The addition of msl-2^Hsp83.PK leads to males with completely female genitalia and forelegs, yet nearly normal abdomens. The addition of one copy of P{snf⁺,dhd⁺} to Sxl^Mf1/Y males reduces the viability of males to about 50% of the expected number. The addition of two copies reduces the survival rate to about 3%. The survivors exhibit a wide range of imaginal discs defects, including thin or missing bristles, as well as small and rough eyes. Also feminizing effects are also seen in the sex combs, the majority of flies show some loss of sex combs, and only 1% had wild-type male sex combs. Intersexuality is predominantly of the "mosaic" type in which individual cells are either male or female rather than intermediate. The addition of noi^+t3.6 to Sxl^Mf1/Y males with two copies of P{snf⁺,dhd⁺} has no significant effect on the foreleg feminisation phenotype seen in those flies. There is a slight increase in the foreleg feminisation phenotype seen in Sxl^Mf1/Y males carrying two copies of P{snf⁺,dhd⁺}, when you compare the addition of homozygous snRNP70K⁶², to heterozygous snRNP70K⁶². The removal of noi function (by adding noi²/noi^D) leads to an almost complete suppression of the foreleg feminisation phenotype seen in Sxl^Mf1/Y flies with two copies of P{snf⁺,dhd⁺}. The addition of two copies of P{snf⁺,dhd⁺} to Sxl^Mf1/Y males that also carry noi²/noi^D, leads to feminisation of the foreleg and a reduction in viability. Sxl^Mf1/Y males with a single extra copy of P{snf⁺,dhd⁺}, are considerably more masculine after the addition of Df(1)J210.

The addition of snRNP70K::Hsap\SFRS1^T:Zzzz\FLAG to Sxl^Mf1/Y males has no effect on the foreleg feminisation phenotype seen in those flies.