P{lacW} insertion in intron 1.
The P{lacW}Nrkk14301 insertion maps within both TppII and CG34439 (overlapping genes on opposite strands) by BLAST analysis of the flanking sequence of the insertion. In addition, in FBrf0139680, the P{lacW}Nrkk14301 insertion is stated to map "within the 5' end of an alternative-splice form of Nrk" (Nrk is located downstream to CG34439, and overlaps it on the same strand).
The P{lacW}Nrkk14301 insertion (which is inserted into 2 overlapping genes; intron 1 of TppII and exon 1 of Nrk) is a moderate dominant enhancer of the Ras85Dix12a eggshell phenotype; 31-40% of dorsal appendages are wild type.
I. Kiss.
This allele was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was discarded from the gene disruption project prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Spradling (spradling@mail1.ciwemb.edu).
Complements: Aats-val03531. Complements: Aats-valk14312. Complements: Aats-valk14804. Complements: Aats-valrI255. Complements: Aats-valrQ802.