FlyBase Allele Report: Dmel\chif[BG02820a]

General Information

Symbol

Dmel\chif^BG02820a

Species

D. melanogaster

Name

FlyBase ID

FBal0130461

Feature type

allele

Associated gene

Dmel\chif

Associated Insertion(s)

P{GT1}chif^BG02820a

Carried in Construct

Also Known As

chif^BG02820, chif-GAL4, BG02820

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{GT1}chif^BG02820a

Cytology

Description

The P{GT1}chif^BG02820a insertion is within an intron of both chif and of CG42231 (overlapping genes). In addition, Southern blotting using Scer\GAL4 DNA suggests the presence of two additional insertions in the 'BG02820' line: P{GT1}BG02820b and P{GT1}BG02820c.

(Tunstall et al., 2012)

Allele components

Component

Use(s)

Inserted element

P{GT1}

gene trap

polyA trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:16,351,662..16,351,662 [+]2L:16,351,662..16,351,662 [+]

(Chen et al., 2014, Tunstall et al., 2012, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates muscular dystrophy

modeled by Dg^O86

(Marrone et al., 2011)

exacerbates muscular dystrophy

modeled by Dg^O86

(Kucherenko et al., 2011)

modeled by Dg³²³

(Kucherenko et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

FlyBase curator comment: Interactions with Dg[086] and/or Dg[323] were detected in a Dg[086]/+ or Dg[323]/+ background which exhibits no obvious changes in muscle morphology. Nonetheless, these interactions have been captured as 'modifier' ('exacerbates') annotations here to best capture the experimental finding and the authors' intention.

(Kucherenko et al., 2011)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | dominant | temperature conditional

(Kucherenko et al., 2011)

abnormal neuroanatomy | third instar larval stage

(Marrone et al., 2011)

Phenotype Manifest In

axon | third instar larval stage

(Marrone et al., 2011)

indirect flight muscle cell (with chif^EY05746)

(Kucherenko et al., 2011)

lamina plexus | third instar larval stage

(Marrone et al., 2011)

oocyte | somatic clone

(Kucherenko et al., 2008)

Detailed Description

Statement

Reference

chif^EY05746/chif^BG02820a mutant flies show significant indirect flight muscle degeneration compared to controls.

chif^BG02820a/chif^A507 mutant flies show significant indirect flight muscle degeneration compared to controls.

chif^BG02820a/+ mutant flies exhibit temperature-induced mobility defects.

(Kucherenko et al., 2011)

chif^BG02820a homozygous mutant third instar larvae display a significantly increased frequency of axon migration abnormalities in the brain compared to controls, resulting in defects in the lamina plexus.

(Marrone et al., 2011)

chif^BG02820a mutant germline clones show oocyte mislocalisation.

(Kucherenko et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

chif[+]/chif^BG02820a is an enhancer of visible phenotype of Dg^RNAi.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Other

Statement

Reference

Dg^O86, chif[+]/chif^BG02820a has abnormal neuroanatomy | third instar larval stage phenotype

(Marrone et al., 2011)

Phenotype Manifest In

Enhancer of

Statement

Reference

chif[+]/chif^BG02820a is an enhancer of posterior crossvein phenotype of Dg^RNAi.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

NOT Enhancer of

Statement

Reference

chif[+]/chif^BG02820a is a non-enhancer of indirect flight muscle cell phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

chif[+]/chif^BG02820a is a non-enhancer of indirect flight muscle cell phenotype of Dg^RNAi.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

Other

Statement

Reference

Df(3R)Exel6184/+, chif^BG02820a has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg^O86, chif^BG02820a has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg³²³, chif^BG02820a has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg^O86, chif[+]/chif^BG02820a has lamina plexus | third instar larval stage phenotype

(Marrone et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

chif Dys double heterozygous flies (chif^BG02820a/Df(3R)Exel6184) exhibit indirect flight muscle degeneration.

chif^BG02820a Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

One copy of chif^BG02820a does not enhance the indirect flight muscle degeneration seen when Dys^{dsRNA.NH2.Scer\UAS} is expressed under the control of Scer\GAL4^Act.PU.

One copy of chif^BG02820a does not enhance the indirect flight muscle degeneration seen when Dg^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU.

chif^BG02820a Dg³²³ double heterozygous flies exhibit indirect flight muscle degeneration.

(Kucherenko et al., 2011)

Combination of Dg^O86 in heterozygous state with a single copy of chif^BG02820a results in significantly decreased frequency of lamina plexus defects in the third instar larvae.

(Marrone et al., 2011)

One copy of chif^BG02820a enhances the posterior crossvein phenotype seen when Dg^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU.

(Kucherenko et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

13134