FlyBase Allele Report: Dmel\Rok[2]

General Information

Symbol

Dmel\Rok²

Species

D. melanogaster

Name

FlyBase ID

FBal0122918

Feature type

allele

Associated gene

Dmel\Rok

Associated Insertion(s)

Carried in Construct

Also Known As

drok²

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Winter et al., 2001)

Progenitor genotype

Cytology

Description

G to A mutation in the conserved splice acceptor site at the 3' end of intron 1. The resulting transcript has a single base deletion of a G at the beginning of exon 2 and encodes only the first 21 amino acids of Rok followed by a 35 amino acid random peptide and a stop codon.

(Winter et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:16,632,914..16,632,914 [-]

Nucleotide change:

G16632914A

Reported nucleotide change:

G?A

Comment:

G to A nucleotide change at the splice acceptor of the first intron in the coding region. The resulting transcript has a single base deletion of a G at the beginning of exon 2 and encodes only the first 21 amino acids of Rok followed by a 35 amino acid random peptide and a stop codon.

(Winter et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal axis specification | embryonic stage 15 | male limited

(Okumura et al., 2010)

abnormal axis specification | embryonic stage 16 | male limited

(Okumura et al., 2010)

abnormal axis specification | embryonic stage 17 | male limited

(Okumura et al., 2010)

abnormal cell shape | germline clone

(Verdier et al., 2006)

abnormal cell shape | somatic clone

(Wang and Riechmann, 2007)

abnormal neuroanatomy | recessive | somatic clone

(Billuart et al., 2001)

abnormal planar polarity

(Simões et al., 2010)

abnormal planar polarity | recessive | somatic clone

(Winter et al., 2001)

abnormal planar polarity | somatic clone

(Capilla et al., 2012)

decreased fecundity | female | germline clone

(Verdier et al., 2006)

female sterile | germline clone

(Verdier et al., 2006)

lethal - all die before end of larval stage | recessive

(Winter et al., 2001)

some die during larval stage | recessive

(Winter et al., 2001)

visible | recessive | somatic clone

(Chung et al., 2007, Winter et al., 2001)

Phenotype Manifest In

adherens junction | somatic clone

(Warner and Longmore, 2009)

contractile ring | somatic clone

(Morais-de-Sá and Sunkel, 2013)

dorsal appendage | germline clone

(Verdier et al., 2006)

egg | germline clone

(Verdier et al., 2006)

egg chamber | somatic clone

(Wang and Riechmann, 2007)

embryonic/larval anterior midgut | embryonic stage 15 | male limited

(Okumura et al., 2010)

embryonic/larval anterior midgut | embryonic stage 16 | male limited

(Okumura et al., 2010)

embryonic/larval anterior midgut | embryonic stage 17 | male limited

(Okumura et al., 2010)

embryonic proventriculus | embryonic stage 15 | male limited

(Okumura et al., 2010)

embryonic proventriculus | embryonic stage 16 | male limited

(Okumura et al., 2010)

embryonic proventriculus | embryonic stage 17 | male limited

(Okumura et al., 2010)

epithelium | somatic clone

(Wang and Riechmann, 2007)

eye disc morphogenetic furrow | somatic clone

(Corrigall et al., 2007)

eye photoreceptor cell | somatic clone

(Winter et al., 2001)

eye photoreceptor cell | somatic clone | increased number

(Winter et al., 2001)

filamentous actin & nurse cell | germ-line clone

(Verdier et al., 2006)

filamentous actin & oocyte | germ-line clone

(Verdier et al., 2006)

follicle cell | somatic clone

(Wang and Riechmann, 2007)

hub cell | somatic clone

(Warner and Longmore, 2009)

mushroom body & axon | somatic clone

(Billuart et al., 2001)

nurse cell | germline clone

(Verdier et al., 2006)

nurse cell ring canal | germline clone

(Verdier et al., 2006)

ommatidium | somatic clone

(Capilla et al., 2012, Winter et al., 2001)

oocyte | germline clone

(Verdier et al., 2006)

plasma membrane & oocyte | germ-line clone

(Verdier et al., 2006)

presumptive embryonic salivary gland

(Xu et al., 2008)

wing hair | cell autonomous | somatic clone | increased number

(Winter et al., 2001)

wing hair | somatic clone | increased number

(Chung et al., 2007)

yolk granule | germline clone

(Verdier et al., 2006)

Detailed Description

Statement

Reference

Rho1^72O Rok² double zygotic mutants exhibit myoblast fusion defects. In contrast to the round and dense morphology seen in wild type, the F-actin enriched-structures between unfused fusion-competent myoblasts and miniature myotubes are irregularly shaped and exhibit abnormally long protrusions.

(Kim et al., 2015)

rok²/Y embryos do not exhibit significant gastrulation defects, compared to controls.

Heterozygosity for rok² does not results in any defects in leg morphogenesis.

(Manning et al., 2013)

The position of the contractile ring at the end of constriction is unbiased along the apical-basal axis in mutant clones in the follicle cells, in contrast to wild type. The midbody ring is always positioned to the apical side when the mutant cells regain their shape at the end of cell division, as occurs in wild type.

(Morais-de-Sá and Sunkel, 2013)

Homozygous clones result in defects in ommatidial rotation and in tissue morphology.

(Capilla et al., 2012)

rok² hemizygous embryos show laterality defects in the proventriculus and anterior midgut. The laterality of the other parts of the embryonic gut, including the hindgut and the posterior part of the midgut is normal in these mutants. The proventriculus and the anterior midgut do not rotate (as in wild-type) in these mutants at stages 15 to 17.

(Okumura et al., 2010)

baz and arm planar polarity is strongly defective in rok² mutants.

(Simões et al., 2010)

Overall length of the tracheal dorsal trunk is normal in mutant stage 16 embryos.

(Chung et al., 2009)

Single cell clones of rok² exhibit an increased apical cell area. Adherens junctions in multiple cell clones are intact.

(Warner and Longmore, 2009)

Salivary glands fail to invaginate or do not invaginate completely in 9% of homozygous embryos. Invagination often begins with anterior gland cells in the mutant embryos, instead of with dorsal-posterior cells as in wild type. 35% of mutant embryos show salivary gland migration defects, where the distal tip cells turn but the proximal half of the gland does not, and the gland fails to migrate posteriorly.

(Xu et al., 2008)

Homozygous clones in the wing result in a multiple wing hair phenotype.

(Chung et al., 2007)

Homozygous cells in the morphogenetic furrow (in clones that encompass the morphogenetic furrow) show a statistically significant impairment in apical constriction and apicobasal contraction.

(Corrigall et al., 2007)

rok² mutant follicle cell clones divide normally, form a monolayered follicular epithelium, and retain polarity. However, these cells fail to adopt a normal shape. As a consequence, the epithelium is flatter in these regions than it is in regions with rok activity. rok² mutant cells are also stretched compared to neighboring wild-type cells. Furthermore, egg chambers with large follicle cell clones develop abnormal shapes as the cyst bulges outwards in the area of the clones.

(Wang and Riechmann, 2007)

Females carrying rok² germline clones (GLCs) are sterile and lay a relatively small number of eggs, all of which are abnormally shaped. 100% of these eggs exhibit fused flat dorsal appendages and invariably fail to hatch. The eggs are abnormally small in 50% of cases and appear "deflated" in the remaining cases. Further analysis of these GLCs shows that several aspects of oogenesis are defective.

From stage 8 of oogenesis onwards, yolk granules in rok² GLC oocytes aberrantly accumulate and remain at the oocyte cortex, instead of moving throughout the ooplasm. At stage 11, nurse cell cytoplasmic "dumping" is defective in 97% of egg chamber GLCs with nurse cells largely retaining their cytoplasm. rok² egg chambers contain between one and four abnormally shaped canals, but no major difference in the overall number of ring canals.

Normal oocyte polarity is not established in rok² GLC oocytes, although microtubules appear to be well organized.

The actin cytoskeleton is disrupted in rok² GLC oocytes; subcortical F-actin is disorganized in most oocytes and is characterized by the presence of actin clumps within the oocyte and along the length of the oocyte cortex. Cortical F-actin exhibits a somewhat diffuse organization along the oocyte membrane. Additionally, the F-actin network is perturbed in nurse cells in 86% of rok² GLCs. Nurse cells lack functional F-actin filaments and contain nuclei of increased size that are not properly localized near the center of the cell.

Oocytes exhibit an altered plasma membrane in 82% of rok² GLCs. There are frequent deformations along localized regions of the cortical membrane, resulting in protrusions into the oocyte cytoplasm. A significant detachment of the oocyte membrane from the apical membrane of follicle cells can also be observed.

(Verdier et al., 2006)

Homozygous axons in clones in the mushroom body extend significantly further than heterozygous neurons in the same mushroom body.

(Billuart et al., 2001)

Mutants die before developing into wandering third instar larvae. Homozygous clones in the eye are of a similar size to their wild-type siblings. About 50% of ommatidia show an increase or decrease in photoreceptor numbers. Of those ommatidia with the correct number of photoreceptor cells, 60% are misrotated. Very few ommatidia show aberrant chirality. In homozygous clones in the wing, more than 70% of cells produce multiple wing hairs. Most hairs within the clone maintain a largely distal orientation. The majority of cells in wing clones generate more than one F-actin bundle during prehair initiation. The F-actin based prehairs always initiate at the cell periphery and in the majority of cases maintain a roughly distal orientation. These wing phenotypes are cell autonomous.

(Winter et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Drak^del/Drak^KO, rok[+]/Rok² has lethal phenotype, suppressible by flw^G0172/flw^G0172

(Neubueser and Hipfner, 2010)

Drak^del/Drak^KO, rok[+]/Rok² has lethal phenotype, suppressible by sqh^E20.E21

(Neubueser and Hipfner, 2010)

Rok², zip^Ebr has visible | dominant phenotype, suppressible by Mbs³

(Mizuno et al., 2002)

Rok² has lethal | recessive | larval stage phenotype, suppressible by sqh^E21.Tag:FLAG

(Winter et al., 2001)

Rok² has lethal | recessive | larval stage phenotype, suppressible by sqh^E20.E21

(Winter et al., 2001)

NOT suppressed by

Statement

Reference

Drak^del/Drak^KO, rok[+]/Rok² has lethal phenotype, non-suppressible by sqh^{A20.A21.Tag:FLAG}

(Neubueser and Hipfner, 2010)

Rok² has lethal | recessive | larval stage phenotype, non-suppressible by sqh^{A20.A21.Tag:FLAG}

(Winter et al., 2001)

Rok² has lethal | recessive | larval stage phenotype, non-suppressible by sqh^A21.Tag:FLAG

(Winter et al., 2001)

Enhancer of

Statement

Reference

Rok² is an enhancer of visible | adult stage phenotype of Hsap\TTR^{V30M.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Oliveira da Silva et al., 2020)

rok[+]/Rok² is an enhancer of visible phenotype of Scer\GAL4^hs.PB, tow^UAS.cCa

(Chung et al., 2007)

Rok² is an enhancer of visible | recessive phenotype of dsh¹

(Winter et al., 2001)

NOT Enhancer of

Statement

Reference

rok[+]/Rok² is a non-enhancer of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, nmo^UAS.cUa

(Mirkovic et al., 2011)

rok[+]/Rok² is a non-enhancer of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

rok[+]/Rok² is a non-enhancer of visible | recessive phenotype of Rab23⁵¹

(Pataki et al., 2010)

rok[+]/Rok² is a non-enhancer of abnormal cell polarity phenotype of Scer\GAL4^hs.2sev, shg^{dCR3h.UAS.sgGFP}

(Mirkovic and Mlodzik, 2006)

Suppressor of

Statement

Reference

Rok² is a suppressor | partially of increased mortality during development phenotype of CycE^{UAS.Tag:HA.Medsh}, Med^{RNAi.UAS.CycE-HA}, Ras85D^{G12D.UAS.p53sh}, Scer\GAL4^Ser.PU, p53^{RNAi.UAS.RasG12D}

(Sekiya et al., 2023)

Rok²/Rok[+] is a suppressor | partially of decreased size | adult stage phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

Rok²/Rok[+] is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

rok[+]/Rok² is a suppressor of some die during pharate adult stage phenotype of aurA^IM, sqh^E20.E21

(Moon and Matsuzaki, 2013)

rok[+]/Rok² is a suppressor of abnormal neuroanatomy phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

Rok² is a suppressor of lethal | recessive | larval stage phenotype of Moe^G0323

(Speck et al., 2003)

rok[+]/Rok² is a suppressor of visible | heat sensitive phenotype of fz^I.hs

(Winter et al., 2001)

NOT Suppressor of

Statement

Reference

Rok²/Rok² is a non-suppressor of majority die during embryonic stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

Rok² is a non-suppressor of majority die during embryonic stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

rok[+]/Rok² is a non-suppressor of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, nmo^UAS.cUa

(Mirkovic et al., 2011)

rok[+]/Rok² is a non-suppressor of visible phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

rok[+]/Rok² is a non-suppressor of visible | recessive phenotype of Rab23⁵¹

(Pataki et al., 2010)

rok[+]/Rok² is a non-suppressor of abnormal cell polarity phenotype of Scer\GAL4^hs.2sev, shg^{dCR3h.UAS.sgGFP}

(Mirkovic and Mlodzik, 2006)

Other

Statement

Reference

Drak^del, rok[+]/Rok² has lethal phenotype

(Neubueser and Hipfner, 2010)

Drak^del, Rok² has lethal | embryonic stage phenotype

(Neubueser and Hipfner, 2010)

Drak^del/Drak^KO, rok[+]/Rok² has lethal phenotype

(Neubueser and Hipfner, 2010)

Drak^del/Drak^KO, rok[+]/Rok² has increased cell death phenotype

(Neubueser and Hipfner, 2010)

Drak^KO, rok[+]/Rok² has lethal phenotype

(Neubueser and Hipfner, 2010)

Rho1^E3.10, rok[+]/Rok² has visible phenotype

(Bayer et al., 2003)

Rho1^J3.8/Rho1[+], Rok² has visible | dominant phenotype

(Bayer et al., 2003)

Rok², zip[+]/zip^Ebr has visible | dominant phenotype

(Bayer et al., 2003)

Df(3R)sbd105/+, Rok² has visible | dominant phenotype

(Bayer et al., 2003)

Rho1^12-6/Rho1[+], Rok² has visible phenotype

(Bayer et al., 2003)

Rok², Sb^63b/Sb[+] has visible phenotype

(Bayer et al., 2003)

Rok², Sb[+]/Sb⁷⁰ has visible phenotype

(Bayer et al., 2003)

Rok², Sb^sbd-1/Sb^sbd-201 has visible phenotype

(Bayer et al., 2003)

rok[+]/Rok², zip^Ebr has visible phenotype

(Bayer et al., 2003)

Rho1^J3.8, rok[+]/Rok² has visible phenotype

(Bayer et al., 2003)

rok[+]/Rok², Sb^sbd-1/Sb^sbd-201 has visible phenotype

(Bayer et al., 2003)

Rho1^12-6, rok[+]/Rok² has visible | dominant phenotype

(Bayer et al., 2003)

rok[+]/Rok², Sb^63b has visible | dominant phenotype

(Bayer et al., 2003)

rok[+]/Rok², Sb⁷⁰ has visible | dominant phenotype

(Bayer et al., 2003)

Rho1^E3.10/Rho1[+], Rok² has visible | dominant phenotype

(Bayer et al., 2003)

Rok², zip^Ebr has visible | dominant phenotype

(Mizuno et al., 2002)

rok[+]/Rok², zip^Ebr has visible | dominant phenotype

(Mizuno et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rok² has hub cell | somatic clone phenotype, enhanceable by dia^GD9442/Scer\GAL4^Act5C.PI

(Warner and Longmore, 2009)

Suppressed by

Statement

Reference

Rho1^72O, Rok² has myoblast phenotype, suppressible by sqh^E21.Tag:FLAG

(Kim et al., 2015)

Rok², zip^Ebr has wing phenotype, suppressible by Mbs³

(Mizuno et al., 2002)

Rok² has wing hair | increased number phenotype, suppressible by sqh^E20.E21

(Winter et al., 2001)

NOT suppressed by

Statement

Reference

Rho1^72O, Rok² has myoblast phenotype, non-suppressible by sqh^{A20.A21.Tag:FLAG}

(Kim et al., 2015)

Enhancer of

Statement

Reference

Rok² is an enhancer of eye phenotype of Hsap\TTR^{V30M.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Oliveira da Silva et al., 2020)

Rok²/Rok[+] is an enhancer of wing hair | increased number phenotype of Scer\GAL4^en.PU, cmb^{UAS.RA.Tag:FLAG}

(Fagan et al., 2014)

rok[+]/Rok² is an enhancer of wing hair | increased number phenotype of Scer\GAL4^hs.PB, tow^UAS.cCa

(Chung et al., 2007)

Rok² is an enhancer of wing hair | increased number phenotype of dsh¹

(Winter et al., 2001)

NOT Enhancer of

Statement

Reference

rok[+]/Rok² is a non-enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, nmo^UAS.cUa

(Mirkovic et al., 2011)

rok[+]/Rok² is a non-enhancer of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

rok[+]/Rok² is a non-enhancer of wing hair phenotype of Rab23⁵¹

(Pataki et al., 2010)

rok[+]/Rok² is a non-enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, shg^{dCR3h.UAS.sgGFP}

(Mirkovic and Mlodzik, 2006)

Rok²/Rok[+] is a non-enhancer of wing blade posterior compartment phenotype of Scer\GAL4^en-e16E, pbl^RNAi.UAS

(Shandala et al., 2004)

rok[+]/Rok² is a non-enhancer of wing hair | increased number phenotype of Scer\GAL4^en-e16E, pbl^RNAi.UAS

(Shandala et al., 2004)

Suppressor of

Statement

Reference

Rok²/Rok[+] is a suppressor | partially of wing phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

Rok² is a suppressor of epithelial cell | pupal stage | heat sensitive phenotype of Pten^JF01859, Scer\GAL4^en.PU, Scer\GAL80^ts.αTub84B

(Bardet et al., 2013)

Rok² is a suppressor of wing | pupal stage | heat sensitive phenotype of Pten^JF01859, Scer\GAL4^en.PU, Scer\GAL80^ts.αTub84B

(Bardet et al., 2013)

Rok² is a suppressor of cell junction | pupal stage | heat sensitive phenotype of Pten^JF01859, Scer\GAL4^en.PU, Scer\GAL80^ts.αTub84B

(Bardet et al., 2013)

rok[+]/Rok² is a suppressor | partially of eye phenotype of Scer\GAL4^arm.PS, aurA^IM, aurA^UAS.cWa

(Moon and Matsuzaki, 2013)

rok[+]/Rok² is a suppressor of adult mushroom body phenotype of LIMK1^UAS.Tag:HA, Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

rok[+]/Rok² is a suppressor of eye phenotype of Rac1^GMR.PN

(Tan et al., 2003)

rok[+]/Rok² is a suppressor of wing hair | increased number phenotype of fz^I.hs

(Winter et al., 2001)

NOT Suppressor of

Statement

Reference

Rok²/Rok² is a non-suppressor of embryo | dorsal closure stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

Rok²/Rok² is a non-suppressor of embryonic/first instar larval cuticle | embryonic stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

Rok² is a non-suppressor of embryo | dorsal closure stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

Rok² is a non-suppressor of embryonic/first instar larval cuticle | embryonic stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

rok[+]/Rok² is a non-suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, nmo^UAS.cUa

(Mirkovic et al., 2011)

rok[+]/Rok² is a non-suppressor of wing hair phenotype of Scer\GAL4^en-e16E, kermit^GS2053

(Djiane and Mlodzik, 2010)

rok[+]/Rok² is a non-suppressor of wing hair phenotype of Rab23⁵¹

(Pataki et al., 2010)

rok[+]/Rok² is a non-suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, shg^{dCR3h.UAS.sgGFP}

(Mirkovic and Mlodzik, 2006)

Other

Statement

Reference

Rho1^72O, Rok² has myoblast phenotype

(Kim et al., 2015)

Drak^del, rok[+]/Rok² has embryonic/larval tracheal system phenotype

(Neubueser and Hipfner, 2010)

Drak^del, Rok² has cephalopharyngeal skeleton phenotype

(Neubueser and Hipfner, 2010)

Drak^del, Rok² has embryonic head phenotype

(Neubueser and Hipfner, 2010)

Drak^del/Drak^KO, rok[+]/Rok² has wing disc phenotype

(Neubueser and Hipfner, 2010)

Drak^del/Drak^KO, rok[+]/Rok² has wing pouch phenotype

(Neubueser and Hipfner, 2010)

Rho1^E3.10/Rho1[+], Rok² has leg phenotype

(Bayer et al., 2003)

Rho1^J3.8/Rho1[+], Rok² has leg phenotype

(Bayer et al., 2003)

Rok², zip[+]/zip^Ebr has leg phenotype

(Bayer et al., 2003)

Df(3R)sbd105/+, Rok² has leg phenotype

(Bayer et al., 2003)

Rho1^E3.10, Rok² has leg phenotype

(Bayer et al., 2003)

Rok², Sb^sbd-1/Sb^sbd-201 has leg phenotype

(Bayer et al., 2003)

Rho1^12-6, Rok² has leg phenotype

(Bayer et al., 2003)

Rok², Sb^63b has leg phenotype

(Bayer et al., 2003)

Rok², Sb⁷⁰ has leg phenotype

(Bayer et al., 2003)

Rok², zip^Ebr has leg phenotype

(Bayer et al., 2003)

rok[+]/Rok², Sb^sbd-1/Sb^sbd-201 has leg phenotype

(Bayer et al., 2003)

Rho1^12-6, rok[+]/Rok² has leg phenotype

(Bayer et al., 2003)

Rho1^J3.8, Rok² has leg phenotype

(Bayer et al., 2003)

rok[+]/Rok², Sb^63b has leg phenotype

(Bayer et al., 2003)

rok[+]/Rok², Sb⁷⁰ has leg phenotype

(Bayer et al., 2003)

Rok², zip^Ebr has wing phenotype

(Mizuno et al., 2002)

rok[+]/Rok², zip^Ebr has wing phenotype

(Mizuno et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Rok²/Rok² or Rok² hemizygosity fails to suppress the embryonic lethality and dorsal closure defects seen in embryos expressing crb^Y10A in a crb^GX24w-/crb^11A22 background.

(Flores-Benitez and Knust, 2015)

Rho1^72O Rok² double zygotic mutants exhibit myoblast fusion defects.

Expression of sqh^{E21.T:Zzzz\FLAG} suppresses the myoblast fusion defect seen in Rho1^72O Rok² double mutant embryos.

Expression of sqh^{A20.A21.T:Zzzz\FLAG} does not suppress the myoblast fusion defect seen in Rho1^72O Rok² double mutant embryos.

(Kim et al., 2015)

Heterozygosity significantly enhances the multiple hair cell phenotype of Scer\GAL4^en.PU cmb^Scer\UAS.RA wings.

(Fagan et al., 2014)

rok² clones generated in Pten^JF01859-expressing tissue suppresses the effect of the expression of Pten^JF01859 under the control of Scer\GAL80^ts.αTub84B and Scer\GAL4^en.PU on cell packing, namely the excess of short junctions. Despite a slightly higher proportion of non-rearranging junctions (1.15-fold), the rearranging junctions neither produce an excess of short junctions nor fluctuate extensively, but lengthen to produce long cell junctions as observed in wild-type tissue. Furthermore, the packing and cell rearrangement dynamics in the double mutant tissue is similar to the one observed in rok² single mutant tissue.

(Bardet et al., 2013)

A rok²/+ heterozygous background suppresses the enhanced lethality of aur^WK females bearing one copy of sqh^E20.E21, and ameliorates the enhanced rough eye phenotype of these flies.

A rok²/+ heterozygous background suppresses the enhanced lethality of aur^IM females bearing one copy of sqh^E20.E21, and ameliorates the enhanced rough eye phenotype of these flies.

(Moon and Matsuzaki, 2013)

More than half of Drak^del rok²/Drak^del third instar larvae are missing random elements of the tracheal tree.

The wing discs of Drak^del rok²/Drak^KO third instar larvae are composed of unusually thin epithelial layers which are grossly distorted. The wing pouch region has folds which project basally out of the plane of the epithelium, and these distortions are accompanied by massive apoptosis.

100% of Drak^del rok² double homozygous embryos die before hatching. They show a fully penetrant head defect, which is characterized by an anterior hole in the embryonic cuticle and anterior malformations of the cephalopharyngeal skeleton.

(Neubueser and Hipfner, 2010)

rok² does not exhibit dominant genetic interaction with Rab23⁵¹.

(Pataki et al., 2010)

Expression of dia^GD9442 in rok² MARCM clones does not affect mature epithelial adherens junctions. An increase in apical area is found, compared to rok² clones.

(Warner and Longmore, 2009)

The multiple wing hair phenotype caused by expression of tow^Scer\UAS.cCa under the control of Scer\GAL4^hs.PB using heat shock at 24 hours after puparium formation is enhanced if the flies are also carrying one copy of rok².

(Chung et al., 2007)

A rok²/+ background does not affect the Scer\GAL4^hs.2sev>shg^{dCR3h.Scer\UAS.T:Avic\GFP-rs} ommatidial phenotype.

(Mirkovic and Mlodzik, 2006)

Neither the reduction in compartment size nor the percentage of cells with multiple wing hairs in pbl^{dsRNA.Scer\UAS}; Scer\GAL4^en-e16E flies is enhanced by rok²/+.

(Shandala et al., 2004)

rok² shows a strong interaction (at least 50% of double heterozygotes have at least one malformed leg) with the following mutations: Sb⁷⁰. rok² shows a moderate interaction (25-49% of double heterozygotes have at least one malformed leg) with the following mutations: Sb^63b. rok² shows a weak interaction (5-24% of double heterozygotes have at least one malformed leg) with the following mutations: Df(3R)sbd105 and Rho1^12-6. Sb^sbd-201/Sb^sbd-1 shows a weak interaction (5-24% of double mutants have at least one malformed leg) with the following mutations: rok²/+.

(Bayer et al., 2003)

The small, rough eye phenotype of Rac1^GMR.PN flies is partially suppressed by heterozygosity for rok².

(Tan et al., 2003)

Addition of rok² to dsh¹ mutants results in a 2.5-fold increase in the number of multiple wing hair cells compared to dsh¹ single mutants. 4% of rok²/Y ; sqh^E20.E21/+ flies survive to adulthood. The multiple wing hair phenotype caused by rok² is almost completely suppressed by sqh^E20.E21.

(Winter et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Rok¹

(Winter et al., 2001)

Rescued by

Rok² is rescued by Rok^αTub84B.PW

(Winter et al., 2001)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6666

y¹ w¹¹¹⁸ Rok² P{neoFRT}19A/FM7c

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Drok²

(Marchetti et al., 2014, Capilla et al., 2012, Gault et al., 2012, Pataki et al., 2010, Simões et al., 2010, Chung et al., 2009, Chung et al., 2007, Verdier et al., 2006, Speck et al., 2003, Tan et al., 2003, Billuart et al., 2001, Winter et al., 2001)

Rok²

(Sekiya et al., 2023, Hildebrand et al., 2021, Oliveira da Silva et al., 2020, Aranjuez et al., 2016, Kasza et al., 2014, Simões et al., 2014, Ng and Luo, 2004)

dROK²

(Mirkovic et al., 2011)

dRok²

(Thuveson et al., 2019, Mirkovic and Mlodzik, 2006)

drok²

(Moon and Matsuzaki, 2013, Jenny, 2011, Bayer et al., 2003)

rock²

(Coravos and Martin, 2016)

Name Synonyms

Secondary FlyBase IDs

References (50)

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