FlyBase Allele Report: Dmel\Dhc64C[6-8]

General Information

Symbol

Dmel\Dhc64C^6-8

Species

D. melanogaster

Name

FlyBase ID

FBal0048197

Feature type

allele

Associated gene

Dmel\Dhc64C

Associated Insertion(s)

Carried in Construct

Also Known As

dhc^6-8

Key Links

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Gepner et al., 1996)

Mutagen

ethyl methanesulfonate

(Gepner et al., 1996)

Progenitor genotype

Cytology

Description

Genomic Southern blotting revealed no polymorphism between the mutant and wild type chromosome.

(Gepner et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Gepner et al., 1996)

lethal - all die during embryonic stage | maternal effect | recessive

(Robinson et al., 1999)

some die during embryonic stage | recessive | maternal effect | recessive

(Robinson et al., 1999)

viable (with Dhc64C¹⁰²)

(McGrail et al., 1995)

Phenotype Manifest In

Detailed Description

Statement

Reference

Dhc64C^6-6/Dhc64C^6-8 trans-heterozygous mutants are adult viable.

Dhc64C^6-6/Dhc64C^6-8 mutants (m/z mutants) produce large sheets of embryonic cuticle, indicative of relatively normal epithelial polarity.

(Harris and Peifer, 2005)

Embryos derived from Dhc64C^6-6/Dhc64C^6-8 females that develop to nuclear cycle 14 fail to undergo normal furrow formation that is seen between adjacent nuclei in wild-type embryos at this stage. These embryos also accumulate fewer Golgi bodies in the apical cytoplasm compared to wild-type embryos at this stage, despite the presence of intact microtubules.

(Papoulas et al., 2005)

Dhc64C^6-6/Dhc64C^6-8 adults are recovered in equal proportion to nonmutant siblings, indicating that this heteroallelic combination is not less viable than wild type. 94% of embryos derived from Dhc64C^6-6/Dhc64C^6-8 females mated to wild-type males fail to survive beyond the end of embryogenesis. Most of these embryos fail to cellularise properly and cannot complete gastrulation. Approximately 5.7% of the remaining embryos survive through hatching, with 1.4% of these embryos failing to complete larval development. The approximately 3% of embryos that complete larval development survive to adulthood. Two predominant mitotic defects, free centrosomes and multipolar spindle arrays, are found in syncytial blastoderm embryos derived from Dhc64C^6-6/Dhc64C^6-8 females mated to wild-type males that are arrested early in embryogenesis. Free centrosomes are seen either singly or in numbers. The free centrosomes can arise during both early and late nuclear cycles by different pathways that are independent of cell cycle stage. First, centrosomes are seen departing the nuclear envelope during prophase. The detachment of centrosomes from bipolar and multipolar spindles is also seen. Spindles are seen lacking one or both centrosomes at their poles. The mean distance between centrosomes and the associated spindle poles is 1.8μm, significantly higher than wild type. Centrosome migration is abnormal in these embryos. Spindle configurations are often excessively curved and the normally uniform spacing between spindles within the syncytium is disrupted. Multipolar spindle arrays are seen, which most commonly arise from fusion of a number of neighbouring spindles. Spindle-associated or single free centrosomes are also capable of inducing ectopic spindle poles on adjacent mitotic arrays. Abnormal spindles in which an apparently normal half-spindle containing a single centrosome, spindle pole and chromatin is flanked by an abnormally blunt-ended pole lacking a detectable centrosome are also seen. The mitotic defects are detected during very early nuclear cycles. The syncytial nuclear divisions of embryos derived from Dhc64C^6-6/Dhc64C^6-8 females mated to wild-type males show poor synchrony.

(Robinson et al., 1999)

Lethality acts during larval, pupal or pharate adult phase. Low levels (3-12%) of embryonic lethality are observed.

(Gepner et al., 1996)

Dhc64C^6-8/Df(3L)Gl2 flies have wild-type eyes.

(McGrail et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Dhc64C^6-6/Dhc64C^6-8 is an enhancer of lethal | embryonic stage phenotype of baz⁴

(Harris and Peifer, 2005)

Dhc64C^6-6/Dhc64C^6-8 is an enhancer of abnormal cell polarity phenotype of baz⁴

(Harris and Peifer, 2005)

Dhc64C[+]/Dhc64C^6-8 is an enhancer of visible | dominant phenotype of DCTN1-p150^Gl-1

(McGrail et al., 1995)

Phenotype Manifest In

Enhancer of

Statement

Reference

Dhc64C^6-6/Dhc64C^6-8 is an enhancer of embryonic/first instar larval cuticle phenotype of baz⁴

(Harris and Peifer, 2005)

Dhc64C[+]/Dhc64C^6-8 is an enhancer of eye phenotype of DCTN1-p150^Gl-1

(McGrail et al., 1995)

Dhc64C[+]/Dhc64C^6-8 is an enhancer of ommatidium phenotype of DCTN1-p150^Gl-1

(McGrail et al., 1995)

Additional Comments

Genetic Interactions

Statement

Reference

The baz⁴/+ phenotype is enhanced in homozygous, heterozygous or trans-heterozygous Dhc64C^6-6 or Dhc64C^6-8 mutants, resulting in larger cuticle holes and an overall loss of cuticle.

(Harris and Peifer, 2005)

The rough eye phenotype of Gl¹/+ flies is dominantly enhanced by Dhc64C^6-8; the eyes are reduced in size and ommatidia are frequently fused.

(McGrail et al., 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

Dhc64C^6-6

(Robinson et al., 1999)

Rescued by

Dhc64C^6-6/Dhc64C^6-8 is rescued by Dhc64C^+tDN17

(Robinson et al., 1999)

Dhc64C^6-8 is rescued by Dhc64C^+tDN17

(McGrail et al., 1995)

Comments

Dhc64C^6-6 and Dhc64C^6-8 complement each other for the zygotic requirement of Dhc64C function but result in maternal effect lethality.

(Robinson et al., 1999)

The enhancement of the Gl¹ phenotype by Dhc64C^6-8 is reversed if the flies also carry a copy of Dhc64C^+tDN17.

(McGrail et al., 1995)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Complements Dhc64C^8-1. Intragenic complementation relationships involve Dhc64C^5-6, Dhc64C^6-6, Dhc64C^6-8, Dhc64C^6-10, Dhc64C^6-12 and Dhc64C^8-1.

(Gepner et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Dhc64C^6-8

(Zhang et al., 2009)

Dhc^6-8

(Papoulas et al., 2005)

dhc64C^6-8

(Harris and Peifer, 2005)

dhc^6-8

(Macdougall et al., 2003)

l(3)64Ca^6-8

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

Open Close