FlyBase Allele Report: Dmel\vir[2f]

General Information

Symbol

Dmel\vir^2f

Species

D. melanogaster

Name

FlyBase ID

FBal0040111

Feature type

allele

Associated gene

Dmel\vir

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Hilfiker et al., 1995)

Progenitor genotype

Cytology

Description

Amino acid replacement: M1283K.

(Niessen et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:23,362,230..23,362,230 [-]

Nucleotide change:

T23362230A

Amino acid change:

M1283K | vir-PA

Reported amino acid change:

M1283K

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Niessen et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

vir^2f homozygous females are completely lethal.

(Knuckles et al., 2018)

vir^2f homozygotes are female lethal.

(Haussmann et al., 2016)

Female-specific allele.

(Niessen et al., 2001)

X chromosomal recombination frequencies in female germ cells homozygous for vir^2f are only slightly reduced compared to wild-type.

(Bopp et al., 1999)

Female germ cells homozygous for vir^2f can complete oogenesis. Females carrying these homozygous germ line clones give rise to male and female offspring in a 1:1 ratio when mated to vir⁺ males and give rise to purely male offspring when mated to homozygous vir^2f or vir^ts males. These phenotypes are rescued if the homozygous germ line clones are induced in females carrying vir^+t10. Females carrying vir⁶ homozygous germ line clones give rise only to male offspring when mated to vir^2f males, and only a few percent of these males survive to adulthood.

(Schutt et al., 1998)

Homozygotes and transheterozygotes with other EMS induced vir mutations exhibit normal male viability and fertility. Transheterozygotes with vir^ts transform XX animals into intersexes at 29^oC. Homozygous clones that occur in the sexually dimorphic regions of females differentiate male structures. Clones in males produce normal male structures. Foreleg and genital vir^2f/vir⁶ discs transplanted into vir⁺ hosts differentiate poorly developed male structures. Sxl⁺/Sxl⁺;vir^2f/vir^ts and Sxl^f1/Sxl⁺;vir^2f/vir⁺ are viable but doubly heterozygous Sxl^f1/Sxl⁺;vir^2f/vir^ts females are lethal.

(Hilfiker et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

vir^2f has lethal | female phenotype, suppressible by Flacc^C/nito[+]/nito¹/Flacc[+]

(Knuckles et al., 2018)

vir^2f has lethal | recessive | female phenotype, suppressible by Mettl3^Delta22-3/Ime4[+]

(Haussmann et al., 2016)

vir^2f has lethal | female | larval stage phenotype, suppressible by msl-2^γ136

(Hilfiker et al., 1995)

vir^2f has lethal | female | larval stage phenotype, suppressible by mle⁹

(Hilfiker et al., 1995)

vir^unspecified/vir^2f has lethal | female | larval stage phenotype, suppressible by Sxl^M1

(Hilfiker et al., 1995)

vir^unspecified/vir^2f has lethal | female | larval stage phenotype, suppressible by Sxl^M4

(Hilfiker et al., 1995)

vir^unspecified/vir^2f has lethal | female | larval stage phenotype, suppressible by Sxl^F1.hs

(Hilfiker et al., 1995)

vir^2f has lethal | female | larval stage phenotype, suppressible by msl-1^γ222

(Hilfiker et al., 1995)

NOT suppressed by

Statement

Reference

vir^2f has lethal | female phenotype, non-suppressible by nito[+]/nito¹

(Knuckles et al., 2018)

vir^2f has lethal | female phenotype, non-suppressible by Flacc^C/Flacc[+]

(Knuckles et al., 2018)

Enhancer of

Statement

Reference

vir^2f is an enhancer of visible phenotype of Sxl^Mf1, snf^+t5.5

(Cline et al., 1999)

Suppressor of

Statement

Reference

vir^2f/vir²² is a suppressor of lethal | male phenotype of Sxl^M1

(Niessen et al., 2001)

vir²³/vir^2f is a suppressor of lethal | male phenotype of Sxl^M1

(Niessen et al., 2001)

vir^2f/Df(2R)vir¹⁵ is a suppressor of lethal | male phenotype of Sxl^M1

(Niessen et al., 2001)

vir^2f is a suppressor of lethal phenotype of Sxl^M12, snf^+t5.5

(Cline et al., 1999)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The complete lethality of vir^2f homozygous females cannot be suppressed by combination with a single copy of nito¹ or Flacc^C individually but heterozygosity for both does partially restore viability of the vir^2f/vir^2f females.

(Knuckles et al., 2018)

The female lethality characteristic for vir^2f homozygotes is rescued to a high degree by combination with a single copy of Ime4^Δ22-3.

(Haussmann et al., 2016)

The addition of homozygous vir^2f suppresses the lethality seen in P{snf⁺,dhd⁺}, Sxl^M12/Y males.

(Cline et al., 1999)

Sxl^M4/+ ; vir⁶/vir^2f animals derived from females carrying homozygous vir⁶ germ line clones mated to vir^2f/+ males survive, but are sexually transformed. Sxl^M4/+ ; vir^2f/vir^2f animals derived from females carrying homozygous vir^2f germ line clones mated to homozygous vir^2f males survive, but are sexually transformed. Sxl^M1 cannot rescue the XX progeny of females carrying homozygous vir^2f germ line clones mated to homozygous vir^2f males whether it is paternally or maternally inherited.

(Schutt et al., 1998)

Female lethality can be rescued by msl-1^γ222, msl-2^γ136 or mle⁹ mutations, rescued animals are transformed into pseudomales. The presence of Sxl^M1, Sxl^M4 or Sxl^F1.hs rescues the female lethality of vir transheterozygotes, animals are strongly macsculinised and in some cases transformed to pseudomales.

(Hilfiker et al., 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

vir^2f is rescued by vir^+t10

(Niessen et al., 2001)

vir^2f is rescued by vir^+t10

(Schutt et al., 1998)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

77886

Dp(1;Y)Bar^S, Bar⁺; cn¹ bw¹ vir^2f/CyO

Notes on Origin

Discoverer

Comments

Homozygous larvae and heteroallelic larvae with vir³, reared at 25^oC, have msl-1 and mle associated with numerous sites along the paired X chromosomes.

(Hilfiker et al., 1994)

Protein is defective in functions required for dosage compensation and sex determination.

(Hilfiker et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

vir^2F

(Bawankar et al., 2021, Knuckles et al., 2018, Haussmann et al., 2016, Cline and Meyer, 1996, Horabin and Schedl, 1996)

vir^2f

Name Synonyms

Secondary FlyBase IDs

References (13)

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