FlyBase Allele Report: Dmel\Dhc64C[6-6]

General Information

Symbol

Dmel\Dhc64C^6-6

Species

D. melanogaster

Name

FlyBase ID

FBal0048196

Feature type

allele

Associated gene

Dmel\Dhc64C

Associated Insertion(s)

Carried in Construct

Also Known As

dhc^6-6, Dhc6-6

Key Links

Allele class

hypomorphic allele - genetic evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Abdu et al., 2006)

loss of function allele

(Gepner et al., 1996)

Mutagen

ethyl methanesulfonate

(Gepner et al., 1996)

Progenitor genotype

Cytology

Description

Genomic Southern blotting revealed no polymorphism between the mutant and wild type chromosome.

(Gepner et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

centrosome | maternal effect

(Robinson et al., 1999)

dendrite | pupal stage (with Df(3L)BSC436)

(Lin et al., 2015)

dendrite | pupal stage (with Dhc64C^6-10)

(Lin et al., 2015)

egg

(McGrail and Hays, 1997)

embryo | blastoderm stage | maternal effect

(Robinson et al., 1999)

embryonic/first instar larval cuticle (with Dhc64C^6-8)

(Harris and Peifer, 2005)

germline cyst | germline clone (with Dhc64C^6-12)

(Cox and Spradling, 2006)

Golgi apparatus | embryonic cycle 14 (with Dhc64C^6-8)

(Papoulas et al., 2005)

mitochondrial cloud | germline clone (with Dhc64C^6-12)

(Cox and Spradling, 2006)

mitotic cell cycle | maternal effect

(Robinson et al., 1999)

mitotic domain 1 | embryonic cycle 14 (with Dhc64C^6-8)

(Papoulas et al., 2005)

oocyte (with Dhc64C^6-12)

(Palacios and St. Johnston, 2002, Pare and Suter, 2000)

ovary (with Dhc64C^6-12)

(Pare and Suter, 2000)

primordial germ cell (with Dhc64C^6-10)

(Lerit and Gavis, 2011)

spindle | maternal effect

(Robinson et al., 1999)

Detailed Description

Statement

Reference

Dhc64C^6-6/Dhc64C^6-10 or Dhc64C^6-6/Df(3L)BSC436 pupae (16h after puparium formation) have dendrite pruning defects. Dendrites develop normally in sensory neurons (C4da) of Dhc64C^6-6/Dhc64C^6-10 or Dhc64C^6-6/Df(3L)BSC436 larvae.

(Lin et al., 2015)

Dhc64C^6-6/Dhc64C^6-10 embryos have a reduced number of pole cells compared to controls.

(Lerit and Gavis, 2011)

Cytoplasmic streaming occurs normally in the oocytes of Dhc64C^6-6/Dhc64C^6-12 mutants.

(Mische et al., 2007)

Dhc64C^6-6/Dhc64C^6-12 mutant clonal prefollicular cysts show premature mitochondrial accumulation at the middle of the fusome compared to wild-type cysts. The Balbiani body is reduced in the oocytes that develop from these clones.

(Cox and Spradling, 2006)

Dhc64C^6-6/Dhc64C^6-8 trans-heterozygous mutants are adult viable.

Dhc64C^6-6/Dhc64C^6-8 mutants (m/z mutants) produce large sheets of embryonic cuticle, indicative of relatively normal epithelial polarity.

(Harris and Peifer, 2005)

Embryos derived from Dhc64C^6-6/Dhc64C^6-8 females that develop to nuclear cycle 14 fail to undergo normal furrow formation that is seen between adjacent nuclei in wild-type embryos at this stage. These embryos also accumulate fewer Golgi bodies in the apical cytoplasm compared to wild-type embryos at this stage, despite the presence of intact microtubules.

(Papoulas et al., 2005)

Ooplasmic streaming still occurs in stage 9 Dhc64C^6-6/Dhc64C^6-12 oocytes but it is significantly slower than normal.

(Palacios and St. Johnston, 2002)

Ovaries from Dhc64C^6-6/Dhc64C^6-12 females show a range of phenotypes, including the failure of the oocyte to differentiate.

(Pare and Suter, 2000)

Dhc64C^6-6/Dhc64C^6-8 adults are recovered in equal proportion to nonmutant siblings, indicating that this heteroallelic combination is not less viable than wild type. 94% of embryos derived from Dhc64C^6-6/Dhc64C^6-8 females mated to wild-type males fail to survive beyond the end of embryogenesis. Most of these embryos fail to cellularise properly and cannot complete gastrulation. Approximately 5.7% of the remaining embryos survive through hatching, with 1.4% of these embryos failing to complete larval development. The approximately 3% of embryos that complete larval development survive to adulthood. Two predominant mitotic defects, free centrosomes and multipolar spindle arrays, are found in syncytial blastoderm embryos derived from Dhc64C^6-6/Dhc64C^6-8 females mated to wild-type males that are arrested early in embryogenesis. Free centrosomes are seen either singly or in numbers. The free centrosomes can arise during both early and late nuclear cycles by different pathways that are independent of cell cycle stage. First, centrosomes are seen departing the nuclear envelope during prophase. The detachment of centrosomes from bipolar and multipolar spindles is also seen. Spindles are seen lacking one or both centrosomes at their poles. The mean distance between centrosomes and the associated spindle poles is 1.8μm, significantly higher than wild type. Centrosome migration is abnormal in these embryos. Spindle configurations are often excessively curved and the normally uniform spacing between spindles within the syncytium is disrupted. Multipolar spindle arrays are seen, which most commonly arise from fusion of a number of neighbouring spindles. Spindle-associated or single free centrosomes are also capable of inducing ectopic spindle poles on adjacent mitotic arrays. Abnormal spindles in which an apparently normal half-spindle containing a single centrosome, spindle pole and chromatin is flanked by an abnormally blunt-ended pole lacking a detectable centrosome are also seen. The mitotic defects are detected during very early nuclear cycles. The syncytial nuclear divisions of embryos derived from Dhc64C^6-6/Dhc64C^6-8 females mated to wild-type males show poor synchrony. Dhc64C^6-6/Df(3L)10^H hemizygous larval neuroblasts frequently show a reduced affinity of centrosomes for spindle poles. Spindle microtubule bundles are often disrupted and curved.

(Robinson et al., 1999)

Dhc64C^6-6/Dhc64C^6-12 transheterozygous ovaries produce mature eggs, however, the eggs are fragile and show variable defects in size, shape and number and orientation of the chorionic appendages.

(McGrail and Hays, 1997)

Lethality acts during larval, pupal or pharate adult phase. Low levels (3-12%) of embryonic lethality are observed.

(Gepner et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Dhc64C^6-6/Df(3L)BSC436 has abnormal neuroanatomy | pupal stage phenotype, non-suppressible by spn-F^8D.UAS.EGFP/Scer\GAL4^ppk.PG

(Lin et al., 2015)

Enhancer of

Statement

Reference

Dhc64C^6-6 is an enhancer of visible | dominant phenotype of Egfr^E1

(Iyadurai et al., 2008)

Dhc64C^6-6/Dhc64C^6-8 is an enhancer of lethal | embryonic stage phenotype of baz⁴

(Harris and Peifer, 2005)

Dhc64C^6-6/Dhc64C^6-8 is an enhancer of abnormal cell polarity phenotype of baz⁴

(Harris and Peifer, 2005)

Suppressor of

Statement

Reference

Dhc64C^6-6/Dhc64C[+] is a suppressor of female sterile | recessive phenotype of Lis-1^E415

(Swan et al., 1999)

Dhc64C^6-6/Dhc64C[+] is a suppressor of visible | dominant phenotype of DCTN1-p150^Gl-1

(McGrail et al., 1995)

Other

Statement

Reference

Dhc64C^6-6, Lis-1^E415 has fertile phenotype

(Swan et al., 1999)

Dhc64C^6-6/Dhc64C[+], Lis-1^E415 has fertile phenotype

(Swan et al., 1999)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Dhc64C^6-6/Df(3L)BSC436 has dendrite | pupal stage phenotype, non-suppressible by spn-F^8D.UAS.EGFP/Scer\GAL4^ppk.PG

(Lin et al., 2015)

Enhancer of

Statement

Reference

Dhc64C^6-6 is an enhancer of eye phenotype of Egfr^E1

(Iyadurai et al., 2008)

Dhc64C^6-6/Dhc64C^6-8 is an enhancer of embryonic/first instar larval cuticle phenotype of baz⁴

(Harris and Peifer, 2005)

Suppressor of

Statement

Reference

Dhc64C^6-6/Dhc64C[+] is a suppressor of oocyte nucleus phenotype of Lis-1^E415

(Swan et al., 1999)

Dhc64C^6-6/Dhc64C[+] is a suppressor of oocyte phenotype of Lis-1^E415

(Swan et al., 1999)

Dhc64C^6-6/Dhc64C[+] is a suppressor of ommatidium phenotype of DCTN1-p150^Gl-1

(McGrail et al., 1995)

Dhc64C^6-6/Dhc64C[+] is a suppressor of eye phenotype of DCTN1-p150^Gl-1

(McGrail et al., 1995)

Additional Comments

Genetic Interactions

Statement

Reference

Dendrite pruning defects in Dhc64C^6-6/Df(3L)BSC436 pupae (16h after puparium formation) are not suppressed by spn-F^{8D.Scer\UAS.T:Avic\GFP-EGFP} expression driven by Scer\GAL4^ppk.PG.

(Lin et al., 2015)

The baz⁴/+ phenotype is enhanced in homozygous, heterozygous or trans-heterozygous Dhc64C^6-6 or Dhc64C^6-8 mutants, resulting in larger cuticle holes and an overall loss of cuticle.

(Harris and Peifer, 2005)

Dhc64C^6-6 is a strong dominant suppressor of the Lis-1^E415 homozygous phenotype, resulting in fertility, proper oocyte nucleus positioning and near normal oocyte growth.

(Swan et al., 1999)

Dhc64C^6-6/Df(3L)Gl2 flies have wild-type eyes. The rough eye phenotype of Gl¹/+ flies is dominantly suppressed by Dhc64C^6-6; ommatidia are shaped normally except in the most posterior region of the eye.

(McGrail et al., 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

Dhc64C^6-8

(Robinson et al., 1999)

Fails to complement

Dhc64C¹⁰²

(McGrail et al., 1995)

Rescued by

Dhc64C^6-6/Dhc64C^6-8 is rescued by Dhc64C^+tDN17

(Robinson et al., 1999)

Dhc64C^6-6/Df(3L)10^H is rescued by Dhc64C^+tDN17

(Robinson et al., 1999)

Comments

Dhc64C^6-6 and Dhc64C^6-8 complement each other for the zygotic requirement of Dhc64C function but result in maternal effect lethality.

(Robinson et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

32015

mwh¹ Dhc64C^6-6 hry¹ st¹ p^p ss¹ e^s/TM6B, Tb¹

Notes on Origin

Discoverer

Comments

Complements Dhc64C^8-1. Intragenic complementation relationships involve Dhc64C^5-6, Dhc64C^6-6, Dhc64C^6-8, Dhc64C^6-10, Dhc64C^6-12 and Dhc64C^8-1.

(Gepner et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

Dhc6-6

(Boylan et al., 2008, Mische et al., 2007)

Dhc64C6-6

(Mische et al., 2007)

Dhc64C^6-6

(Zhang et al., 2009, Cox and Spradling, 2006)

Dhc64c^6-6

(Palacios and St. Johnston, 2002)

Dhc6^6-6

(Iyadurai et al., 2008)

Dhc^6-6

(Lin et al., 2015, Parassol et al., 2013, Lerit and Gavis, 2011, Hamilton et al., 2009, Zimyanin et al., 2008, Abdu et al., 2006, Papoulas et al., 2005, Pare and Suter, 2000, Swan et al., 1999)

dhc64C^6-6

(Harris and Peifer, 2005)

dhc^6-6

(Clark et al., 2007, Macdougall et al., 2003, Vaccari and Ephrussi, 2002)

l(3)64Ca^6-6

Name Synonyms

Secondary FlyBase IDs

References (24)

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