One copy of POSH^k15815 weakly suppresses the detached posterior crossvein phenotype seen when Dys^{dsRNA.NH2.Scer\UAS} is expressed under the control of Scer\GAL4^Act.PU.

One copy of POSH^k15815 moderately suppresses the detached posterior crossvein phenotype seen when Dys^{dsRNA.C.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU but produces extra wing vein material.

One copy of POSH^k15815 enhances the posterior crossvein phenotype seen when Dg^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU.

(Kucherenko et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

POSH^k11507

(Schnorr et al., 2001)

POSH^k06202

(BDGP Project Members, 1994-1999)

POSH^k15512

(BDGP Project Members, 1994-1999)

Not rescued by

POSH^k15815 is not rescued by Scer\GAL4^unspecified/POSH^UAS.cSa

(Zhang et al., 2010)

Comments

Over-expression of POSH^Scer\UAS.cSa under the control of Scer\GAL4^pnr-MD237 fails to rescue the lethality and morphological defects in POSH^k15815 and POSH^p92 mutants.

(Zhang et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

11146

y¹ w^67c23; P{lacW}MESR4^k15815 P{lacW}POSH^k15815/CyO

Kyoto

111348

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}POSH^k15815 /CyO y⁺

Notes on Origin