FlyBase Allele Report: Dmel\dock[13421]

General Information

Symbol

Dmel\dock¹³⁴²¹

Species

D. melanogaster

Name

FlyBase ID

FBal0049421

Feature type

allele

Associated gene

Dmel\dock

Associated Insertion(s)

P{PZ}dock¹³⁴²¹

Carried in Construct

Also Known As

dock^P2

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Garrity et al., 1996)

Mutagen

P-element activity

(Garrity et al., 1996)

Progenitor genotype

Associated Insertion(s)

P{PZ}dock¹³⁴²¹

Cytology

Description

P{PZ} insertion 37bp downstream of the first exon-intron boundary.

(Garrity et al., 1996)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:826,544..826,544 [+]

(Garrity et al., 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Bolwig nerve (with dock⁰⁴⁷²³)

(Schmucker et al., 2000)

embryonic/larval optic lobe

(Song et al., 2003)

embryonic/larval somatic muscle cell

(Desai et al., 1999)

lamina anlage

(Song et al., 2003, Hummel et al., 2000)

larval longitudinal connective

(Hummel et al., 2000, Desai et al., 1999)

larval longitudinal connective | maternal effect

(Desai et al., 1999)

larval ventral nerve cord commissure

(Hummel et al., 2000)

larval VUM neuron

(Hummel et al., 2000)

medulla anlage

(Song et al., 2003)

photoreceptor cell & axon

(Hummel et al., 2000, Garrity et al., 1996)

RP3 neuron & synapse

(Desai et al., 1999)

Detailed Description

Statement

Reference

dock¹³⁴²¹/+ mutant third instar larval eye discs do not show disrupted axon targeting from photoreceptor neurons to the brain optic lobes.

(Shcherbata et al., 2007)

When mutant clones are made in the developing eye, no R-cell precursor nuclear migration phenotype was seen.

(Houalla et al., 2005)

Photoreceptor cell axons fail to target the optic lobe properly in mutant larvae; crossing of axons, gaps in the lamina and blunt ended termination point in the medulla are seen.

(Song et al., 2003)

The growth of retinula axons into the brain is disrupted in homozygous larvae, leading to a disruption of the lamina neuropile organisation. Homozygous embryos show a reduction in the longitudinal connectives and a slight fusion of the segmental commissures. The VUM neurons do not project normally.

(Hummel et al., 2000)

In dock⁰⁴⁷²³/dock¹³⁴²¹ embryos, either the entire Bolwig's Nerve, or a subset of its axons project to ectopic positions. These defects have over a 90% penetrance.

(Schmucker et al., 2000)

In dock¹³⁴²¹/dock³ mutant embryos longitudinal connectives are more wavy and more varied in thickness than wild type and the outermost one is occasionally interrupted. There are low penetrance defects in muscle organization. The muscle phenotype is not affected further in embryos lacking both maternal and zygotic dock activity. dock¹³⁴²¹/dock³ mutant embryos (and homozygotes of both alleles) show a variable absence of the synapse between RP3 and muscles 7 and 6. Outgrowth of RP3 from the CNS is normal. Synapse formation eventually occurs, but is delayed. No ectopic synapses occur. This phenotype is identical to that of lbm^Y13. Maternal loss of dock expression enhances the CNS longitudinal axon defects, but not the RP3 synapse formation delay.

(Desai et al., 1999)

Some homozygotes survive to adulthood. These flies are sluggish and uncoordinated, dying within a few days after eclosion. Homozygous third instar larvae exhibit defects in receptor cell fasciculation, targeting and retinotopy. Receptor cell axons terminate at different depths in the developing lamina and form clumps of terminals instead of an even array. Bundles project from these regions into the medulla. Some bundles project along abnormal paths.

(Garrity et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

dock¹³⁴²¹ is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

NOT Suppressor of

Statement

Reference

dock¹³⁴²¹ is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

Other

Statement

Reference

Dg³²³, dock[+]/dock¹³⁴²¹ has abnormal neuroanatomy | third instar larval stage phenotype

(Shcherbata et al., 2007)

InR^ex15, dock¹³⁴²¹ has abnormal neuroanatomy phenotype

(Song et al., 2003)

InR[+]/InR^ex15, dock¹³⁴²¹ has abnormal neuroanatomy phenotype

(Song et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

dock¹³⁴²¹ has photoreceptor cell & axon phenotype, enhanceable by Hem[+]/Hem^Δ2-6

(Hummel et al., 2000)

NOT Enhanced by

Statement

Reference

dock¹³⁴²¹ has phenotype, non-enhanceable by lbm^Y13

(Desai et al., 1999)

NOT suppressed by

Statement

Reference

dock¹³⁴²¹ has phenotype, non-suppressible by lbm^Y13

(Desai et al., 1999)

Enhancer of

Statement

Reference

dock[+]/dock¹³⁴²¹ is an enhancer of photoreceptor cell & axon phenotype of Hem^Δ2-6

(Hummel et al., 2000)

dock¹³⁴²¹ is an enhancer of eye photoreceptor cell & growth cone phenotype of msn⁰³³⁴⁹

(Ruan et al., 1999)

dock¹³⁴²¹ is an enhancer of lamina phenotype of msn⁰³³⁴⁹

(Ruan et al., 1999)

NOT Enhancer of

Statement

Reference

dock¹³⁴²¹ is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

NOT Suppressor of

Statement

Reference

dock¹³⁴²¹ is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

Other

Statement

Reference

Dg³²³, dock[+]/dock¹³⁴²¹ has eye disc | third instar larval stage phenotype

(Shcherbata et al., 2007)

Dg³²³, dock[+]/dock¹³⁴²¹ has eye photoreceptor cell | third instar larval stage phenotype

(Shcherbata et al., 2007)

Dg³²³, dock[+]/dock¹³⁴²¹ has lamina plexus | third instar larval stage phenotype

(Shcherbata et al., 2007)

InR^ex15, dock¹³⁴²¹ has medulla anlage phenotype

(Song et al., 2003)

InR^ex15, dock¹³⁴²¹ has medulla phenotype

(Song et al., 2003)

InR[+]/InR^ex15, dock¹³⁴²¹ has medulla anlage phenotype

(Song et al., 2003)

InR[+]/InR^ex15, dock¹³⁴²¹ has medulla phenotype

(Song et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

dock¹³⁴²¹/+; Dys^8-2/+ double heterozygous mutant third instar larval eye discs do not show disrupted axon targeting from photoreceptor neurons to the brain optic lobes.

dock¹³⁴²¹/+; Df(3R)Dl-X43/+ double heterozygous mutant third instar larval eye discs do not show disrupted axon targeting from photoreceptor neurons to the brain optic lobes.

Dg³²³/dock¹³⁴²¹ double heterozygous mutant third instar larval eye discs show disrupted axon targeting from photoreceptor neurons to the brain optic lobes.

(Shcherbata et al., 2007)

dock¹³⁴²¹/+ ; InR^ex15/+ double heterozygotes show defects in photoreceptor axon projections in the medulla; gaps in the R7 terminal array and uninnervated areas are seen. These defects are seen in larvae and adults.

(Song et al., 2003)

One copy of dock¹³⁴²¹ enhances the axonal projection phenotype of Hem^Δ2-6 homozygous larvae. One copy of Hem^Δ2-6 enhances the axonal projection phenotype of dock¹³⁴²¹ homozygous larvae.

(Hummel et al., 2000)

The msn⁰³³⁴⁹ R1-R6 photoreceptor cell phenotype is dominantly enhanced by dock¹³⁴²¹; the R1-R6 termination site at the lamina becomes more disorganised and photoreceptor cell growth cones are much less expanded.

(Ruan et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

dock¹³⁴²¹/dock⁰⁴⁷²³ is rescued by dock^GMR.PG

(Schmucker et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dock¹³⁴²¹

dock^P13421

(Zhu et al., 2005)

dock^P2

(Shcherbata et al., 2007, Houalla et al., 2005, Song et al., 2003, Hummel et al., 2000, Schmucker et al., 2000, Desai et al., 1999, Ruan et al., 1999, Garrity et al., 1996)

Name Synonyms

Secondary FlyBase IDs

References (9)

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