FlyBase Allele Report: Dmel\lncRNA:roX1[SMC17A]

General Information

Symbol

Dmel\lncRNA:roX1^SMC17A

Species

D. melanogaster

Name

FlyBase ID

FBal0192997

Feature type

allele

Associated gene

Dmel\lncRNA:roX1

Associated Insertion(s)

P{3'}lncRNA:roX1^SMC17A

Carried in Construct

Also Known As

roX1^SMC17A

Key Links

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Deng and Meller, 2009)

Mutagen

P-element activity

(Apte et al., 2014, Deng et al., 2005)

gene conversion

(Apte et al., 2014)

Progenitor genotype

lncRNA:roX1^Δ891

(Apte et al., 2014)

Associated Insertion(s)

P{3'}lncRNA:roX1^SMC17A

Cytology

Description

Mobilisation of the P{roX1-lacZ.R}roX1^w+tandem insertion present in roX1^Δ891, resulting in the loss of 1.2kb of roX1 sequence flanking the P{roX1-lacZ.R}roX1^w+tandem insertion site and its replacement with more than 3kb of Ecol\lacZ sequence fused to the roX1 promoter. This arrangement appears to be the product of a gene conversion event that occurred when the double-stranded break produced by P-element mobilisation underwent gap repair using a sister chromatid template, and is consistent with repair driven by homology between the roX1 promoter on the broken chromosome more than 1kb from the break site and the roX1 promoter in the P{roX1-lacZ.R} element on the sister chromatid. An isolated 3' P-element end is present downstream of the Ecol\lacZ sequence (this is the result of the 5' P-element end present in the progenitor chromosome being precisely replaced by the 3' end during the gene conversion event).

(Apte et al., 2014)

Complex rearrangement resulting from gene conversion induced by P-element mobilisation. Base pairs 204-2362 of roX1 have been replaced by a full length Ecol\lacZ gene, the 'SV40' poly adenylation signal and a 3' P-element end.

(Deng et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable

(Deng et al., 2005)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Df(1)roX2Δ, lncRNA:roX1^SMC17A has lethal | male phenotype, suppressible by Dp(1;3)DC067

(Ramírez et al., 2015)

Df(1)roX2Δ, lncRNA:roX1^SMC17A has lethal | male phenotype, suppressible by Scer\GAL4^da.PU/lncRNA:roX2^UAS.cRa

(Ramírez et al., 2015)

Df(1)roX2Δ, lncRNA:roX1^SMC17A has lethal | male phenotype, suppressible by Dp(1;3)DC511

(Ramírez et al., 2015)

Df(1)roX2Δ, lncRNA:roX1^SMC17A has lethal | male phenotype, suppressible by Scer\GAL4^da.PU/lncRNA:roX1^UAS.cRa

(Ramírez et al., 2015)

Df(1)52, Polr2A^+tcos4Δ4.3, lncRNA:roX1^SMC17A has lethal | male | pupal stage phenotype, suppressible | partially by msl-2^Hsp83.PK/msl-1^Hsp83.PC

(Deng et al., 2005)

Enhancer of

Statement

Reference

Df(1)52, roX1[+], lncRNA:roX1^SMC17A, Polr2A^+tcos4Δ4.3, + is an enhancer of partially lethal - majority die | male phenotype of msl-1^Hsp83.PC, msl-2^Hsp83.PK

(Deng et al., 2005)

Other

Statement

Reference

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^SMC17A has lethal | male phenotype

(Deng and Meller, 2009)

Df(1)52, Polr2A^+tcos4Δ4.3, lncRNA:roX1^SMC17A has lethal | male | pupal stage phenotype

(Deng et al., 2005)

Df(1)52, Polr2A^+tcos4Δ4.3, lncRNA:roX1^SMC17A has partially lethal - majority die | male | larval stage phenotype

(Deng et al., 2005)

Df(1)52, Polr2A^+tcos4Δ4.3, lncRNA:roX1^SMC17A has abnormal developmental rate | male | larval stage phenotype

(Deng et al., 2005)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The lethality of roX1^SMC17A;Df(1)roX2Δ double mutant hemizygous males can be rescued by expression of an autosomal insertion of either roX1^Scer\UAS.cRa or roX2^Scer\UAS.cRa driven by Scer\GAL4^da.PU or by combination with either Dp(1;3)DC067 or Dp(1;3)DC511 (autosomal translocations of fragments from the X chromosome containing roX1 or roX2 gene, respectively).

(Ramírez et al., 2015)

Only 0.2% of roX1^SMC17A Df(1)52 hemizygous males with P{w⁺4Δ4.3} survive to adulthood and these typically die upon eclosion. Only 37% of these animals reach 3rd instar and those that do reach this stage 4-5 days late. Survival to adulthood is partially rescued (to 2.5%) by msl-1^Hsp83.PC; msl-2^Hsp83.PK.

(Deng et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

lncRNA:roX1^SMC17A is rescued by Scer\GAL4^da.PU/lncRNA:roX1^UAS.cRa

(Ramírez et al., 2015)

Comments

The lethality of roX1^SMC17A;Df(1)roX2Δ double mutant hemizygous males is rescued by expression of roX1^Scer\UAS.cRa under the expression of Scer\GAL4^da.PU.

(Ramírez et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

lncRNA:roX1^SMC17A

roX1^SMC17A

(Aleman et al., 2021, Valsecchi et al., 2021, Ramírez et al., 2015, Apte et al., 2014, Ilik et al., 2013, Deng and Meller, 2009, Deng and Meller, 2006, Deng et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (8)

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