FlyBase Allele Report: Dmel\lncRNA:roX1[ex6]

General Information

Symbol

Dmel\lncRNA:roX1^ex6

Species

D. melanogaster

Name

FlyBase ID

FBal0099841

Feature type

allele

Associated gene

Dmel\lncRNA:roX1

Associated Insertion(s)

Carried in Construct

Also Known As

roX1^ex6

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Deng et al., 2005, Kelley et al., 1999)

Progenitor genotype

lncRNA:roX1^MB710

(Deng et al., 2005, Kelley et al., 1999)

Cytology

Description

Deletion of nucleotides 25-1472.

(Deng and Meller, 2009)

1.4kb deletion of sequences within roX1 transcription unit, removing the intron and DNA either side.

(Kelley et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:3,861,216..3,862,663 [-]

Comment:

Deleted for bases 25 to 1472 where 1 is first base of GB:U85980.

(Deng and Meller, 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable

(Deng et al., 2005)

Phenotype Manifest In

Detailed Description

Statement

Reference

Normal male development is seen in roX1^MB710 animals.

(Meller and Rattner, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6, lncRNA:roX1^t4.9 has partially lethal | male phenotype, enhanceable by msl-1^L60/msl-1[+]

(Prabhakaran and Kelley, 2012)

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6, lncRNA:roX1^t4.9 has partially lethal | male phenotype, enhanceable by Spt5^Q314X/Spt5[+]

(Prabhakaran and Kelley, 2012)

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6, lncRNA:roX1^t4.9 has partially lethal | male phenotype, enhanceable by Spt5[+]/Spt5^E471Z

(Prabhakaran and Kelley, 2012)

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6, lncRNA:roX1^t4.9 has partially lethal | male phenotype, enhanceable by Spt5[+]/Spt5^A680Z

(Prabhakaran and Kelley, 2012)

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6, lncRNA:roX1^t4.9 has partially lethal | male phenotype, enhanceable by Spt5^S14F/Spt5[+]

(Prabhakaran and Kelley, 2012)

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6, lncRNA:roX1^t4.9 has partially lethal | male phenotype, enhanceable by Su(Tpl)[+]/Su(Tpl)^S-192

(Prabhakaran and Kelley, 2012)

lncRNA:roX1^ex6 has lethal phenotype, enhanceable by msl-2^Hsp83.PK/msl-1^Hsp83.PC

(Oh et al., 2003)

NOT Enhanced by

Statement

Reference

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6, lncRNA:roX1^t4.9 has partially lethal | male phenotype, non-enhanceable by JIL-1[+]/JIL-1^z2

(Prabhakaran and Kelley, 2012)

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6, lncRNA:roX1^t4.9 has partially lethal | male phenotype, non-enhanceable by Elongin-C[+]/EloC^G6035

(Prabhakaran and Kelley, 2012)

Suppressed by

Statement

Reference

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6, lncRNA:roX1^t4.9, msl-1^L60 has lethal | male phenotype, suppressible | partially by ocm^Q1297X/ocm[+]

(Lim and Kelley, 2013)

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6, lncRNA:roX1^t4.9, msl-1^L60 has lethal | male phenotype, suppressible | partially by ocm[+]/ocm^G1646E

(Lim and Kelley, 2013)

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6, lncRNA:roX1^t4.9, msl-1^L60 has lethal | male phenotype, suppressible | partially by ocm^S1590F/ocm[+]

(Lim and Kelley, 2013)

Df(1)52, Polr2A^+tcos4Δ4.3, lncRNA:roX1^ex6 has partially lethal - majority die | male phenotype, suppressible | partially by msl-2^Hsp83.PK/msl-1^Hsp83.PC

(Deng et al., 2005)

Df(1)52, lncRNA:roX1^ex6 has lethal phenotype, suppressible | partially by msl-2^Hsp83.PK/msl-1^Hsp83.PC

(Oh et al., 2003)

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6 has lethal | recessive | male limited phenotype, suppressible by lncRNA:roX2^Hsp83.PH

(Meller and Rattner, 2002)

NOT suppressed by

Statement

Reference

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6, lncRNA:roX1^t4.9 has partially lethal | male phenotype, non-suppressible by JIL-1[+]/JIL-1^z2

(Prabhakaran and Kelley, 2012)

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6, lncRNA:roX1^t4.9 has partially lethal | male phenotype, non-suppressible by Elongin-C[+]/EloC^G6035

(Prabhakaran and Kelley, 2012)

Enhancer of

Statement

Reference

roX1[+]/lncRNA:roX1^ex6 is an enhancer of partially lethal - majority die | dominant | female phenotype of sc^sisB-1, sisA[+]/sisA¹

(Gladstein et al., 2010)

Df(1)52, roX1[+], lncRNA:roX1^ex6, Polr2A^+tcos4Δ4.3, + is an enhancer of partially lethal - majority die | male phenotype of msl-1^Hsp83.PC, msl-2^Hsp83.PK

(Deng et al., 2005)

NOT Enhancer of

Statement

Reference

Df(1)52/lncRNA:roX1^ex6 is a non-enhancer of abnormal eye color | male phenotype of w^B1-12

(Park et al., 2002)

Df(1)52/lncRNA:roX1^ex6 is a non-enhancer of abnormal eye color | female phenotype of w^69C

(Park et al., 2002)

Suppressor of

Statement

Reference

Df(1)52, lncRNA:roX1^ex6, P{w⁺4Δ4.3} is a suppressor of lethal | female | heat sensitive phenotype of msl-2^Hsp83.PK

(Lim and Kelley, 2012)

Df(1)52, roX1[+], lncRNA:roX1^ex6, + is a suppressor | partially of lethal | female limited phenotype of msl-1^Hsp83.PC, msl-2^Hsp83.PK

(Deng et al., 2005)

lncRNA:roX1^ex6 is a suppressor of partially lethal phenotype of Df(1)52, Polr2A^+tcos4Δ4.3, msl-2^Hsp83.PK

(Meller and Rattner, 2002)

lncRNA:roX1^ex6 is a suppressor of abnormal developmental rate | female phenotype of msl-2^Hsp83.PK

(Meller and Rattner, 2002)

lncRNA:roX1^ex6 is a suppressor of partially lethal phenotype of msl-2^Hsp83.PK

(Meller and Rattner, 2002)

lncRNA:roX1^ex6 is a suppressor of abnormal developmental rate | female phenotype of Df(1)52, P{w⁺4Δ4.3}, msl-2^Hsp83.PK

(Meller and Rattner, 2002)

Df(1)52/lncRNA:roX1^ex6 is a suppressor of abnormal eye color | male phenotype of w^69C

(Park et al., 2002)

NOT Suppressor of

Statement

Reference

lncRNA:roX1^ex6 is a non-suppressor of lethal | female | heat sensitive phenotype of msl-2^Hsp83.PK

(Lim and Kelley, 2012)

Df(1)52/lncRNA:roX1^ex6 is a non-suppressor of abnormal eye color | female phenotype of w^69C

(Park et al., 2002)

Df(1)52/lncRNA:roX1^ex6 is a non-suppressor of abnormal eye color | male phenotype of w^B1-12

(Park et al., 2002)

Other

Statement

Reference

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6, lncRNA:roX1^t4.9, msl-1^L60 has lethal | male phenotype

(Lim and Kelley, 2013)

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6, lncRNA:roX1^t4.9, msl-1^L60, ocm^Q1297X/ocm[+] has partially lethal | male phenotype

(Lim and Kelley, 2013)

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6, lncRNA:roX1^t4.9, msl-1^L60, ocm[+]/ocm^G1646E has partially lethal | male phenotype

(Lim and Kelley, 2013)

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6, lncRNA:roX1^t4.9, msl-1^L60, ocm^S1590F/ocm[+] has partially lethal | male phenotype

(Lim and Kelley, 2013)

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6, lncRNA:roX1^t4.9 has partially lethal | male phenotype

(Lim and Kelley, 2013, Prabhakaran and Kelley, 2012)

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6, lncRNA:roX1^t4.9, msl-1^L60/msl-1[+] has lethal | male phenotype

(Prabhakaran and Kelley, 2012)

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6 has lethal | male phenotype

(Deng and Meller, 2009)

Df(1)52, Polr2A^+tcos4Δ4.3, lncRNA:roX1^ex6 has partially lethal - majority die | male phenotype

(Deng et al., 2005)

Df(1)52, lncRNA:roX1^ex6 has lethal phenotype

(Oh et al., 2003)

Df(1)52, lncRNA:roX1^ex6 has lethal | male phenotype

(Park et al., 2003)

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6 has lethal | male limited phenotype

(Stuckenholz et al., 2003)

Df(1)52, P{w⁺4Δ4.3}, lncRNA:roX1^ex6 has lethal | recessive | male limited phenotype

(Meller and Rattner, 2002)

Df(1)52, Polr2A^+tcos4Δ4.3, lncRNA:roX1^ex6 has lethal phenotype

(Park et al., 2002)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Df(1)52/lncRNA:roX1^ex6 is a non-enhancer of pigment cell | male phenotype of w^B1-12

(Park et al., 2002)

Df(1)52/lncRNA:roX1^ex6 is a non-enhancer of pigment cell | female phenotype of w^69C

(Park et al., 2002)

Suppressor of

Statement

Reference

lncRNA:roX1^ex6 is a suppressor | partially of polytene chromosome phenotype of E(bx)^Nurf301-3/Df(3L)3643

(Bai et al., 2007)

Df(1)52/lncRNA:roX1^ex6 is a suppressor of pigment cell | male phenotype of w^69C

(Park et al., 2002)

NOT Suppressor of

Statement

Reference

Df(1)52/lncRNA:roX1^ex6 is a non-suppressor of pigment cell | female phenotype of w^69C

(Park et al., 2002)

Df(1)52/lncRNA:roX1^ex6 is a non-suppressor of pigment cell | male phenotype of w^B1-12

(Park et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

One paternally-derived copy of roX1^ex6 reduces the viability of sc^sisB-1 sisA¹/+ females.

(Gladstein et al., 2010)

The puffing of the male X polytene chromosome that is seen in E(bx)^Nurf301-3 mutants is regionally inhibited if they are also mutant for roX1 (carrying roX1^ex6); in this case, puffing of a large portion of the distal X, including the region around the roX1 locus, is strongly reduced, while the rest of the chromosome still displays a puffed morphology.

Chromosomal decondensation surrounding autosomal insertions of P{GMroX2} is seen in polytene chromosomes if E(bx)^Nurf301-3 is introduced into a roX1^- roX2^- background (roX1^ex6 Df(1)52 P{w⁺4Δ4.3}) carrying P{GMroX2} insertions.

(Bai et al., 2007)

Only 5% of roX1^ex6 Df(1)52 hemizygous males with P{w⁺4Δ4.3} survive to adulthood, but these animals are abundant and healthy at late third instar. Survival to adulthood is partially rescued (to 11.9%) by msl-1^Hsp83.PC; msl-2^Hsp83.PK.

(Deng et al., 2005)

The addition of msl-1^Hsp83.PC and msl-2^Hsp83.PK to roX1^ex6 males reduces their viability. roX1^ex6, Df(1)52 males have a very low viability, only up to 6.6% of males survive. This loss of viability is partially rescued by adding msl-1^Hsp83.PC and msl-2^Hsp83.PK, with up to 45% of males surviving.

(Oh et al., 2003)

The majority of roX1^ex6; Df(1)52 double homozygous males carrying P{w⁺4Δ4.3} do not survive to adulthood. This male lethality is almost completely rescued by roX2^ΔDHS.Hsp83.

(Park et al., 2003)

roX1^ex6 Df(1)52/Y males carrying P{w⁺4Δ4.3} generally do not survive to adulthood. This lethality is substantially rescued by an autosomal copy of roX1^Hsp83.PM, roX1^Δ1.Hsp83, roX1^Δ2.Hsp83, roX1^Δ3.Hsp83, roX1^Δ4.Hsp83, roX1^Δ5.Hsp83, roX1^Δ6.Hsp83, roX1^Δ7.Hsp83, roX1^Δ8.Hsp83, roX1^Δ9.Hsp83, roX1^Δ30.Hsp83, roX1^L.Hsp83 or roX1^SL.Hsp83. roX1^ex6 Df(1)52/Y males carrying P{w⁺4Δ4.3} generally do not survive to adulthood. This lethality is weakly rescued by an autosomal copy of roX1^Δ11.Hsp83. roX1^ex6 Df(1)52/Y males carrying P{w⁺4Δ4.3} generally do not survive to adulthood. This lethality is not substantially rescued by an autosomal copy of roX1^a.Hsp83, roX1^Hsp83.5', roX1^Hsp83.3' or roX1^Δ10.Hsp83. roX1^ex6 Df(1)52/Y males carrying P{w⁺4Δ4.3} generally do not survive to adulthood. This lethality is partly rescued by an autosomal copy of roX1^ΔO.Hsp83, roX1^ΔIR.Hsp83, roX1^5'S.Hsp83 or roX1^3'S.Hsp83.

(Stuckenholz et al., 2003)

Males carrying roX1^ex6,Df(1)52/Y ; P{w⁺4Δ4.3} exhibit only 5% survival. Df(1)52 deletes roX2 amongst other genes, P{w⁺4Δ4.3} is a cosmid that contains most of the genes deleted by Df(1)52, but not roX2. Females homozygous for roX1^ex6,Df(1)52 and also carrying P{w⁺4Δ4.3} are viable. The addition of roX2^Hsp83.PH to males carrying roX1^ex6,Df(1)52/Y ; P{w⁺4Δ4.3} suppresses the lethality seen in those animals.

(Meller and Rattner, 2002)

Eye colour: the expression of w^69C in females carrying w^69C in a w^- background is unchanged if they are also carrying roX1^ex6 Df(1)52 P{w⁺4Δ4.3}. Eye colour: males carrying w^69C in a w^- background that are also carrying roX1^ex6 Df(1)52 P{w⁺4Δ4.3} show almost complete derepression of w^69C expression in the ventral part of the eye. Eye colour: the expression of w^B1-12 in males carrying w^B1-12 in a w^- background is unchanged if they are also carrying roX1^ex6 Df(1)52 P{w⁺4Δ4.3} (these are escaper males). About 99% of roX1^ex6 Df(1)52 P{w⁺4Δ4.3} males die.

(Park et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

lncRNA:roX1^ex6 is rescued by lncRNA:roX1^Hsp83.PM

(Meller and Rattner, 2002)

Partially rescued by

Df(1)52/lncRNA:roX1^ex6 is partially rescued by lncRNA:roX1^t4.9

(Prabhakaran and Kelley, 2010)

Not rescued by

lncRNA:roX1^ex6 is not rescued by lncRNA:roX1^Hsp83.3'

(Meller and Rattner, 2002)

lncRNA:roX1^ex6 is not rescued by lncRNA:roX1^Hsp83.5'

(Meller and Rattner, 2002)

Comments

P{GMroX1}75C (which carries roX1^t4.9) partially rescues the lethality of roX1^ex6/Df(1)52 males.

(Prabhakaran and Kelley, 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

lncRNA:roX1^ex6

roX1^ex6

(Kim et al., 2018, Apte et al., 2014, Figueiredo et al., 2014, Lim and Kelley, 2013, Lundberg et al., 2013, Lim and Kelley, 2012, Prabhakaran and Kelley, 2012, Gladstein et al., 2010, Prabhakaran and Kelley, 2010, Deng and Meller, 2009, Kelley et al., 2008, Bai et al., 2007, Larschan et al., 2007, Larschan et al., 2007, Park et al., 2007, Deng and Meller, 2006, Deng et al., 2005, Park et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (30)

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