FB2024_03 , released June 25, 2024
Insertion: Dmel\P{lacW}Nrkk14301
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General Information
Symbol
Dmel\P{lacW}Nrkk14301
Species
D. melanogaster
Name
FlyBase ID
FBti0006414
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{lacW}
LINE ID

l(2)k14301

Affected gene(s)
Ecol\lacZ, Nrk, TppII
Viability / fertility
Associated allele(s)
Ecol\lacZNrk-k14301, ND-MWFEk14301, Nrkk14301, TppIIk14301
Stock availability
2 publicly available
Genomic Location
Chromosomal Location
2R ( 49F4 )
Sequence Location
2R:13,158,718..13,158,718 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{lacW}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:13,158,718..13,158,718 [+]
(BDGP Project Members, 1994-1999)
Orientation
Cytological location (computed by FlyBase)
49F4 (inferred by FlyBase from sequence location)
Cytological location (reported)
49F7-49F8 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
AQ034035
(FlyBase, 1992-)
Inserted Element
Construct
P{lacW}
Size (Kb)

10.691

Component Allele(s)
w+mC
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID

l(2)k14301

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (2)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Location 2R:8674625-8674626 confirmed by FlyBase alignment of dbGSS accession AQ034035 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.

(FlyBase, 2005)

This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).

(FlyBase, 1992-)
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{lacW}Nrkk14301
(FlyBase, 2005, Schnorr et al., 2001, BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{lacW}l(2)k14301k14301
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
Name Synonyms
Secondary FlyBase IDs
    References (12)