FlyBase Allele Report: Dmel\msn[03349]

General Information

Symbol

Dmel\msn⁰³³⁴⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0009465

Feature type

allele

Associated gene

Dmel\msn

Associated Insertion(s)

P{PZ}msn⁰³³⁴⁹

Carried in Construct

Also Known As

msn^l(3)03349, msn-lacZ

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Kadrmas et al., 2004, Ruan et al., 2002, Ruan et al., 1999)

Mutagen

P-element activity

(Treisman and Rubin, 1996)

Progenitor genotype

Associated Insertion(s)

P{PZ}msn⁰³³⁴⁹

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:2,586,514..2,586,514 [+]

(Meister, 1998.2.10, Braun et al., 1997, Treisman and Rubin, 1996, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates multiple endocrine neoplasia type 2B

modeled by Ret^MEN2B.GMR

(Read et al., 2005)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult thorax

(Kadrmas et al., 2004)

embryonic/first instar larval cuticle | maternal effect

(Perrimon et al., 1996)

eye photoreceptor cell & axon

(Ruan et al., 1999)

eye photoreceptor cell & growth cone

(Ruan et al., 1999)

lamina

(Ruan et al., 1999)

lamina plexus

(Ruan et al., 2002)

medulla

(Ruan et al., 1999)

photoreceptor cell & axon

(Ruan et al., 2002)

photoreceptor cell | precursor & nucleus, with Scer\GAL4^unspecified, msn^DN.UAS

(Houalla et al., 2005)

photoreceptor cell R1 & axon (with msn¹⁰²)

(Ruan et al., 2002)

photoreceptor cell R2 & axon (with msn¹⁰²)

(Ruan et al., 2002)

photoreceptor cell R3 & axon (with msn¹⁰²)

(Ruan et al., 2002)

photoreceptor cell R4 & axon (with msn¹⁰²)

(Ruan et al., 2002)

photoreceptor cell R5 & axon (with msn¹⁰²)

(Ruan et al., 2002)

photoreceptor cell R6 & axon (with msn¹⁰²)

(Ruan et al., 2002)

Detailed Description

Statement

Reference

When msn^DN.Scer\UAS is expressed in the developing eye of wild-type animals R-cell precursors migrate as normal. When expressed in msn⁰³³⁴⁹/+ animals a highly penetrant nuclear migration phenotype is seen.

(Houalla et al., 2005)

Almost no msn⁰³³⁴⁹ homozygous flies eclose and a large proportion of those that do have thorax closure defects.

(Kadrmas et al., 2004)

Many R1-R6 growth cones fail to stop at the lamina layer in msn⁰³³⁴⁹/msn¹⁰² larvae. Most msn⁰³³⁴⁹ animals die at the late pupal stage. msn⁰³³⁴⁹ third instar larvae have a mild defect in photoreceptor cell axonal projections. The lamina plexus is uneven. Only 5% of msn⁰³³⁴⁹ animals expressing msn^{D160N.Scer\UAS} under the control of Scer\GAL4^elav.PLu survive to the pupal stage.

(Ruan et al., 2002)

Lethality acts during the larval and pupal stages.

(Sorrentino et al., 2002)

The photoreceptor cell growth cones are able to extend into the developing optic lobe in homozygous third instar larvae, but their innervation patterns within the lamina and medulla are altered compared to wild type. Gaps are seen in the lamina R1-R6 termination site and abnormal, large bundles of axons are seen in the medulla. Growth cone morphology is altered, although the photoreceptor cell growth cones are able to expand upon reaching the target. The severity of the phenotype is variable.

(Ruan et al., 1999)

Germline clones produce eggs with patterning defects: embryos are twisted with variable cuticle differentiation.

(Perrimon et al., 1996)

Homozygotes late to eclose.

(Berkeley Drosophila Genome Project, 1993.6.1)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

msn⁰³³⁴⁹ has partially lethal - majority die | recessive phenotype, suppressible by stck[+]/stck¹⁸

(Kadrmas et al., 2004)

msn⁰³³⁴⁹ has partially lethal - majority die | recessive phenotype, suppressible by ics^BG02577/ics^BG02577

(Kadrmas et al., 2004)

Enhancer of

Statement

Reference

msn⁰³³⁴⁹ is an enhancer of visible phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

Other

Statement

Reference

BicD^r5, msn⁰³³⁴⁹ has lethal | larval stage phenotype

(Houalla et al., 2005)

BicD[+]/BicD^r5, msn⁰³³⁴⁹ has lethal | larval stage phenotype

(Houalla et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

msn⁰³³⁴⁹ has eye photoreceptor cell & growth cone phenotype, enhanceable by dock¹³⁴²¹

(Ruan et al., 1999)

msn⁰³³⁴⁹ has lamina phenotype, enhanceable by dock¹³⁴²¹

(Ruan et al., 1999)

Suppressed by

Statement

Reference

msn⁰³³⁴⁹ has adult thorax phenotype, suppressible by stck[+]/stck¹⁸

(Kadrmas et al., 2004)

msn⁰³³⁴⁹ has adult thorax phenotype, suppressible by ics^BG02577/ics^BG02577

(Kadrmas et al., 2004)

Enhancer of

Statement

Reference

msn⁰³³⁴⁹ is an enhancer of eye phenotype of Ret^MEN2B.GMR

(Read et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Introduction of a single copy of stck¹⁸ into the homozygous msn⁰³³⁴⁹ homozygous background increases the incidence of eclosion from approximately 0% to approximately 15% and also suppresses the msn⁰³³⁴⁹ thorax closure defect.

Homozygosity for ics^BG02577 increases the incidence of eclosion seen in msn⁰³³⁴⁹ homozygotes from approximately 0.5% to approximately 3%, and completely suppresses the msn⁰³³⁴⁹ thorax closure defects.

(Kadrmas et al., 2004)

The phenotype is dominantly enhanced by dock¹³⁴²¹; the R1-R6 termination site at the lamina becomes more disorganised and photoreceptor cell growth cones are much less expanded.

(Ruan et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

msn⁰⁶⁹⁴⁶

(Meister, 1998.2.10)

Rescued by

msn⁰³³⁴⁹ is rescued by msn^UAS.cSa/Scer\GAL4^elav.PLu

(Ruan et al., 2002)

Not rescued by

msn⁰³³⁴⁹/msn[+] is not rescued by Scer\GAL4^unspecified/msn^DN.UAS

(Houalla et al., 2005)

msn⁰³³⁴⁹ is not rescued by msn^D160N.UAS/Scer\GAL4^elav.PLu

(Ruan et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: l(3)01629⁰¹⁶²⁹. Complements: l(3)06264⁰⁶²⁶⁴. Complements: mRpL12¹⁰⁵³⁴. Complements: Prm¹⁰⁶³¹. Complements: mRpL12^j4B2. Complements: l(3)neo14¹. Complements: mRpL12^rL424. Complements: l(3)s2383^s2383.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

l(3)03349⁰³³⁴⁹

msn⁰³³⁴⁹

msn³³⁴⁹

(Kadrmas et al., 2004)

msn^l(3)03349

(Houalla et al., 2005, Ruan et al., 2002, Ruan et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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