FlyBase Allele Report: Dmel\Lis-1[E415]

General Information

Symbol

Dmel\Lis-1^E415

Species

D. melanogaster

Name

FlyBase ID

FBal0095665

Feature type

allele

Associated gene

Dmel\Lis-1

Associated Insertion(s)

P{lacW}Lis-1^E415

Carried in Construct

Also Known As

lis1^E415

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Swan et al., 1999)

Mutagen

P-element activity

(Swan et al., 1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}Lis-1^E415

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile (with Df(2R)Jp8)

(Swan et al., 1999)

female sterile | recessive

(Swan et al., 1999)

Phenotype Manifest In

axon | adult stage

(Vagnoni et al., 2016)

axon | adult stage (with Lis-1^k11702)

(Vagnoni et al., 2016)

egg chamber (with Df(2R)Jp8)

(Swan et al., 1999)

marginal nerve | adult stage

(Vagnoni et al., 2016)

marginal nerve | adult stage (with Lis-1^k11702)

(Vagnoni et al., 2016)

microtubule

(Swan et al., 1999)

microtubule organizing center (with Df(2R)Jp8)

(Swan et al., 1999)

mitochondrion | adult stage

(Vagnoni et al., 2016)

mitochondrion | adult stage (with Lis-1^k11702)

(Vagnoni et al., 2016)

nurse cell | increased number (with Df(2R)Jp8)

(Swan et al., 1999)

oocyte

(Swan et al., 1999)

oocyte (with Df(2R)Jp8)

(Swan et al., 1999)

oocyte nucleus

(Swan et al., 1999)

Detailed Description

Statement

Reference

Lis-1^E415/+ results in a significant increase in the number of transported mitochondria in the wing marginal nerve, as compared to controls.

Lis-1^E415/Lis-1^k11702 results in a significant increase in the number of transported mitochondria in the wing marginal nerve in both anterograde and retrograde directions, and a small but significant increase in anterograde run velocity of mitochondria, as measured at several stages of adult life, but there is no significant difference in the total number of mitochondria, mitochondrial transport lengths or retrograde velocity, no significant difference in the proportion of motile dense-core vesicles, compared to controls.

(Vagnoni et al., 2016)

Centrosome positioning and microtubule organisation are not perturbed in cycle 14 blastoderm embryos derived from Lis-1^E415/Lis-1^k11702 females.

(Dix et al., 2013)

Lis-1^E415 interacts with BicD mutations throughout oogenesis.

(Swan et al., 1999)

50% of Lis-1^E415/Df(2R)Jp8 egg chambers contain 16 polyploid nuclei, indicating that each cell has adopted the nurse cell fate. In 20% of egg chambers an oocyte is detectable, but is often mispositioned. The remaining 30% of egg chambers show a later defect in cyst encapsulation. A microtubule organising centre, which normally develops in the oocyte as early as region 2 of the germarium, is usually not seen in Lis-1^E415/Df(2R)Jp8 egg chambers. Lis-1^E415/Df(2R)Jp8 egg chambers rarely contain fewer than 16 cells and appear to develop a normal fusome that is correctly associated with the mitotic spindle. The oocyte is greatly reduced in size in homozygous stage 8 egg chambers. Later stage egg chambers are almost wild-type in size. The oocyte nucleus is positioned incorrectly in 50% of homozygous stage 9 and 10 egg chambers. Some alterations in microtubule organisation are seen after stage 7 in homozygous egg chambers, the microtubules often appear less focused than normal.

(Swan et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Lis-1^E415 has female sterile | recessive phenotype, suppressible by Dhc64C^6-6/Dhc64C[+]

(Swan et al., 1999)

Other

Statement

Reference

Dhc64C^6-6, Lis-1^E415 has fertile phenotype

(Swan et al., 1999)

DCTN1-p150^Gl-1, Lis-1^E415 has lethal phenotype

(Swan et al., 1999)

Dhc64C^6-6/Dhc64C[+], Lis-1^E415 has fertile phenotype

(Swan et al., 1999)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Lis-1^E415 has phenotype, non-enhanceable by Dhc64C^6-12

(Swan et al., 1999)

Suppressed by

Statement

Reference

Lis-1^E415 has oocyte nucleus phenotype, suppressible by Dhc64C^6-6/Dhc64C[+]

(Swan et al., 1999)

Lis-1^E415 has oocyte phenotype, suppressible by Dhc64C^6-6/Dhc64C[+]

(Swan et al., 1999)

NOT suppressed by

Statement

Reference

Lis-1^E415 has phenotype, non-suppressible by Dhc64C^6-12

(Swan et al., 1999)

Enhancer of

Statement

Reference

Lis-1[+]/Lis-1^E415, Scer\GAL4^{VP16.mat.αTub67C} is an enhancer of egg phenotype of Hsap\DCTN2^UAS.cJa, Scer\GAL4^{VP16.mat.αTub67C}

(Januschke et al., 2002)

Lis-1[+]/Lis-1^E415, Scer\GAL4^{VP16.mat.αTub67C} is an enhancer of dorsal appendage phenotype of Hsap\DCTN2^UAS.cJa, Scer\GAL4^{VP16.mat.αTub67C}

(Januschke et al., 2002)

Lis-1[+]/Lis-1^E415 is an enhancer of phenotype of BicD^r

(Swan et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Dhc64C^6-6 is a strong dominant suppressor of the Lis-1^E415 homozygous phenotype, resulting in fertility, proper oocyte nucleus positioning and near normal oocyte growth.

(Swan et al., 1999)

Xenogenetic Interactions

Statement

Reference

The proportion of eggs laid by Hsap\DCTN2^Scer\UAS.cJa; Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} flies with severe dorsal-ventral patterning defects is enhanced by heterozygosity for Lis-1^E415/+.

(Januschke et al., 2002)

Complementation and Rescue Data

Fails to complement

Lis-1^k13209

(Swan et al., 1999)

Rescued by

Lis-1[+]/Lis-1^E415 is rescued by Lis-1^αTub.PD

(Vagnoni et al., 2016)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

99014

w^*; P{lacW}Lis-1^E415/CyO

Notes on Origin

Discoverer

Comments

Excision of the P-element can restore fertility.

(Swan et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

DLis-1^E415

(Januschke et al., 2002)

E415

(Swan et al., 1999, Swan et al., 1999)

Lis-1^E415

Lis1^E415

(Vagnoni et al., 2016, Huynh and St. Johnston, 2004)

lis1^E415

(Dix et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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