FB2024_04 , released June 25, 2024
Recombinant Construct: P{PZ}
Open Close
General Information
Symbol
P{PZ}
FlyBase ID
FBtp0000210
Feature type
transgenic_transposable_element
Component Allele(s)
l(3)87Df7.2
Size
14.545
Expression Data
Associated insertion(s)
1754 available
Molecular map
Description and Uses
Description

P{PZ} is an enhancer trap construct that encodes a lacZ reporter. The enhancer trap sequence consists of the weak P\T promoter and coding sequence for the first 125 P\T amino acids fused upstream of the lacZ coding sequence.

(Mlodzik and Hiromi, 1992)

The lacZ reporter encoded by the P{PZ} construct is expected to be localized to the nucleus, because it is fused to the N-terminal 125 amino acid residues of P\T (which have been shown to be sufficient for nuclear localization).

(FlyBase, 1992-)

A diagram of the P{PZ} element is available at the [GDP Transposons page](http://flypush.imgen.bcm.tmc.edu/pscreen/transposons.html)

(FlyBase, 1992-)
Construct components
Component
Use(s)
Element type
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Marker(s)
ry+t7.2
Component allele l(3)87Df7.2
Transgenic product class
Component
Use(s)
Encoded product / tool
l(3)87Df
Description

Construct: Portion of l(3)87Df containing an intron of 70 nucleotides, the splice acceptor site and the remaining coding sequence and transcription termination sequences.

(Horowitz and Berg, 1995)
Sequence Data
Sequence (FB)
FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size

14.545

Left end

P5\'

(Mlodzik and Hiromi, 1992)
Right end

P3\'

(Mlodzik and Hiromi, 1992)
 
Segments
Num
Orientation
Symbol
1
+
P\T::lacZ(P5',u)
2
+
001115a
3
+
001115b
4
-
neor1.3(H3,Sm)
5
+
001114
6
+
PSJ1_191
7
+
pHSS6_ori
8
+
001116
9
+
Car20_P3'(H3,P3')
Reference
(Mlodzik and Hiromi, 1992)
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

sequence comment:

(Mlodzik and Hiromi, 1992)

report comment: Thanks to Brian Calvi and Vladimir Benes for assistance in assembling compiled sequence. Included as non-attributed correction.

(Benes, 1997.7.25)

sequence comment: Included as non-attributed correction.

(Benes, 1997.7.25)

mapping_comment:

(Mlodzik and Hiromi, 1992)

report comment:

(Mlodzik and Hiromi, 1992)

mapping_comment: Included as non-attributed correction.

(Benes, 1997.7.25)

construct_comment: Restriction site useful for plasmid rescue: XbaI.

(Mlodzik and Hiromi, 1992)
Stocks (534)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (54)