Amino acids 85 to 263 are driven by The hsp70 promoter. The Tag:FLAG tag has been placed at the N terminus. Has an extra Alanine at the C terminus.
There is a complete loss of male viability in homozygous mutants where expression of msl-1FN.hs.T:Zzzz\FLAG in induced by heatshock.
msl-1FN.hs.Tag:FLAG has phenotype, suppressible by msl-2Hsp83.PK
The addition of msl-2Hsp83.PK suppresses the male viability phenotype seen in heatshocked msl-1FN.hs.T:Zzzz\FLAG flies.