FlyBase Allele Report: Dmel\POSH[UAS.cSa]

General Information

Symbol

Dmel\POSH^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Seong

FlyBase ID

FBal0138436

Feature type

allele

Associated gene

Dmel\POSH

Associated Insertion(s)

Carried in Construct

P{UAS-POSH.S}

Also Known As

UAS-POSH

Key Links

Transgenic product class

cDNA

(Seong et al., 2001)

wild_type

(Seong et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Seong et al., 2001)

wild_type

(Seong et al., 2001)

Mutagen

in vitro construct

(Seong et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-POSH.S}

Cytology

Description

UASt sequences drive expression of a POSH cDNA.

(Seong et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

POSH

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of second instar larval stage, with Scer\GAL4^Act5C.PU

(Zhang et al., 2010)

lethal - all die before end of second instar larval stage, with Scer\GAL4^Tub.PU

(Zhang et al., 2010)

long lived, with Scer\GAL4^Appl.G1a

(Seong et al., 2001)

majority die during embryonic stage, with Scer\GAL4^pnr-MD237

(Zhang et al., 2010)

visible, with Scer\GAL4^GMR.PF

(Tsuda et al., 2006)

visible, with Scer\GAL4^GMR.PU

(Zhang et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Overexpression of POSH^Scer\UAS.cSa in the eye disc (under the control of Scer\GAL4^GMR.PF) produces a rough eye phenotype.

(Tsuda et al., 2010)

Animals over-expressing POSH^Scer\UAS.cSa under the control of Scer\GAL4^pnr-MD237 die as embryos with severe dorsal closure defects.

Approximately 18% of the embryos over-expressing POSH^Scer\UAS.cSa under the control of Scer\GAL4^pnr-MD237 display germ-ban retraction defects.

Approximately 15% of the embryos over-expressing POSH^Scer\UAS.cSa under the control of Scer\GAL4^Act5C.PU display germ-ban retraction defects.

Approximately 72% of the embryos over-expressing POSH^Scer\UAS.cSa under the control of Scer\GAL4^Act5C.PU display dorsal closure defects.

Over-expression of POSH^Scer\UAS.cSa in the eye using Scer\GAL4^GMR.PU results in a rough-eye phenotype.

Over-expression of POSH^Scer\UAS.cSa in the whole body driven by Scer\GAL4^Act5C.PU or Scer\GAL4^tub.PU causes early stage lethality. Most of the animals die at the embryonic stage and only a few can survive to the first or second instar larval stage.

Adherens junctions along the boundaries of leading edge cells of the migrating embryonic epidermis and the amnioserosa in embryos overexpressing POSH^Scer\UAS.cSa driven by Scer\GAL4^Act5C.PU show abnormal patterns.

(Zhang et al., 2010)

Expression of POSH^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PF induces a rough-eye phenotype.

(Tsuda et al., 2006)

When POSH^Scer\UAS.cSa is driven by Scer\GAL4^Appl.G1a, an extended longevity phenotype is seen. Mean longevity at 25^oC is about 49.4 days (compared to 43.4 days for controls).

(Seong et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

POSH^UAS.cSa, Ras85D^V12.UAS, Scer\GAL4^Act5C.PI has neoplasia | somatic clone | larval stage phenotype, suppressible by wts^UAS.cYa, Scer\GAL4^Act5C.PI

(Ma et al., 2020)

POSH^UAS.cSa, Scer\GAL4^pnr-MD237 has lethal - all die during embryonic stage phenotype, suppressible | partially by Tak1[+]/Tak1^unspecified

(Zhang et al., 2010)

NOT suppressed by

Statement

Reference

POSH^UAS.cSa, Scer\GAL4^pnr-MD237 has lethal - all die during embryonic stage phenotype, non-suppressible by bsk²/bsk[+]

(Zhang et al., 2010)

POSH^UAS.cSa, Scer\GAL4^pnr-MD237 has lethal - all die during embryonic stage phenotype, non-suppressible by hep[+]/hep^r75

(Zhang et al., 2010)

POSH^UAS.cSa, Scer\GAL4^GMR.PU has visible phenotype, non-suppressible by egr^UAS.cIa, Scer\GAL4^GMR.PU

(Zhang et al., 2010)

Enhancer of

Statement

Reference

POSH^UAS.cSa, Scer\GAL4^Act5C.PI is an enhancer of neoplasia | larval stage | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI

(Ma et al., 2020)

POSH^UAS.cSa, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of ALiX^UAS.cTa, Scer\GAL4^GMR.PF

(Tsuda et al., 2006)

POSH^UAS.cSa, Scer\GAL4^GMR.PF is an enhancer of melanotic necrosis phenotype of ALiX^UAS.cTa, Scer\GAL4^GMR.PF

(Tsuda et al., 2006)

Suppressor of

Statement

Reference

POSH^UAS.cSa, Scer\GAL4^GMR.PF is a suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa

(Tsuda et al., 2010)

POSH^UAS.cSa, Scer\GAL4^GMR.PU is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PU, egr^UAS.cIa

(Zhang et al., 2010)

Other

Statement

Reference

Alg-2^UAS.cTa, POSH^UAS.cSa, Scer\GAL4^GMR.PF has visible phenotype

(Tsuda et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

POSH^UAS.cSa, Scer\GAL4^GMR.PF, egr^UAS.cMa has eye phenotype, enhanceable by Akt^UAS.cSa, Scer\GAL4^GMR.PF

(Tsuda et al., 2010)

Suppressed by

Statement

Reference

POSH^UAS.cSa, Ras85D^V12.UAS, Scer\GAL4^Act5C.PI has eye disc | somatic clone phenotype, suppressible by wts^UAS.cYa, Scer\GAL4^Act5C.PI

(Ma et al., 2020)

POSH^UAS.cSa, Scer\GAL4^GMR.PF, egr^UAS.cMa has eye phenotype, suppressible by POSH⁷⁴

(Tsuda et al., 2010)

POSH^UAS.cSa, Scer\GAL4^GMR.PF, egr^UAS.cMa has eye phenotype, suppressible by Akt1[+]/Akt¹

(Tsuda et al., 2010)

POSH^UAS.cSa, Scer\GAL4^GMR.PF, egr^UAS.cMa has eye phenotype, suppressible by Pten^UAS.cHa, Scer\GAL4^GMR.PF

(Tsuda et al., 2010)

POSH^UAS.cSa, Scer\GAL4^pnr-MD237 has embryonic dorsal epidermis | dorsal closure stage phenotype, suppressible by Tak1[+]/Tak1^unspecified

(Zhang et al., 2010)

NOT suppressed by

Statement

Reference

POSH^UAS.cSa, Scer\GAL4^GMR.PU has eye phenotype, non-suppressible by egr^UAS.cIa, Scer\GAL4^GMR.PU

(Zhang et al., 2010)

Enhancer of

Statement

Reference

POSH^UAS.cSa, Scer\GAL4^Act5C.PI is an enhancer of eye disc | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI

(Ma et al., 2020)

POSH^UAS.cSa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa

(Tsuda et al., 2010)

POSH^UAS.cSa/Akt^UAS.cSa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa

(Tsuda et al., 2010)

POSH^UAS.cSa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of ALiX^UAS.cTa, Scer\GAL4^GMR.PF

(Tsuda et al., 2006)

Suppressor of

Statement

Reference

Rel^GS20403/POSH^UAS.cSa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, egr^UAS.cMa

(Tsuda et al., 2010)

POSH^UAS.cSa, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PU, egr^UAS.cIa

(Zhang et al., 2010)

Other

Statement

Reference

POSH^UAS.cSa, Scer\GAL4^pnr-MD237, Tak1[+]/Tak1^unspecified has adult external thorax phenotype

(Zhang et al., 2010)

Alg-2^UAS.cTa, POSH^UAS.cSa, Scer\GAL4^GMR.PF has eye phenotype

(Tsuda et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Flies overexpressing POSH^Scer\UAS.cSa and egr^Scer\UAS.cMa in the developing eye (both under the control of Scer\GAL4^GMR.PF) exhibit larger eyes than those expressing egr^Scer\UAS.cMa alone. In addition, these double mutants exhibit a highly deformed eye structure. These phenotypes are reverted with a single copy of POSH⁷⁴ (that reduces the level of POSH).

Eye size in POSH^Scer\UAS.cSa egr^Scer\UAS.cMa (Scer\GAL4^GMR.PF overexpression mutants is reduced in an Akt1¹ heterozygous background.

Eye size in POSH^Scer\UAS.cSa egr^Scer\UAS.cMa (Scer\GAL4^GMR.PF overexpression mutants is reduced upon co-expression of Pten^Scer\UAS.cHa.

The eye size increase observed in POSH^Scer\UAS.cSa egr^Scer\UAS.cMa (Scer\GAL4^GMR.PF overexpression mutants is enhanced upon co-expression of Akt1^Scer\UAS.cSa.

Co-expression of Rel^GS20403 together with POSH^Scer\UAS.cSa and egr^Scer\UAS.cMa in the developing eye under the control of Scer\GAL4^GMR.PF results in very large eyes (i.e. enhances the phenotype).

(Tsuda et al., 2010)

hep^r75/+ is unable to suppress the Scer\GAL4^pnr-MD237>POSH^Scer\UAS.cSa lethality phenotype.

bsk²/+ is unable to suppress the Scer\GAL4^pnr-MD237>POSH^Scer\UAS.cSa lethality phenotype.

Tak1^unspecified/+ partially suppresses the lethal dorsal open phenotype of Scer\GAL4^pnr-MD237>POSH^Scer\UAS.cSa. Escapees survive to adulthood with thorax defects.

Overexpression of POSH^Scer\UAS.cSa can suppress the small-eye phenotype of Scer\GAL4^GMR.PU>egr^Scer\UAS.cIa, although a rough-eye phenotype is still observed.

(Zhang et al., 2010)

Co-expression of POSH^Scer\UAS.cSa and Alg-2^Scer\UAS.cTa under the control of Scer\GAL4^GMR.PF results in small eyes.

Co-expression of POSH^Scer\UAS.cSa and ALiX^Scer\UAS.cTa under the control of Scer\GAL4^GMR.PF enhances the eye phenotype induced by Scer\GAL4^GMR.PF-driven expression of ALiX^Scer\UAS.cTa alone, increasing the level of melanotic pigments but not affecting eye size.

(Tsuda et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Scer\GAL4^unspecified/POSH^UAS.cSa fails to rescue POSH^k15815

(Zhang et al., 2010)

Scer\GAL4^unspecified/POSH^UAS.cSa fails to rescue POSH^p92

(Zhang et al., 2010)

Comments

Over-expression of POSH^Scer\UAS.cSa under the control of Scer\GAL4^pnr-MD237 fails to rescue the lethality and morphological defects in POSH^k15815 and POSH^p92 mutants.

(Zhang et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58990

y¹ w^*; P{UAS-POSH.S}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

POSH^Scer\UAS.cSa

POSH^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Seong

(Seong et al., 2001)

Secondary FlyBase IDs

References (8)

Report Sections

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