FlyBase Allele Report: Dmel\msl-2[Hsp83.PK]

General Information

Symbol

Dmel\msl-2^Hsp83.PK

Species

D. melanogaster

Name

Heat shock protein 83 promoter construct of Kelley

FlyBase ID

FBal0044201

Feature type

allele

Associated gene

Dmel\msl-2

Associated Insertion(s)

Carried in Construct

P{H83M2}

Also Known As

H83M2, hsp83-msl2, [w⁺H83M2], H83MSL2

Key Links

Transgenic product class

wild_type

(Kelley et al., 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Kelley et al., 1995)

Mutagen

in vitro construct

(Chang and Kuroda, 1998, Amrein and Axel, 1997, Meller et al., 1997, Kelley et al., 1995)

Progenitor genotype

Carried in construct

P{H83M2}

Cytology

Description

msl-2 open reading frame from nucleotides 255-2978, lacking the internal 52 nucleotide intron and the putative Sxl binding sites in the 5' and 3' UTR of the transcripts, is expressed under the control of the Hsp83 promoter.

(Kelley et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp83

Encoded product / tool

msl-2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Females expressing msl-2^Hsp83.PK show a developmental delay (the peak of eclosion is delayed).

(Menon and Meller, 2012)

Female escapers carrying msl-2^Hsp83.PK lay few eggs and are sterile.

(Prabhakaran and Kelley, 2012)

Females expressing msl-2^Hsp83.PK show developmental delay and decreased viability compared to controls.

(Furuhashi et al., 2006)

The X chromosomes of females carrying msl-2^Hsp83.PK have a very diffuse morphology and very strongly reduced frequencies of weak spots on polytene X chromosomes, but not on polytene autosomes.

(Alekseyenko et al., 2002)

msl-2^Hsp83.PK females have low viability and, sterility and delayed development.

(Meller and Rattner, 2002)

11% of females carrying msl-2^Hsp83.PK are viable. Females carrying msl-2^Hsp83.PK which survive to adulthood show a delay in eclosion.

(Chang and Kuroda, 1998)

Females carrying msl-2^Hsp83.PK have reduced viability and fertility, and their development is delayed.

(Amrein and Axel, 1997)

Females exhibit a very long developmental delay and a significant loss of viability. Normal development is restored by removing one functional copy of mof.

(Hilfiker et al., 1997)

Dominant female specific developmental delay causing reduced viability and fertility.

(Kelley et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

msl-1^Hsp83.PC, msl-2^Hsp83.PK has partially lethal - majority die | male phenotype, enhanceable by Df(1)52/roX1[+]/Polr2A^+tcos4Δ4.3/lncRNA:roX1^ex84A/+

(Deng et al., 2005)

msl-1^Hsp83.PC, msl-2^Hsp83.PK has partially lethal - majority die | male phenotype, enhanceable by Df(1)52/roX1[+]/lncRNA:roX1^ex6/Polr2A^+tcos4Δ4.3/+

(Deng et al., 2005)

msl-1^Hsp83.PC, msl-2^Hsp83.PK has partially lethal - majority die | male phenotype, enhanceable by Df(1)52/roX1[+]/lncRNA:roX1^SMC17A/Polr2A^+tcos4Δ4.3/+

(Deng et al., 2005)

Suppressed by

Statement

Reference

msl-2^Hsp83.PK has lethal | female | heat sensitive phenotype, suppressible by Df(1)52/lncRNA:roX1^ex6/P{w⁺4Δ4.3}

(Lim and Kelley, 2012)

msl-2^Hsp83.PK has lethal | female | heat sensitive phenotype, suppressible by Df(1)52/P{w⁺4Δ4.3}

(Lim and Kelley, 2012)

msl-2^Hsp83.PK has abnormal developmental rate | female phenotype, suppressible by AGO2⁴¹⁴/AGO2[+]

(Menon and Meller, 2012)

msl-2^Hsp83.PK has abnormal developmental rate | female phenotype, suppressible by Spt5[+]/Spt5^880C

(Prabhakaran and Kelley, 2012)

msl-2^Hsp83.PK has female sterile phenotype, suppressible by Spt5[+]/Spt5^880C

(Prabhakaran and Kelley, 2012)

msl-2^Hsp83.PK has lethal | female phenotype, suppressible by Df(3L)Rac1/+

(Furuhashi et al., 2006)

msl-1^Hsp83.PC, msl-2^Hsp83.PK has lethal | female limited phenotype, suppressible | partially by Df(1)52/roX1[+]/Polr2A^+tcos4Δ4.3/lncRNA:roX1^ex84A/+

(Deng et al., 2005)

msl-1^Hsp83.PC, msl-2^Hsp83.PK has lethal | female limited phenotype, suppressible | partially by Df(1)52/roX1[+]/lncRNA:roX1^ex6/+

(Deng et al., 2005)

Df(1)52, Polr2A^+tcos4Δ4.3, msl-2^Hsp83.PK has partially lethal phenotype, suppressible by lncRNA:roX1^ex6

(Meller and Rattner, 2002)

msl-2^Hsp83.PK has abnormal developmental rate | female phenotype, suppressible by lncRNA:roX1^ex6

(Meller and Rattner, 2002)

msl-2^Hsp83.PK has abnormal developmental rate | female phenotype, suppressible by Df(1)52/P{w⁺4Δ4.3}

(Meller and Rattner, 2002)

msl-2^Hsp83.PK has partially lethal phenotype, suppressible by lncRNA:roX1^ex6

(Meller and Rattner, 2002)

msl-2^Hsp83.PK has partially lethal phenotype, suppressible by Df(1)52/Polr2A^+tcos4Δ4.3

(Meller and Rattner, 2002)

Df(1)52, P{w⁺4Δ4.3}, msl-2^Hsp83.PK has abnormal developmental rate | female phenotype, suppressible by lncRNA:roX1^ex6

(Meller and Rattner, 2002)

NOT suppressed by

Statement

Reference

msl-2^Hsp83.PK has lethal | female | heat sensitive phenotype, non-suppressible by lncRNA:roX1^ex6

(Lim and Kelley, 2012)

Enhancer of

Statement

Reference

msl-2^Hsp83.PK/msl-1^Hsp83.PC is an enhancer of lethal phenotype of lncRNA:roX1^ex6

(Oh et al., 2003)

msl-2^Hsp83.PK is an enhancer of abnormal sex-determination phenotype of Sxl^Mf1

(Cline et al., 1999)

Suppressor of

Statement

Reference

msl-2^Hsp83.PK/msl-1^Hsp83.PC is a suppressor | partially of partially lethal - majority die | male phenotype of Df(1)52, Polr2A^+tcos4Δ4.3, lncRNA:roX1^ex6

(Deng et al., 2005)

msl-2^Hsp83.PK/msl-1^Hsp83.PC is a suppressor | partially of lethal | male phenotype of Df(1)52, Polr2A^+tcos4Δ4.3, lncRNA:roX1^ex84A

(Deng et al., 2005)

msl-2^Hsp83.PK/msl-1^Hsp83.PC is a suppressor | partially of lethal | male | pupal stage phenotype of Df(1)52, Polr2A^+tcos4Δ4.3, lncRNA:roX1^SMC17A

(Deng et al., 2005)

msl-2^Hsp83.PK is a suppressor | partially of lethal | male phenotype of Trl^2.3/Trl^13C

(Greenberg et al., 2004)

msl-2^Hsp83.PK is a suppressor | partially of lethal | male phenotype of Trl⁶²/Trl^13C

(Greenberg et al., 2004)

msl-2^Hsp83.PK/msl-1^Hsp83.PC is a suppressor | partially of lethal phenotype of Df(1)52, lncRNA:roX1^ex6

(Oh et al., 2003)

Other

Statement

Reference

msl-1^Hsp83.PC, msl-2^Hsp83.PK has partially lethal - majority die | male phenotype

(Deng et al., 2005)

msl-1^Hsp83.PC, msl-2^Hsp83.PK has lethal | female limited phenotype

(Deng et al., 2005)

Trl⁶²/Trl^13C, msl-2^Hsp83.PK has partially lethal phenotype

(Greenberg et al., 2004)

Trl^2.3/Trl^13C, msl-2^Hsp83.PK has partially lethal phenotype

(Greenberg et al., 2004)

Trl^13C, msl-2^Hsp83.PK has partially lethal phenotype

(Greenberg et al., 2004)

Sxl^M12, msl-2^Hsp83.PK has abnormal sex-determination phenotype

(Cline et al., 1999)

msl-1^γ269, msl-1^+tFL, msl-2^Hsp83.PK has visible | female phenotype

(Chang and Kuroda, 1998)

msl-1^γ269, msl-1^MPU, msl-2^Hsp83.PK has visible | female phenotype

(Chang and Kuroda, 1998)

msl-1^γ269, msl-1^+tT3UTR, msl-2^Hsp83.PK has visible | female phenotype

(Chang and Kuroda, 1998)

msl-1^MPU, msl-2^Hsp83.PK has visible | female phenotype

(Chang and Kuroda, 1998)

msl-1^+tT3UTR, msl-2^Hsp83.PK has visible | female phenotype

(Chang and Kuroda, 1998)

msl-1^+tFL, msl-2^Hsp83.PK has visible | female phenotype

(Chang and Kuroda, 1998)

msl-1^Hsp83.PC, msl-2^Hsp83.PK has lethal | female | larval stage phenotype

(Chang and Kuroda, 1998)

msl-1^γ269, msl-2^Hsp83.PK has viable | female phenotype

(Chang and Kuroda, 1998)

Phenotype Manifest In

Enhancer of

Statement

Reference

msl-2^Hsp83.PK is an enhancer of genitalia phenotype of Sxl^Mf1

(Cline et al., 1999)

msl-2^Hsp83.PK is an enhancer of prothoracic leg phenotype of Sxl^Mf1

(Cline et al., 1999)

NOT Enhancer of

Statement

Reference

msl-2^Hsp83.PK/msl-2[+] is a non-enhancer of eye phenotype of hid^GMR.cLa

(Laverty et al., 2011)

Suppressor of

Statement

Reference

msl-2^Hsp83.PK is a suppressor of phenotype of msl-1^{Δ84.Tag:FLAG}

(Scott et al., 2000)

msl-2^Hsp83.PK is a suppressor of phenotype of msl-1^Δ84C.hs

(Scott et al., 2000)

msl-2^Hsp83.PK is a suppressor of phenotype of msl-1^{FN.hs.Tag:FLAG}

(Scott et al., 2000)

msl-2^Hsp83.PK is a suppressor of abdomen phenotype of Sxl^M12

(Cline et al., 1999)

msl-2^Hsp83.PK is a suppressor of phenotype of Sxl^FL.Hsp83

(Yanowitz et al., 1999)

msl-2^Hsp83.PK is a suppressor of phenotype of Sxl^NΔ.Hsp83

(Yanowitz et al., 1999)

NOT Suppressor of

Statement

Reference

msl-2^Hsp83.PK/msl-2[+] is a non-suppressor of eye phenotype of hid^GMR.cLa

(Laverty et al., 2011)

msl-2^Hsp83.PK is a non-suppressor of phenotype of Sxl^FLΔ.Hsp83

(Yanowitz et al., 1999)

Other

Statement

Reference

msl-1^Hsp83.PC, msl-2^Hsp83.PK has nuclear chromosome phenotype

(Oh et al., 2003)

Iswi², msl-2^Hsp83.PK has X chromosome | female phenotype

(Corona et al., 2002)

Sxl^M12, msl-2^Hsp83.PK has abdominal tergite 5 phenotype

(Cline et al., 1999)

Sxl^M12, msl-2^Hsp83.PK has sex comb phenotype

(Cline et al., 1999)

Sxl^M12, msl-2^Hsp83.PK has prothoracic leg phenotype

(Cline et al., 1999)

Sxl^M12, msl-2^Hsp83.PK has abdominal tergite 6 phenotype

(Cline et al., 1999)

msl-1^γ269, msl-1^+tFL, msl-2^Hsp83.PK has wing phenotype

(Chang and Kuroda, 1998)

msl-1^γ269, msl-1^MPU, msl-2^Hsp83.PK has wing phenotype

(Chang and Kuroda, 1998)

msl-1^γ269, msl-1^+tT3UTR, msl-2^Hsp83.PK has wing phenotype

(Chang and Kuroda, 1998)

msl-1^MPU, msl-2^Hsp83.PK has wing phenotype

(Chang and Kuroda, 1998)

msl-1^+tT3UTR, msl-2^Hsp83.PK has wing phenotype

(Chang and Kuroda, 1998)

msl-1^+tFL, msl-2^Hsp83.PK has wing phenotype

(Chang and Kuroda, 1998)

Additional Comments

Genetic Interactions

Statement

Reference

AGO2⁴¹⁴/+ partially suppresses the delay in peak eclosion which is seen in females expressing msl-2^Hsp83.PK.

(Menon and Meller, 2012)

Spt5^880C/+ modestly rescues the developmental delay of females carrying msl-2^Hsp83.PK. The rescued females produce abundant eggs that successfully develop into larvae.

(Prabhakaran and Kelley, 2012)

W^GMR.cLa/+; msl-2^Hsp83.PK/+ flies are identical to W^GMR.cLa/+ flies.

(Laverty et al., 2011)

The expression of msl-2^Hsp83.PK in females overexpressing Su(var)3-7^{FL.hs.T:Ivir\HA1} causes the X chromosomes to condense as typically seen only in males overexpressing Su(var)3-7^{FL.hs.T:Ivir\HA1}.

A Su(var)3-7^R2a8 background kills males expressing msl-1^Hsp83.PC-msl-2^Hsp83.PK. Homozygous Su(var)3-7^R2a8 females crossed with transgenic msl-1^Hsp83.PC-msl-2^Hsp83.PK males produce only 6% of male adult progeny harbouring the transgenes compared to 48% if the mothers are heterozygous for Su(var)3-7^R2a8.

(Spierer et al., 2008)

When msl-1^Hsp83.PC and msl-2^Hsp83.PK are expressed in wild-type animals, short wide X chromosomes are seen. The addition of msl-1^Hsp83.PC and msl-2^Hsp83.PK to roX1^ex6 males reduces their viability. roX1^ex6, Df(1)52 males have a very low viability, only up to 6.6% of males survive. This loss of viability is partially rescued by adding msl-1^Hsp83.PC and msl-2^Hsp83.PK, with up to 45% of males surviving.

(Oh et al., 2003)

Expression of msl-2^Hsp83.PK in a Iswi² background (but not in a wild-type background) results in the X chromosome having a bloated morphology in salivary gland polytene chromosome preparations from female larvae. In approximately 30% of the salivary gland nuclei, the phenotype is so severe that the X chromosome is almost completely dispersed and detaches from the chromocenter.

(Corona et al., 2002)

The addition of msl-2^Hsp83.PK suppresses the male viability phenotype seen in heatshocked msl-1^{Δ84.T:Zzzz\FLAG}, msl-1^Δ84C.hs and msl-1^{FN.hs.T:Zzzz\FLAG} flies.

(Scott et al., 2000)

Addition of msl-2^Hsp83.PK leads to partial suppression of the abdominal etching phenotype seen in Sxl^M12/Y males. For Sxl^M12/Y males with msl-2^Hsp83.PK, 72% of their fifth and sixth hemitergites were completely feminized. In contrast none of their forelegs were fully female and 77% were entirely male. The addition of msl-2^Hsp83.PK to Sxl^Mf1/Y flies leads to males with completely female genitalia and forelegs, yet nearly normal abdomens.

(Cline et al., 1999)

Suppresses the Sxl^FL.Hsp83 male lethality in both Sxl⁺ and Sxl^fP7B0 backgrounds.

(Yanowitz et al., 1999)

Females carrying both msl-1^Hsp83.PC and msl-2^Hsp83.PK do not survive, dying during late larval development. Males carrying both msl-1^Hsp83.PC and msl-2^Hsp83.PK are fully viable. 72% of msl-1^γ269/+ females carrying msl-1^Hsp83.PC are viable. msl-1^γ269/+ females carrying both msl-1^Hsp83.PC and msl-2^Hsp83.PK survive to the third larval instar stage. The developmental delay of females carrying msl-2^Hsp83.PK is further increased if they are also carrying msl-1^MPU or msl-1^+tT3UTR, but is unaffected if they are also carrying msl-1^+tFL. msl-1^γ269/+ females carrying msl-2^Hsp83.PK and either msl-1^+tFL, msl-1^MPU or msl-1^+tT3UTR often have a scalloped wing phenotype.

(Chang and Kuroda, 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Basal level of expression is sufficient to rescue msl-2 mutant males. Female specific toxic effects of induced expression can be suppressed by removing functional msl-1, mle or msl-3.

(Kelley et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

msl-2^Hsp83.PK

(Dahlsveen et al., 2006)

msl-2^cnstv

(Cline et al., 1999)

Name Synonyms

Heat shock protein 83 promoter construct of Kelley

(Kelley et al., 1995)

Secondary FlyBase IDs

References (43)

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