A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GT1}. The P{GT1} construct is described as a dual-tagged gene trap (FBrf0132344):it carries (1) w+mGT1 mini-white marker, designed to confer a pigmented eye phenotype only if the construct is inserted within a host gene, and (2) a promoter-less Scer\GAL4 gene which is expressed if it is produced as a fusion with the mRNA produced by the host gene. The construct also carries a Tn\neoR selectable marker (FBrf0132344).
Mobilization of the P-element occurred in animals carrying P{GT1} on a second chromosome CyO balancer chromosome and P{Δ2-3}99B, a stable source of transposase, on a marked third chromosome. The screen was carried out in isogenized backgrounds; 6 different isogenized backgrounds were used. Genomic locations of new insertions were determined by assessment of flanking sequence by high-throughput methods and balanced stocks were created.
Markers in stocks (as originally submitted to the BDSC):w1118.
2869 lines were generated; flanking genomic sequences were obtained by sequencing of inverse PCR products, and were mapped by alignment to the genomic sequence. 482 lines were selected for inclusion in the BDGP Gene Disruption Project 2004 collection.
Because of their uniform genetic background, lines in this dataset are useful for studies of quantitative traits.
