FlyBase Allele Report: Dmel\Nrk[k14301]

General Information

Symbol

Dmel\Nrk^k14301

Species

D. melanogaster

Name

FlyBase ID

FBal0128148

Feature type

allele

Associated gene

Dmel\Nrk

Associated Insertion(s)

P{lacW}Nrk^k14301

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}Nrk^k14301

Cytology

Description

P{lacW} insertion in exon 1.

(Schnorr et al., 2001)

The P{lacW}Nrk^k14301 insertion maps within both TppII and CG34439 (overlapping genes on opposite strands) by BLAST analysis of the flanking sequence of the insertion. In addition, in FBrf0139680, the P{lacW}Nrk^k14301 insertion is stated to map "within the 5' end of an alternative-splice form of Nrk" (Nrk is located downstream to CG34439, and overlaps it on the same strand).

(FlyBase, 1996-)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:13,158,718..13,158,718 [+]

(Schnorr et al., 2001, FlyBase, 1996-, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates muscular dystrophy

modeled by Dg^O86

(Kucherenko et al., 2011)

modeled by Dg³²³

(Kucherenko et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

FlyBase curator comment: Interactions with Dg[086] and/or Dg[323] were detected in a Dg[086]/+ or Dg[323]/+ background which exhibits no obvious changes in muscle morphology. Nonetheless, these interactions have been captured as 'modifier' ('exacerbates') annotations here to best capture the experimental finding and the authors' intention.

(Kucherenko et al., 2011)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | dominant | temperature conditional

(Kucherenko et al., 2011)

abnormal neuroanatomy | third instar larval stage

(Marrone et al., 2011)

lethal | recessive

(BDGP Project Members, 1994-1999)

Phenotype Manifest In

axon | third instar larval stage

(Marrone et al., 2011)

lamina plexus | third instar larval stage

(Marrone et al., 2011)

Detailed Description

Statement

Reference

Nrk^k14301/+ mutant flies exhibit temperature-induced mobility defects.

(Kucherenko et al., 2011)

Nrk^k14301 homozygous mutant third instar larvae display a significantly increased frequency of axon migration abnormalities in the brain compared to controls, resulting in defects in the lamina plexus.

(Marrone et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Nrk[+]/Nrk^k14301 is a suppressor of visible phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2008)

Nrk[+]/Nrk^k14301 is a suppressor of visible phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Other

Statement

Reference

Df(3R)Exel6184/+, Nrk^k14301 has abnormal neuroanatomy | third instar larval stage phenotype

(Marrone et al., 2011)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Nrk[+]/Nrk^k14301 is a non-enhancer of indirect flight muscle cell phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

Nrk[+]/Nrk^k14301 is a non-enhancer of indirect flight muscle cell phenotype of Dg^RNAi.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

Suppressor of

Statement

Reference

Nrk[+]/Nrk^k14301 is a suppressor of posterior crossvein phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2008)

Nrk[+]/Nrk^k14301 is a suppressor of posterior crossvein phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Other

Statement

Reference

Df(3R)Exel6184/+, Nrk^k14301 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg^O86, Nrk^k14301 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg³²³, Nrk^k14301 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Df(3R)Exel6184/+, Nrk^k14301 has lamina plexus | third instar larval stage phenotype

(Marrone et al., 2011)

Dys^RNAi.C.UAS, Nrk[+]/Nrk^k14301 has wing vein | ectopic phenotype

(Kucherenko et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Nrk Dys double heterozygous flies (Nrk^k14301/Df(3R)Exel6184) exhibit indirect flight muscle degeneration.

Nrk^k14301 Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

One copy of Nrk^k14301 does not enhance the indirect flight muscle degeneration seen when Dys^{dsRNA.NH2.Scer\UAS} is expressed under the control of Scer\GAL4^Act.PU.

One copy of Nrk^k14301 does not enhance the indirect flight muscle degeneration seen when Dg^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU.

Nrk^k14301 Dg³²³ double heterozygous flies exhibit indirect flight muscle degeneration.

(Kucherenko et al., 2011)

Combination of Df(3R)Exel6184 in heterozygous state with a single copy of Nrk^k14301 results in a significantly increased frequency of lamina plexus defects in the third instar larval brain, whereas the rhabdomere length in the adult eye remains unaffected in the double heterozygotes.

Combination of Dg^O86 in heterozygous state with a single copy of Nrk^k14301 does not significantly affect either the frequency of lamina plexus defects in the third instar larvae or the rhabdomere length in the adults.

(Marrone et al., 2011)

One copy of Nrk^k14301 weakly suppresses the detached posterior crossvein phenotype seen when Dys^{dsRNA.NH2.Scer\UAS} is expressed under the control of Scer\GAL4^Act.PU.

One copy of Nrk^k14301 moderately suppresses the detached posterior crossvein phenotype seen when Dys^{dsRNA.C.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU but produces extra wing vein material.

(Kucherenko et al., 2008)

The P{lacW}Nrk^k14301 insertion (which is inserted into 2 overlapping genes; intron 1 of TppII and exon 1 of Nrk) is a moderate dominant enhancer of the Ras85D^ix12a eggshell phenotype; 31-40% of dorsal appendages are wild type.

(Schnorr et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

11122

y¹ w^67c23; P{lacW}Nrk^k14301 ND-MWFE^k14301 TppII^k14301/CyO

Kyoto

111339

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}Nrk^k14301 /CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

This allele was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was discarded from the gene disruption project prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Spradling (spradling@mail1.ciwemb.edu).

Complements: Aats-val⁰³⁵³¹. Complements: Aats-val^k14312. Complements: Aats-val^k14804. Complements: Aats-val^rI255. Complements: Aats-val^rQ802.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Nrk^k14301

(Kucherenko et al., 2011, Marrone et al., 2011, Kucherenko et al., 2008)

l(2)k14301

(Schnorr et al., 2001)

l(2)k14301^k14301

Name Synonyms

Secondary FlyBase IDs

FBal0064109

References (6)

Report Sections

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