FB2024_03 , released June 25, 2024
Allele: Dmel\sqhA21.Tag:FLAG
Open Close
General Information
Symbol
Dmel\sqhA21.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0083733
Feature type
allele
Associated gene
Dmel\sqh
Associated Insertion(s)
Carried in Construct
P{sqh-A21}
Key Links
Transgenic product class
missense_variant
(Jordan and Karess, 1997)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Jordan and Karess, 1997)
Mutagen
in vitro construct
(Jordan and Karess, 1997)
Progenitor genotype
Carried in construct
P{sqh-A21}
Cytology
Description

Contains sqh, expressed under its own promoter and containing the amino acid replacement S21A, tagged with Tag:FLAG.

(Jordan and Karess, 1997)
Allele components
Component
Use(s)
Regulatory region(s)
sqh
Encoded product / tool
sqh
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      One copy of sqhA21.T:Zzzz\FLAG in a wild-type background has no effect on eye development.

      (Lee and Treisman, 2004)

      Approximately 10-20% of egg chambers in germline clones expressing sqhA21.T:Zzzz\FLAG apparently have a failure of cytokinesis during the fourth round of cystocyte division as they consist of 7 binuclear nurse cells and one binuclear oocyte. A fraction of eggs derived from germline clones expressing sqhA21.T:Zzzz\FLAG are deposited and have three or four dorsal appendages. The four appendages are found in two pairs separated by a variable fraction of the egg circumference. When three appendages are present, the central one often appears to be a fusion of two appendages. Eggs with three or four dorsal appendages can be fertilised and begin development. These embryos have posterior segmentation defects. Only 36% of these embryos show partial dorsalisation (reduced head skeleton and a narrowing of the denticle belts).

      (Roth et al., 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      sqhA21.Tag:FLAG is an enhancer of visible | recessive phenotype of dsh1

      (Winter et al., 2001)
      NOT Enhancer of
      Suppressor of
      Statement
      Reference

      sqhA21.Tag:FLAG is a suppressor of lethal | male phenotype of flw6

      (Vereshchagina et al., 2004)

      sqhA21.Tag:FLAG is a suppressor of lethal | male phenotype of flwG0172

      (Vereshchagina et al., 2004)
      NOT Suppressor of
      Statement
      Reference

      sqhA21.Tag:FLAG is a non-suppressor of abnormal neuroanatomy phenotype of RhoGAPp190RNAi.GAP.UAS, Scer\GAL4ey-OK107

      (Billuart et al., 2001)

      sqhA21.Tag:FLAG is a non-suppressor of lethal | recessive | larval stage phenotype of Rok2

      (Winter et al., 2001)
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      sqhA21.Tag:FLAG is an enhancer of eye disc | somatic clone phenotype of MbsT541

      (Lee and Treisman, 2004)

      sqhA21.Tag:FLAG is an enhancer of wing hair | increased number phenotype of dsh1

      (Winter et al., 2001)
      NOT Enhancer of
      Suppressor of
      Statement
      Reference

      sqhA21.Tag:FLAG is a suppressor of eye disc | somatic clone phenotype of MbsT541

      (Lee and Treisman, 2004)
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      MbsT541 photoreceptor clones are restored to their wild-type apical location in the eye disc when discs carry one copy of the constitutively inactive the sqhA21.T:Zzzz\FLAG transgene.

      (Lee and Treisman, 2004)

      The viability of both flwG0172 and flw6 mutants is much greater in a sqhA21.T:Zzzz\FLAG background than a sqhA20.T:Zzzz\FLAG background.

      (Vereshchagina et al., 2004)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      sqhA21.Tag:FLAG partially rescues sqhAX3

      (Jordan and Karess, 1997)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      sqhA21.T:FLAG
      sqhA21.T:Zzzz\FLAG
      sqhA21.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (6)