FlyBase Allele Report: Dmel\Dhc64C[6-12]

General Information

Symbol

Dmel\Dhc64C^6-12

Species

D. melanogaster

Name

FlyBase ID

FBal0048194

Feature type

allele

Associated gene

Dmel\Dhc64C

Associated Insertion(s)

Carried in Construct

Also Known As

dhc^6-12, Dhc6-12

Key Links

Allele class

loss of function allele

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Abdu et al., 2006)

loss of function allele

(Gepner et al., 1996)

Mutagen

ethyl methanesulfonate

(Gepner et al., 1996)

Progenitor genotype

Cytology

Description

Genomic Southern blotting revealed no polymorphism between the mutant and wild type chromosome.

(Gepner et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile (with Dhc64C^6-6)

(Cox and Spradling, 2006, McGrail et al., 1995)

lethal | recessive

(McGrail and Hays, 1997, Gepner et al., 1996)

viable (with Dhc64C^6-6)

(Cox and Spradling, 2006)

Phenotype Manifest In

cyst cell of ovary

(Snapp et al., 2004)

egg

(McGrail and Hays, 1997)

egg chamber

(Snapp et al., 2004)

fusome

(McGrail and Hays, 1997)

germline cyst | germline clone (with Dhc64C^6-6)

(Cox and Spradling, 2006)

mitochondrial cloud | germline clone (with Dhc64C^6-6)

(Cox and Spradling, 2006)

oocyte

(McGrail and Hays, 1997)

oocyte (with Dhc64C^6-6)

(Palacios and St. Johnston, 2002, Pare and Suter, 2000)

ovary (with Dhc64C^6-6)

(Pare and Suter, 2000)

spindle

(McGrail and Hays, 1997)

Detailed Description

Statement

Reference

Cytoplasmic streaming occurs normally in the oocytes of Dhc64C^6-6/Dhc64C^6-12 mutants.

(Mische et al., 2007)

Dhc64C^6-6/Dhc64C^6-12 mutant clonal prefollicular cysts show premature mitochondrial accumulation at the middle of the fusome compared to wild-type cysts. The Balbiani body is reduced in the oocytes that develop from these clones.

(Cox and Spradling, 2006)

In the cysts of Dhc64C^6-12/Dhc64C^3-2 females, there are some fusome structures but these are fragmented and do not extend into all cells of the cyst. Additionally, the number of cyst divisions are reduced, resulting in egg chambers that contain fewer than 16 cells. The mitotic synchrony of these divisions is contained.

(Snapp et al., 2004)

Ooplasmic streaming still occurs in stage 9 Dhc64C^6-6/Dhc64C^6-12 oocytes but it is significantly slower than normal.

(Palacios and St. Johnston, 2002)

Ovaries from Dhc64C^6-6/Dhc64C^6-12 females show a range of phenotypes, including the failure of the oocyte to differentiate.

(Pare and Suter, 2000)

Dhc64C^3-2/Dhc64C^6-12 transheterozygous ovaries do not produce mature eggs; the egg chambers do not contain a developing oocyte and each cell develops with a polyploid nucleus like a nurse cell. Fusomes are present in the germ line cysts but they appear less branched than wild type. Normal fusome morphology is rescued with Dhc64C^+tDN17. The mitotic spindles appear randomly oriented within the cyst and frequently fail to become attached to one branch of the fusome. Dhc64C^6-6/Dhc64C^6-12 transheterozygous ovaries produce mature eggs, however, the eggs are fragile and show variable defects in size, shape and number and orientation of the chorionic appendages.

(McGrail and Hays, 1997)

Lethality acts during larval, pupal or pharate adult phase. Low levels (3-12%) of embryonic lethality are observed.

(Gepner et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Dhc64C^6-12 is a non-enhancer of phenotype of Lis-1^E415

(Swan et al., 1999)

NOT Suppressor of

Statement

Reference

Dhc64C^6-12 is a non-suppressor of phenotype of Lis-1^E415

(Swan et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Complements Dhc64C^8-1. Intragenic complementation relationships involve Dhc64C^5-6, Dhc64C^6-6, Dhc64C^6-8, Dhc64C^6-10, Dhc64C^6-12 and Dhc64C^8-1.

(Gepner et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Dhc6-12

(Boylan et al., 2008, Mische et al., 2007)

Dhc64C6-12

(Mische et al., 2007)

Dhc64C^6-12

(Abdu et al., 2006, Cox and Spradling, 2006)

Dhc64c^6-12

(Palacios and St. Johnston, 2002)

Dhc^6-12

(Parassol et al., 2013, Lerit and Gavis, 2011, Hamilton et al., 2009, Zimyanin et al., 2008, Weil et al., 2006, Pare and Suter, 2000, Swan et al., 1999)

dhc^6-12

(Jaramillo et al., 2008, Clark et al., 2007, Macdougall et al., 2003, Vaccari and Ephrussi, 2002)

l(3)64Ca^6-12

Name Synonyms

Secondary FlyBase IDs

References (20)

Report Sections

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