FlyBase Allele Report: Dmel\msl-1[Hsp83.PC]

General Information

Symbol

Dmel\msl-1^Hsp83.PC

Species

D. melanogaster

Name

Heat shock protein 83 promoter construct of Chang

FlyBase ID

FBal0093826

Feature type

allele

Associated gene

Dmel\msl-1

Associated Insertion(s)

Carried in Construct

P{Hsp83-msl-1.C}

Key Links

Transgenic product class

wild_type

(Chang and Kuroda, 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Chang and Kuroda, 1998)

Mutagen

in vitro construct

(Chang and Kuroda, 1998)

Progenitor genotype

Carried in construct

P{Hsp83-msl-1.C}

Cytology

Description

The Hsp83 promoter drives expression of the msl-1 coding region (the 5' and 3' UTRs and the intron have been removed).

(Chang and Kuroda, 1998)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp83

Encoded product / tool

msl-1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable

(Spierer et al., 2008)

viable | female

(Chang and Kuroda, 1998)

wild-type

(Chang and Kuroda, 1998)

Phenotype Manifest In

Detailed Description

Statement

Reference

Females carrying msl-1^Hsp83.PC are viable.

(Chang and Kuroda, 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

msl-1^Hsp83.PC, msl-2^Hsp83.PK has partially lethal - majority die | male phenotype, enhanceable by Df(1)52/roX1[+]/Polr2A^+tcos4Δ4.3/lncRNA:roX1^ex84A/+

(Deng et al., 2005)

msl-1^Hsp83.PC, msl-2^Hsp83.PK has partially lethal - majority die | male phenotype, enhanceable by Df(1)52/roX1[+]/lncRNA:roX1^ex6/Polr2A^+tcos4Δ4.3/+

(Deng et al., 2005)

msl-1^Hsp83.PC, msl-2^Hsp83.PK has partially lethal - majority die | male phenotype, enhanceable by Df(1)52/roX1[+]/lncRNA:roX1^SMC17A/Polr2A^+tcos4Δ4.3/+

(Deng et al., 2005)

Suppressed by

Statement

Reference

msl-1^Hsp83.PC, msl-2^Hsp83.PK has lethal | female limited phenotype, suppressible | partially by Df(1)52/roX1[+]/Polr2A^+tcos4Δ4.3/lncRNA:roX1^ex84A/+

(Deng et al., 2005)

msl-1^Hsp83.PC, msl-2^Hsp83.PK has lethal | female limited phenotype, suppressible | partially by Df(1)52/roX1[+]/lncRNA:roX1^ex6/+

(Deng et al., 2005)

Enhancer of

Statement

Reference

msl-1^Hsp83.PC is an enhancer of visible | female limited phenotype of msl-2^ey.hs

(Kelley and Kuroda, 2003)

msl-2^Hsp83.PK/msl-1^Hsp83.PC is an enhancer of lethal phenotype of lncRNA:roX1^ex6

(Oh et al., 2003)

Suppressor of

Statement

Reference

msl-2^Hsp83.PK/msl-1^Hsp83.PC is a suppressor | partially of partially lethal - majority die | male phenotype of Df(1)52, Polr2A^+tcos4Δ4.3, lncRNA:roX1^ex6

(Deng et al., 2005)

msl-2^Hsp83.PK/msl-1^Hsp83.PC is a suppressor | partially of lethal | male phenotype of Df(1)52, Polr2A^+tcos4Δ4.3, lncRNA:roX1^ex84A

(Deng et al., 2005)

msl-2^Hsp83.PK/msl-1^Hsp83.PC is a suppressor | partially of lethal | male | pupal stage phenotype of Df(1)52, Polr2A^+tcos4Δ4.3, lncRNA:roX1^SMC17A

(Deng et al., 2005)

msl-2^Hsp83.PK/msl-1^Hsp83.PC is a suppressor | partially of lethal phenotype of Df(1)52, lncRNA:roX1^ex6

(Oh et al., 2003)

Other

Statement

Reference

msl-1^Hsp83.PC, msl-2^Hsp83.PK has partially lethal - majority die | male phenotype

(Deng et al., 2005)

msl-1^Hsp83.PC, msl-2^Hsp83.PK has lethal | female limited phenotype

(Deng et al., 2005)

msl-1^Hsp83.PC, msl-2^Hsp83.PK has lethal | female | larval stage phenotype

(Chang and Kuroda, 1998)

Phenotype Manifest In

Enhancer of

Statement

Reference

msl-1^Hsp83.PC is an enhancer of eye phenotype of msl-2^ey.hs

(Kelley and Kuroda, 2003)

Other

Statement

Reference

msl-1^Hsp83.PC, msl-2^Hsp83.PK has nuclear chromosome phenotype

(Oh et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

A Su(var)3-7^R2a8 background kills males expressing msl-1^Hsp83.PC-msl-2^Hsp83.PK. Homozygous Su(var)3-7^R2a8 females crossed with transgenic msl-1^Hsp83.PC-msl-2^Hsp83.PK males produce only 6% of male adult progeny harbouring the transgenes compared to 48% if the mothers are heterozygous for Su(var)3-7^R2a8.

(Spierer et al., 2008)

Only 0.5% of msl-1^Hsp83.PC; msl-2^Hsp83.PK females survive to adulthood, compared with 36% of males. Female lethality is partially suppressed if these females carry P{w⁺4Δ4.3} and are heterozygous for Df(1)52 with roX1^ex84A or roX1^ex6. Male semi-lethality, however, is enhanced if these males carry P{w⁺4Δ4.3} and are heterozygous for Df(1)52 with roX1^ex84A, roX1^ex6 or roX1^SMC17A.

(Deng et al., 2005)

The rough eye phenotype seen in females expressing msl-2^ey.hs is strongly enhanced by co-expression of msl-1^Hsp83.PC, but males expressing both msl-2^ey.hs and msl-1^Hsp83.PC have wild-type eyes.

(Kelley and Kuroda, 2003)

When msl-1^Hsp83.PC and msl-2^Hsp83.PK are expressed in wild-type animals, short wide X chromosomes are seen. The addition of msl-1^Hsp83.PC and msl-2^Hsp83.PK to roX1^ex6 males reduces their viability. roX1^ex6, Df(1)52 males have a very low viability, only up to 6.6% of males survive. This loss of viability is partially rescued by adding msl-1^Hsp83.PC and msl-2^Hsp83.PK, with up to 45% of males surviving.

(Oh et al., 2003)

Females carrying both msl-1^Hsp83.PC and msl-2^Hsp83.PK do not survive, dying during late larval development. Males carrying both msl-1^Hsp83.PC and msl-2^Hsp83.PK are fully viable. msl-1^γ269/+ females carrying both msl-1^Hsp83.PC and msl-2^Hsp83.PK survive to the third larval instar stage.

(Chang and Kuroda, 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

msl-1^Hsp83.PC rescues msl-1^γ269

(Chang and Kuroda, 1998)

Comments

Rescues the recessive lethality of msl-1^γ269 males.

(Chang and Kuroda, 1998)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

msl-1^Hsp83.PC

(Dahlsveen et al., 2006)

Name Synonyms

Heat shock protein 83 promoter construct of Chang

(Chang and Kuroda, 1998)

Secondary FlyBase IDs

References (7)

Report Sections

Open Close