FlyBase Allele Report: Dmel\dysc[c03838]

General Information

Symbol

Dmel\dysc^c03838

Species

D. melanogaster

Name

FlyBase ID

FBal0160893

Feature type

allele

Associated gene

Dmel\dysc

Associated Insertion(s)

PBac{PB}dysc^c03838

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

piggyBac activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Jepson et al., 2012)

Mutagen

piggyBac activity

(Bloomington Drosophila Stock Center, 2004.11.6)

Progenitor genotype

Associated Insertion(s)

PBac{PB}dysc^c03838

Cytology

Description

dysc^c03838 renders expression of all dysc isoforms undetectable.

(Jepson et al., 2012)

Allele components

Component

Use(s)

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:13,886,309..13,886,309 [+]

(Bloomington Drosophila Stock Center, 2004.11.6)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates muscular dystrophy

modeled by Dg³²³

(Kucherenko et al., 2011)

modeled by Dg^O86

(Kucherenko et al., 2011)

modeled by Dg^RNAi.UAS

(Kucherenko et al., 2011)

modeled by Dys^RNAi.NH2.UAS

(Kucherenko et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

FlyBase curator comment: Interactions with Dg[086] and/or Dg[323] were detected in a Dg[086]/+ or Dg[323]/+ background which exhibits no obvious changes in muscle morphology. Nonetheless, these interactions have been captured as 'modifier' ('exacerbates') annotations here to best capture the experimental finding and the authors' intention.

(Kucherenko et al., 2011)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

NMJ bouton

(Jepson et al., 2014)

NMJ bouton (with dysc^s168)

(Jepson et al., 2014)

type Ib terminal bouton

(Jepson et al., 2014)

type Ib terminal bouton (with dysc^s168)

(Jepson et al., 2014)

type Is terminal bouton

(Jepson et al., 2014)

type Is terminal bouton (with dysc^s168)

(Jepson et al., 2014)

Detailed Description

Statement

Reference

dysc^c03838/dysc^c03838 and dysc^s168/dysc^c03838 third instar larvae show a significant reduction in bouton number (both type 1b and type 1s boutons) and a significant increase in the size of type 1s boutons at the neuromuscular junction.

Homozygous larvae show a significant increase in the rate of body wall peristalsis compared to controls.

Mutant larvae show a significant increase in evoked excitatory junctional current (EJC) amplitude and width compared to controls. There is a significant increase in spontaneous miniature EJC frequency compared to controls.

(Jepson et al., 2014)

Most dysc^c03838 homozygotes and dysc^c03838/Df(3L)BSC614 animals display arrhythmic locomotor patterns in constant dark conditions.

In contrast to wild type flies, dysc^c03838 homozygotes do not exhibit anticipation of lights-on in 12 h light:dark conditions, while anticipation of lights-off is maintained. Overall daytime and nighttime activity is greater in these mutants compared to wild type in these conditions.

dysc^s168/dysc^c03838 animals display arrhythmic locomotor patterns in constant dark conditions.

dysc^c03838/dysc^c05107 animals display arrhythmic locomotor patterns in constant dark conditions.

(Jepson et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

dysc^c03838 has abnormal neurophysiology | third instar larval stage phenotype, non-enhanceable by slo^MB11481

(Jepson et al., 2014)

NOT suppressed by

Statement

Reference

dysc^c03838 has abnormal neurophysiology | third instar larval stage phenotype, non-suppressible by slo^MB11481

(Jepson et al., 2014)

NOT Enhancer of

Statement

Reference

dysc^c03838 is a non-enhancer of abnormal neurophysiology | third instar larval stage phenotype of slo^MB11481

(Jepson et al., 2014)

NOT Suppressor of

Statement

Reference

dysc^c03838 is a non-suppressor of abnormal neurophysiology | third instar larval stage phenotype of slo^MB11481

(Jepson et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

dysc^c03838 has NMJ bouton phenotype, suppressible by slo^MB11481

(Jepson et al., 2014)

Enhancer of

Statement

Reference

dysc[+]/dysc^c03838 is an enhancer of indirect flight muscle cell phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

dysc[+]/dysc^c03838 is an enhancer of indirect flight muscle cell phenotype of Dg^RNAi.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

Other

Statement

Reference

Df(3R)Exel6184/+, dysc^c03838 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg^O86, dysc^c03838 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg^RNAi.UAS, Scer\GAL4^Tub.PU, dysc^c03838 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg³²³, dysc^c03838 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Bouton size at the neuromuscular junction in dysc^c03838 slo^MB11481 double mutant larvae is significantly increased compared to wild type, but is not significantly different from that seen in dysc^c03838 single mutants. The reduction in bouton number at the neuromuscular junction seen in dysc^c03838 larvae is suppressed in dysc^c03838 slo^MB11481 double mutant larvae.

Evoked excitatory junctional currents in dysc^c03838 slo^MB11481 double mutant larvae have the same phenotypes as in either single mutant. Miniature EJC frequency in dysc^c03838 slo^MB11481 double mutant larvae is not significantly different from that in either single mutant.

(Jepson et al., 2014)

dysc Dys double heterozygous flies (dysc^c03838/Df(3R)Exel6184) exhibit indirect flight muscle degeneration.

dysc^c03838 Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

One copy of dysc^c03838 enhances the indirect flight muscle degeneration seen when Dys^{dsRNA.NH2.Scer\UAS} is expressed under the control of Scer\GAL4^Act.PU.

One copy of dysc^c03838 enhances the indirect flight muscle degeneration seen when Dg^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU.

dysc^c03838 Dg³²³ double heterozygous flies exhibit indirect flight muscle degeneration.

(Kucherenko et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

dysc^c03838 is rescued by dysc^UAS.long/Scer\GAL4^elav.PU

(Jepson et al., 2014)

dysc^s168/dysc^c03838 is rescued by dysc^UAS.long/Scer\GAL4^elav.PU

(Jepson et al., 2012)

dysc^s168/dysc^c03838 is rescued by dysc^UAS.long/Scer\GAL4^dysc.PJ

(Jepson et al., 2012)

dysc^s168/dysc^c03838 is rescued by dysc^UAS.long/Scer\GAL4^C164

(Jepson et al., 2012)

Not rescued by

dysc^s168/dysc^c03838 is not rescued by dysc^UAS.long/Scer\GAL4^P2.4.Pdf

(Jepson et al., 2012)

dysc^s168/dysc^c03838 is not rescued by dysc^UAS.long/Scer\GAL4^tim.PE

(Jepson et al., 2012)

dysc^s168/dysc^c03838 is not rescued by dysc^UAS.long/Scer\GAL4^ey-OK107

(Jepson et al., 2012)

dysc^s168/dysc^c03838 is not rescued by dysc^UAS.long/Scer\GAL4^Tab2-201Y

(Jepson et al., 2012)

dysc^s168/dysc^c03838 is not rescued by dysc^UAS.long/Scer\GAL4^c819

(Jepson et al., 2012)

dysc^s168/dysc^c03838 is not rescued by dysc^UAS.long/Scer\GAL4^c107

(Jepson et al., 2012)

dysc^s168/dysc^c03838 is not rescued by dysc^UAS.long/Scer\GAL4^c061

(Jepson et al., 2012)

dysc^s168/dysc^c03838 is not rescued by dysc^UAS.long/Scer\GAL4^Ilp2.PR

(Jepson et al., 2012)

dysc^s168/dysc^c03838 is not rescued by dysc^UAS.long/Scer\GAL4^dimm-929

(Jepson et al., 2012)

dysc^s168/dysc^c03838 is not rescued by dysc^UAS.long/Scer\GAL4^VGlut-OK371

(Jepson et al., 2012)

dysc^s168/dysc^c03838 is not rescued by dysc^UAS.long/Scer\GAL4^how-24B

(Jepson et al., 2012)

dysc^s168/dysc^c03838 is not rescued by dysc^UAS.long/Scer\GAL4^repo.PU

(Jepson et al., 2012)

dysc^s168/dysc^c03838 is not rescued by Scer\GAL4^elav.PU/dysc^UAS.short

(Jepson et al., 2012)

Comments

The increase in evoked excitatory junctional current amplitude seen in dysc^c03838 larvae is rescued by expression of dysc^{Scer\UAS.long} under the control of Scer\GAL4^elav.PU.

(Jepson et al., 2014)

Scer\GAL4^elav.PU-, Scer\GAL4^dysc.PJ-, or Scer\GAL4^C164-mediated expression of dysc^Scer\UAS.g rescues the locomotor rhythm defects of dysc^c03838/dysc^s168 animals.

Scer\GAL4^elav.PU-mediated expression of dysc^Scer\UAS.f does not rescue the locomotor rhythm defects of dysc^c03838/dysc^s168 animals.

(Jepson et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11313

w¹¹¹⁸; PBac{PB}dysc^c03838

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG34400^c03838

(Kucherenko et al., 2011)

CG43749^c03838

CG8760^c03838

dysc^c03838

(Jepson et al., 2014, Jepson et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (5)

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