FB2024_03 , released June 25, 2024
Allele: Dmel\e(y)34
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General Information
Symbol
Dmel\e(y)34
Species
D. melanogaster
Name
FlyBase ID
FBal0003372
Feature type
allele
Associated gene
Dmel\e(y)3
Associated Insertion(s)
Stalker{}e(y)34
Carried in Construct
Also Known As
e(y)3u1
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
Stalker-hybrid dysgenesis
(Georgiev and Gerasimova, 1989)
Progenitor genotype
Associated Insertion(s)
Stalker{}e(y)34
Cytology
Description

Insertion of a Stalker element into exon 10 at position 4956 from the start of the longest open reading frame. This generates a stop codon in the vicinity of the insertion site, which is predicted to generate a truncated protein lacking the last 349 amino acid residues (the PHD domains). Transcription is also dramatically lowered.

(Shidlovskii et al., 2005)

Insertion of Stalker element.

(Georgiev and Gerasimova, 1989)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      e(y)34 mutant embryos exhibit abnormal nuclear division synchronization, with delays in mitosis. This results in morphological disturbances at later stages, even to the degree of the absence of the head.

      Homo- or hemizygous e(y)34 mutants exhibit female sterility, decrease in viability, and disturbances in femur development in adults.

      (Vorobyeva et al., 2009)

      Homozygous females and hemizygous males are viable and display a 'bent-leg' phenotype with a penetrance of about 20%.

      (Chalkley et al., 2008)

      e(y)34 mutation decreases viability, by 50% in hemizygous males and by 20% in homozygous females. Weak disturbances in the development of the femur, a shortened body and expanded wings are also seen in 15 to 20% of flies. Mutant flies exhibit a repression of heterochromatin silencing.

      (Shidlovskii et al., 2005)

      Mutant flies have a shortened body and spread wings. Males show reduced viability and have defects in femur development.

      (Shidlovskii et al., 2005)

      Flies show slightly reduced viability.

      (Soldatov et al., 1999)

      No mutant phenotype in the wing.

      (Melnikova et al., 1996)

      Homozygotes display reduced viability.

      (Georgiev, 1994)

      Stable mutation. Homozygotes have reduced viability and thin bristles. Has no effect on the y+ phenotype.

      (Georgiev and Gerasimova, 1989)

      head and thorax bristles yellow; microchaetae yellow; thin bristles; female sterile; reduced viability.

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      e(y)34 is an enhancer of abnormal eye color phenotype of wsp2, zop6R1

      (Georgiev, 1994)

      e(y)34 is an enhancer of abnormal eye color phenotype of wsp4, zv77h

      (Georgiev, 1994)

      e(y)34 is an enhancer of abnormal eye color phenotype of wsp4, z1

      (Georgiev, 1994)

      e(y)34 is an enhancer of abnormal eye color phenotype of wsp4, zop6

      (Georgiev, 1994)

      e(y)34 is an enhancer of abnormal eye color phenotype of wsp4, zop6R1

      (Georgiev, 1994)

      e(y)34 is an enhancer of abnormal eye color phenotype of wsp1, zv77h

      (Georgiev, 1994)

      e(y)34 is an enhancer of abnormal eye color phenotype of wsp1, z1

      (Georgiev, 1994)

      e(y)34 is an enhancer of abnormal eye color phenotype of wsp1, zop6

      (Georgiev, 1994)

      e(y)34 is an enhancer of abnormal eye color phenotype of wsp1, zop6R1

      (Georgiev, 1994)

      e(y)34 is an enhancer of abnormal eye color phenotype of wsp2, zv77h

      (Georgiev, 1994)

      e(y)34 is an enhancer of abnormal eye color phenotype of wsp2, z1

      (Georgiev, 1994)

      e(y)34 is an enhancer of abnormal eye color phenotype of wsp2, zop6

      (Georgiev, 1994)
      Other
      Statement
      Reference

      brmI21/brm[+], e(y)34 has lethal phenotype

      (Chalkley et al., 2008)

      Taf91, e(y)34 has lethal phenotype

      (Soldatov et al., 1999, Georgiev, 1994)

      e(y)21, e(y)34 has lethal phenotype

      (Georgiev, 1994)
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      e(y)34 has leg phenotype, enhanceable by brm[+]/brm2

      (Chalkley et al., 2008)

      e(y)34 has leg phenotype, enhanceable by mor[+]/mor1

      (Chalkley et al., 2008)

      e(y)34 has leg phenotype, enhanceable by polybromo33.2/Bap170[+]/Bap170G5986/polybromo[+]

      (Chalkley et al., 2008)
      NOT Enhanced by
      Statement
      Reference

      e(y)34 has leg phenotype, non-enhanceable by osa[+]/osa308

      (Chalkley et al., 2008)

      e(y)34 has leg phenotype, non-enhanceable by osa00090/osa[+]

      (Chalkley et al., 2008)

      e(y)34 has leg phenotype, non-enhanceable by Snr101319/Snr1[+]

      (Chalkley et al., 2008)
      Enhancer of
      Statement
      Reference

      e(y)34 is an enhancer of phenotype of ctpN2s

      (Melnikova et al., 1996)

      e(y)34 is an enhancer of phenotype of ctpN1

      (Melnikova et al., 1996)

      e(y)34 is an enhancer of phenotype of ctn2s

      (Melnikova et al., 1996)

      e(y)34 is an enhancer of phenotype of ctpN30

      (Melnikova et al., 1996)

      e(y)34 is an enhancer of phenotype of z1

      (Georgiev, 1994)

      e(y)34 is an enhancer of phenotype of zop6R1

      (Georgiev, 1994)

      e(y)34 is an enhancer of phenotype of wsp4

      (Georgiev, 1994)

      e(y)34 is an enhancer of pigment cell phenotype of wsp4, zv77h

      (Georgiev, 1994)

      e(y)34 is an enhancer of pigment cell phenotype of wsp4, z1

      (Georgiev, 1994)

      e(y)34 is an enhancer of phenotype of wsp2

      (Georgiev, 1994)

      e(y)34 is an enhancer of pigment cell phenotype of wsp4, zop6

      (Georgiev, 1994)

      e(y)34 is an enhancer of pigment cell phenotype of wsp4, zop6R1

      (Georgiev, 1994)

      e(y)34 is an enhancer of phenotype of zop6

      (Georgiev, 1994)

      e(y)34 is an enhancer of phenotype of wsp1

      (Georgiev, 1994)

      e(y)34 is an enhancer of pigment cell phenotype of wsp1, zv77h

      (Georgiev, 1994)

      e(y)34 is an enhancer of pigment cell phenotype of wsp1, z1

      (Georgiev, 1994)

      e(y)34 is an enhancer of pigment cell phenotype of wsp2, zv77h

      (Georgiev, 1994)

      e(y)34 is an enhancer of pigment cell phenotype of wsp1, zop6

      (Georgiev, 1994)

      e(y)34 is an enhancer of pigment cell phenotype of wsp1, zop6R1

      (Georgiev, 1994)

      e(y)34 is an enhancer of phenotype of zv77h

      (Georgiev, 1994)

      e(y)34 is an enhancer of pigment cell phenotype of wsp2, z1

      (Georgiev, 1994)

      e(y)34 is an enhancer of pigment cell phenotype of wsp2, zop6

      (Georgiev, 1994)

      e(y)34 is an enhancer of pigment cell phenotype of wsp2, zop6R1

      (Georgiev, 1994)

      e(y)34 is an enhancer of phenotype of y2

      (Georgiev and Gerasimova, 1989)
      Suppressor of
      Statement
      Reference

      e(y)34, Bap170[+], Bap170G5986 is a suppressor of sex comb | ectopic phenotype of Pc1

      (Chalkley et al., 2008)

      e(y)34, Bap170[+], Bap170G5986 is a suppressor of sex comb | ectopic phenotype of Pc3

      (Chalkley et al., 2008)

      e(y)34 is a suppressor of phenotype of scD1

      (Gause et al., 1996)
      NOT Suppressor of
      Statement
      Reference

      e(y)34 is a non-suppressor of anterior notopleural bristle phenotype of sc+2MC, y+2MC

      (Gause et al., 1996)

      e(y)34 is a non-suppressor of anterior notopleural bristle phenotype of sc2#+9, y2#+9

      (Gause et al., 1996)
      Other
      Statement
      Reference

      e(y)34, y2 has microchaeta phenotype

      (Georgiev and Gerasimova, 1989)

      e(y)34, y2 has adult head bristle phenotype

      (Georgiev and Gerasimova, 1989)

      e(y)34, y2 has adult thorax & chaeta phenotype

      (Georgiev and Gerasimova, 1989)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The combination of e(y)34 with Hr46KG00850/+ or Hr46EY05451/+ causes a decrease in viability down to 24 and 8%, respectively.

      (Vorobyeva et al., 2011)

      brm2, mor1 and the combination of Bap170G5986/+ with polybromo33.2/+ act as strong dominant enhancers of the penetrance of the e(y)34/Y bent-leg phenotype in male flies. The combination of e(y)34/Y with brmI21/+ causes lethality.

      Hemizygous e(y)34 males display an almost complete suppression of the Pc1 and Pc3 leg transformation phenotype.

      (Chalkley et al., 2008)

      Lethal in combination with e(y)11. Viability is rescued by e(y)1+tSa and partially rescued by e(y)11.tSa.

      (Soldatov et al., 1999)

      Suppresses the scD1 phenotype slightly in males and strongly in females, including ocellar, post-vertical and scutellar bristles as well as the ANPs. Has no effect on ANP bristle formation caused by y+2MC, sc+2MC and y2#+9, sc2#+9. Has no effect on the Df(1)ase-1, sc5 or sc6 phenotype.

      (Gause et al., 1996)

      Enhances the cut wing phenotype of ctpN2s and ctn2s, ctpN1 and ctpN30. Acts additively in this enhancement with zv77h. In combination with e(y)34 and zv77h, mod(mdg4)ul enhances the mutant phenotype of ct6, though the gaps in the wing margin are still randomly distributed. No effect on mutant wing phenotype of ct6, ct2s or ct4s.

      (Melnikova et al., 1996)

      Heterozygotes with e(y)11 and e(y)21 are lethal. In z1 wzm strain e(y)34 causes a significant reduction of eye pigmentation.

      (Georgiev, 1994)

      Enhances the y2 phenotype; head and thorax bristles, and hairs are yellow. This enhancer effect is not suppressed by su(Hw)2.

      (Georgiev and Gerasimova, 1989)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      e(y)34 is rescued by e(y)3+tSa

      (Shidlovskii et al., 2005)

      e(y)34 is rescued by e(y)3Tag:FLAG

      (Shidlovskii et al., 2005)

      e(y)34 is rescued by e(y)3+tSa

      (Shidlovskii et al., 2005)
      Partially rescued by

      e(y)34 is partially rescued by e(y)3ΔPHD

      (Nikolenko et al., 2005)
      Comments

      e(y)3ΔPHD rescues female fertility and viability in the e(y)34 mutant strain and also suppresses the enhancement of the y2 and yinr phenotypes caused by e(y)34.

      (Nikolenko et al., 2005)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Mutation enhances the negative effect of z point mutations, zv77h, z1, zop6 or zop6R1, on w expression, in the presence of certain z alleles e(y)34 becomes dominant. e(y)34 also has a synergistic effect with zv77h on the expression of w (males have orange yellow eyes with red spots). w expression levels of wsp1, wsp2 and wsp4 are moderately reduced, exhibited by a reduced level of eye pigmentation, by e(y)34.

      (Georgiev, 1994)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (6)
      References (13)