FB2024_03 , released June 25, 2024
Allele: Dmel\msnDN.UAS
Open Close
General Information
Symbol
Dmel\msnDN.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0178813
Feature type
allele
Associated gene
Dmel\msn
Associated Insertion(s)
Carried in Construct
P{UAS-msn.DN}
Also Known As
UAS-DN-msn
Key Links
Transgenic product class
inframe_deletion
(Houalla et al., 2005)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Houalla et al., 2005)
Mutagen
in vitro construct
(Houalla et al., 2005)
Progenitor genotype
Carried in construct
P{UAS-msn.DN}
Cytology
Description

UASt sequences drive expression of msn in which the middle proline rich region (amino acids 330-768) has been deleted.

(Houalla et al., 2005)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
msn
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of msnDN.Scer\UAS under the control of Scer\GAL4twi.PG results in delayed or defective ventral furrow formation in a proportion of embryos.

      (Mathew et al., 2011)

      Expression of msnDN.Scer\UAS in the whole epidermis of embryos, driven by Scer\GAL4e22c, results in breaks in the localization of actin and myosin at the epithelial margin. These gaps correlate with reduced elongation and leading edge constriction of the affected cells. The same phenotype is seen when msnDN.Scer\UAS is expressed in the marginal cells of the epidermis using Scer\GAL4LE.

      Expression of msnDN.Scer\UAS in the amnioserosa cells of embryos, driven by Scer\GAL4c381, results in head problems in less than 10% of embryos, which vary from an anterior hole to dorsal-anterior holes.

      (Koppen et al., 2006)

      When msnDN.Scer\UAS is expressed in the developing eye of msn03349/+ animals a R-cell nuclear migration phenotype is seen in 47% of mutant eye discs. Only 5% of msn03349/+ eye discs show this phenotype on its own.

      (Houalla et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Scer\GAL4unspecified, msnDN.UAS has photoreceptor cell | precursor & nucleus phenotype, enhanceable by Df(2L)TW119

      (Houalla et al., 2005)

      Scer\GAL4unspecified, msnDN.UAS has photoreceptor cell | precursor & nucleus phenotype, enhanceable by Lis-1k13209

      (Houalla et al., 2005)

      Scer\GAL4unspecified, msnDN.UAS has photoreceptor cell | precursor & nucleus phenotype, enhanceable by BicDr5

      (Houalla et al., 2005)
      NOT Enhanced by
      Statement
      Reference

      Scer\GAL4unspecified, msnDN.UAS has photoreceptor cell | precursor & nucleus phenotype, non-enhanceable by Cdc423

      (Houalla et al., 2005)

      Scer\GAL4unspecified, msnDN.UAS has photoreceptor cell | precursor & nucleus phenotype, non-enhanceable by Pak6

      (Houalla et al., 2005)

      Scer\GAL4unspecified, msnDN.UAS has photoreceptor cell | precursor & nucleus phenotype, non-enhanceable by bsk2

      (Houalla et al., 2005)

      Scer\GAL4unspecified, msnDN.UAS has photoreceptor cell | precursor & nucleus phenotype, non-enhanceable by capuM732

      (Houalla et al., 2005)

      Scer\GAL4unspecified, msnDN.UAS has photoreceptor cell | precursor & nucleus phenotype, non-enhanceable by klarmarb-BX5

      (Houalla et al., 2005)
      NOT suppressed by
      Statement
      Reference

      Scer\GAL4twi.PG, msnDN.UAS has ventral furrow phenotype, non-suppressible | germline clone by bskunspecified

      (Mathew et al., 2011)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The defects in ventral furrow formation seen in embryos expressing msnDN.Scer\UAS under the control of Scer\GAL4twi.PG are not suppressed in embryos derived from bskunspecified female germline clones.

      (Mathew et al., 2011)

      42% of animals expressing msnDN.Scer\UAS in the developing eye, combined with BicDr5/+ exhibit a R-cell nuclear migration phenotype. 37% of animals expressing msnDN.Scer\UAS in the developing eye, combined with Df(2L)TW119/+ exhibit a R-cell nuclear migration phenotype. 7% of animals expressing msnDN.Scer\UAS in the developing eye, combined with bsk2/+ exhibit a R-cell nuclear migration phenotype. 0% of animals expressing msnDN.Scer\UAS in the developing eye, combined with capuM732/+ exhibit a R-cell nuclear migration phenotype. 0% of animals expressing msnDN.Scer\UAS in the developing eye, combined with Cdc423/+ exhibit a R-cell nuclear migration phenotype. 21% of animals expressing msnDN.Scer\UAS in the developing eye, combined with Lis-1k13209/+ exhibit a R-cell nuclear migration phenotype. 0% of animals expressing msnDN.Scer\UAS in the developing eye, combined with klarmarb-BX5/+ exhibit a R-cell nuclear migration phenotype. 0% of animals expressing msnDN.Scer\UAS in the developing eye, combined with Pak6/+ exhibit a R-cell nuclear migration phenotype.

      (Houalla et al., 2005)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      Scer\GAL4unspecified/msnDN.UAS fails to rescue msn03349/msn[+]

      (Houalla et al., 2005)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      msnDN.Scer\UAS
      msnDN.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (3)