FlyBase Allele Report: Dmel\Lis-1[k13209]

(Kucherenko et al., 2008, Yang et al., 2008, Lei and Warrior, 2000, Spradling et al., 1999, Swan et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates muscular dystrophy

modeled by Dg^O86

(Kucherenko et al., 2011, Marrone et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

FlyBase curator comment: Interactions with Dg[086] and/or Dg[323] were detected in a Dg[086]/+ or Dg[323]/+ background which exhibits no obvious changes in muscle morphology. Nonetheless, these interactions have been captured as 'modifier' ('exacerbates') annotations here to best capture the experimental finding and the authors' intention.

(Kucherenko et al., 2011)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle (with Lis-1^G10.14)

(Siller et al., 2005)

abnormal neuroanatomy | third instar larval stage

(Marrone et al., 2011)

lethal | recessive

(Lei and Warrior, 2000, Spradling et al., 1999, BDGP Project Members, 1994-1999)

lethal - all die before end of pupal stage

(Swan et al., 1999)

some die during pupal stage

(Swan et al., 1999)

Phenotype Manifest In

axon | adult stage

(Vagnoni et al., 2016)

cyst progenitor cell (with Lis-1^k11702)

(Cheng et al., 2011)

dorsal appendage (with Lis-1^k11702)

(Lei and Warrior, 2000)

eye | somatic clone

(Huang et al., 2015)

eye photoreceptor cell | somatic clone

(Huang et al., 2015)

lamina plexus | third instar larval stage

(Marrone et al., 2011)

marginal nerve | adult stage

(Vagnoni et al., 2016)

mitochondrion | adult stage

(Vagnoni et al., 2016)

oocyte | somatic clone

(Kucherenko et al., 2008)

photoreceptor cell & nucleus

(Swan et al., 1999)

retina | somatic clone

(Huang et al., 2015)

rhabdomere of eye photoreceptor cell | somatic clone

(Huang et al., 2015)

spindle (with Lis-1^G10.14)

(Siller et al., 2005)

spindle (with Lis-1^k11702)

(Cheng et al., 2011)

spindle pole centrosome (with Lis-1^G10.14)

(Siller et al., 2005)

Detailed Description

Statement

Reference

Lis-1^k13209/+ results in a significant increase in the number of transported mitochondria in the wing marginal nerve, as compared to controls.

(Vagnoni et al., 2016)

Lis-1^k13209 homozygous mutant clones in the eye have normal morphology at 1 day old and then show retinal degeneration.

(Huang et al., 2015)

Only 15% of testis somatic cyst stem cells show repositioning of the mitotic spindle during anaphase in Lis-1^k11702/Lis-1^k13209 males compared to 72% of cases in heterozygous controls.

(Cheng et al., 2011)

Lis-1^k13209 heterozygous third instar larvae display increased frequency of lamina plexus defects in the brain compared to controls.

(Marrone et al., 2011)

Lis-1^k13209 mutant germline clones show oocyte mislocalisation.

(Kucherenko et al., 2008)

heterozygous mutants do not exhibit a R-cell nuclear migration phenotype.

(Houalla et al., 2005)

In contrast to wild type, centrosome separation is often incomplete at the onset of prometaphase in Lis-1^G10.14/Lis-1^k13209 2nd larval instar brain neuroblasts. Microtubules emanating from both spindle poles often develop a Y-shaped morphology during prometaphase in these mutant cells, presumably because of the close apposition of the centrosomes. These Y-shaped spindles generally "recover" to form seemingly bipolar arrays of kinetochore microtubules by late metaphase. However, microtubule organising centres (MTOCs) occasionally detach from spindles or two MTOCs appear to maintain attachment to the same side of a half-spindle. In a few cases, the mutant neuroblasts contain more than the normal two MTOCs. Curved spindles and unfocused spindle poles are also seen in the mutant neuroblasts at metaphase.

The length of the prometaphase/metaphase interval is dramatically lengthened in Lis-1^G10.14/Lis-1^k13209 neuroblasts compared to wild type (average of 46 minutes 54 seconds +/- 18 minutes 33 seconds compared to 6 minutes 12 seconds +/- 0 minutes 49 seconds respectively). In addition, the mean duration of prometaphase and of metaphase are both individually lengthened in the mutant neuroblasts. The mitotic index and metaphase:anaphase ratio are increased in Lis-1^G10.14/Lis-1^k13209 neuroblasts compared to wild type.

Tension between sister centromeres is reduced in Lis-1^G10.14/Lis-1^k13209 metaphase neuroblasts compared to wild type.

(Siller et al., 2005)

Photoreceptor cell nuclei posterior to the morphogenetic furrow are found more basally in homozygous eye imaginal discs than in wild-type. Photoreceptor cell nuclei are occasionally found in the axons that project basally from these cells.

(Swan et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Lis-1[+]/Lis-1^k13209 is a suppressor of visible phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Other

Statement

Reference

Dg^O86, Lis-1[+]/Lis-1^k13209 has abnormal neuroanatomy | third instar larval stage phenotype

(Marrone et al., 2011)

Phenotype Manifest In

Enhancer of

Statement

Reference

Lis-1^k13209 is an enhancer of photoreceptor cell | precursor & nucleus phenotype of Scer\GAL4^unspecified, msn^DN.UAS

(Houalla et al., 2005)

NOT Enhancer of

Statement

Reference

Lis-1[+]/Lis-1^k13209 is a non-enhancer of indirect flight muscle cell phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

Lis-1[+]/Lis-1^k13209 is a non-enhancer of indirect flight muscle cell phenotype of Dg^RNAi.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

Suppressor of

Statement

Reference

Lis-1[+]/Lis-1^k13209 is a suppressor of posterior crossvein phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Other

Statement

Reference

Df(3R)Exel6184/+, Lis-1^k13209 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg[+]/Dg^O86, Lis-1^k13209 has indirect flight muscle cell phenotype

(Kucherenko et al., 2011)

Dg^O86, Lis-1[+]/Lis-1^k13209 has lamina plexus | third instar larval stage phenotype

(Marrone et al., 2011)

Dys^RNAi.C.UAS, Lis-1[+]/Lis-1^k13209 has wing vein | ectopic phenotype

(Kucherenko et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Lis-1 Dys double heterozygous flies (Lis-1^k13209/Df(3R)Exel6184) exhibit indirect flight muscle degeneration.

Lis-1^k13209 Dg^O86 double heterozygous flies exhibit indirect flight muscle degeneration.

One copy of Lis-1^k13209 does not enhance the indirect flight muscle degeneration seen when Dys^{dsRNA.NH2.Scer\UAS} is expressed under the control of Scer\GAL4^Act.PU.

One copy of Lis-1^k13209 does not enhance the indirect flight muscle degeneration seen when Dg^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU.

Lis-1^k13209 Dg³²³ double heterozygous flies do not exhibit indirect flight muscle degeneration.

(Kucherenko et al., 2011)

The increased frequency of lamina plexus defects characteristic for Lis-1^k13209 heterozygote third instar larvae is not strongly affected by combination with a single copy of either Df(3R)Exel6184 or Dg^O86, the rhabdomere length in the adult eye of the double heterozygotes is not significantly different from the controls.

(Marrone et al., 2011)

One copy of Lis-1^k13209 moderately suppresses the detached posterior crossvein phenotype seen when Dys^{dsRNA.C.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU but produces extra wing vein material.

(Kucherenko et al., 2008)

21% of animals expressing msn^DN.Scer\UAS in the developing eye, combined with Lis-1^k13209/+ exhibit a R-cell nuclear migration phenotype.

(Houalla et al., 2005)

The dramatically increased length of the prometaphase/metaphase interval and increase in both mitotic index and metaphase:anaphase ration that is seen in Lis-1^G10.14/Lis-1^k13209 neuroblasts is not seen if they are also homozygous for rod^H4.8, although the centrosome and spindle assembly defects still remain.

(Siller et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Lis-1^E415

(Swan et al., 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

11072

y¹ w^67c23; P{lacW}Lis-1^k13209/CyO

Kyoto

111319

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}Lis-1^k13209 /CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Excision of the P{lacW} element restores viability.

(Lei and Warrior, 2000)

Complements: l(2)k11702^k11702. Complements: Khc^k13219. Complements: Khc^k13314.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

DLis-1^l(2)k13209

(Houalla et al., 2005)

DLis¹³²⁰⁹

(Lei and Warrior, 2000)

Lis-1^k13209

(Huang et al., 2015, Kucherenko et al., 2008, Yang et al., 2008)

Lis1^k13209

(Vagnoni et al., 2016, Marrone et al., 2011, Siller et al., 2005)

Paf-AHβ^k13209

l(2)k13209

(Spradling et al., 1999)

l(2)k13209^k13209

(BDGP Project Members, 1994-1999)

lis-1^k13209

(Kucherenko et al., 2011)

lis1^K13209

(Beaven et al., 2015)

lis1^k13209

(Cheng et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (14)

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