FB2024_03 , released June 25, 2024
Insertion: Dmel\P{GT1}chifBG02820a
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General Information
Symbol
Dmel\P{GT1}chifBG02820a
Species
D. melanogaster
Name
FlyBase ID
FBti0180787
Feature type
transposable_element_insertion_site
Also Known As
chif-GAL4, BG02820
Description
Inserted Element
P{GT1}
LINE ID

BG02820

Affected gene(s)
chif, Hyls1, Scer\GAL4
Viability / fertility
Associated allele(s)
chifBG02820a, Hyls1BG02820a, Scer\GAL4BG02820a
Stock availability
3 publicly available
Genomic Location
Chromosomal Location
2L ( 35F12 )
Sequence Location
2L:16,351,662..16,351,662 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{GT1}.BG
Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GT1}. The P{GT1} construct is described as a dual-tagged gene trap (FBrf0132344):it carries (1) w+mGT1 mini-white marker, designed to confer a pigmented eye phenotype only if the construct is inserted within a host gene, and (2) a promoter-less Scer\GAL4 gene which is expressed if it is produced as a fusion with the mRNA produced by the host gene. The construct also carries a Tn\neoR selectable marker (FBrf0132344).

(Bellen et al., 2004)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:16,351,662..16,351,662 [+]
(Gene Disruption Project members, 2001-)
2L:16,351,662..16,351,662 [+]
(Gene Disruption Project members, 2001-)
Orientation
Cytological location (computed by FlyBase)
35F12 (inferred by FlyBase from sequence location)
Cytological location (reported)
35F10 (reported as inferred from sequence location)
(Gene Disruption Project members, 2001-)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Inserted Element
Construct
P{GT1}
(Magwire et al., 2010)
Size (Kb)

8.447

(Lukacsovich and Asztalos, 1999.7.15)
Component Allele(s)
w+mGT
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
spermatogenesis & adult stage | male
spermatocyte
(Bunt et al., 2012)
testis sheath muscle cell
(Bunt et al., 2012)
adult stage | male & spermatogenesis
cyst cell of testis
(Bunt et al., 2012)
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
chif
(Bunt et al., 2012)
Reporter construct used in assay
Data on Genetic Line
Line ID

BG02820

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (3)
External Crossreferences and Linkouts ( 1 )
Linkouts
GDP-BG
  • BG02820a
Comments
Comments

The P{GT1}chifBG02820a insertion is within an intron of both chif and of CG42231 (overlapping genes). In addition, Southern blotting using Scer\GAL4 DNA suggests the presence of two additional insertions in the 'BG02820' line: P{GT1}BG02820b and P{GT1}BG02820c.

(Tunstall et al., 2012)

Location 2L:16347591-16347592 confirmed by FlyBase alignment of dbGSS accession BH615037 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)
Synonyms and Secondary IDs (7)
Reported As
Symbol Synonym
BG02820
(Chen et al., 2014, Tunstall et al., 2012, Gene Disruption Project members, 2001-, FlyBase, 1992-)
P{GT1}CG42231BG02820b
P{GT1}chifBG02820
(FlyBase, 2005, FlyBase, 1992-)
P{GT1}chifBG02820a
chif-GAL4
(Misra et al., 2017)
chif-Gal4
(Kunduri et al., 2022)
chifGAL4
(Bunt et al., 2012)
Name Synonyms
Secondary FlyBase IDs
  • FBti0021329
  • FBti0150319
References (14)