FlyBase Allele Report: Dmel\Drak[KO]

General Information

Symbol

Dmel\Drak^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0246419

Feature type

allele

Associated gene

Dmel\Drak

Associated Insertion(s)

TI{TI}Drak^KO

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

recombination

(Neubueser and Hipfner, 2010)

FLPase

(Neubueser and Hipfner, 2010)

I-SceI

(Neubueser and Hipfner, 2010)

ends-out gene targeting

(Neubueser and Hipfner, 2010)

Progenitor genotype

Associated Insertion(s)

TI{TI}Drak^KO

Cytology

Description

Drak exons 3-5 (encoding most of the kinase domain) have been replaced by a w^+mW.hs marker.

(Neubueser and Hipfner, 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Neubueser and Hipfner, 2010)

viable

(Neubueser and Hipfner, 2010)

viable (with Drak^del)

(Neubueser and Hipfner, 2010)

visible

(Neubueser and Hipfner, 2010)

Phenotype Manifest In

metathoracic leg

(Neubueser and Hipfner, 2010)

wing

(Neubueser and Hipfner, 2010)

Detailed Description

Statement

Reference

The wing area of homozygous flies is significantly smaller than that of controls, with the wings being specifically shortened in the proximal-distal axis. The mutant flies sometimes show shortening of the legs, usually restricted to the T3 leg; leg segments (primarily the femur and tibia) are shortened and abnormally bent.

Drak^del/Drak^KO adults show a reduction in wing area compared to controls and a partially penetrant phenotype of T3 leg defects.

(Neubueser and Hipfner, 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Drak^KO has visible phenotype, enhanceable by Rho1^72O/Rho1[+]

(Neubueser and Hipfner, 2010)

Drak^KO has visible phenotype, enhanceable by zip²/zip[+]

(Neubueser and Hipfner, 2010)

Drak^KO has visible phenotype, enhanceable by Sb^63b/Sb[+]

(Neubueser and Hipfner, 2010)

Suppressed by

Statement

Reference

Drak^del/Drak^KO, rok[+]/Rok² has lethal phenotype, suppressible by flw^G0172/flw^G0172

(Neubueser and Hipfner, 2010)

Drak^del/Drak^KO, rok[+]/Rok² has lethal phenotype, suppressible by sqh^E20.E21

(Neubueser and Hipfner, 2010)

Drak^KO has visible phenotype, suppressible by sqh^E20.E21

(Neubueser and Hipfner, 2010)

NOT suppressed by

Statement

Reference

Drak^del/Drak^KO, rok[+]/Rok² has lethal phenotype, non-suppressible by sqh^{A20.A21.Tag:FLAG}

(Neubueser and Hipfner, 2010)

Suppressor of

Statement

Reference

Drak^KO is a suppressor of lethal phenotype of flw^G0172

(Neubueser and Hipfner, 2010)

Other

Statement

Reference

Drak^del/Drak^KO, rok[+]/Rok² has lethal phenotype

(Neubueser and Hipfner, 2010)

Drak^del/Drak^KO, rok[+]/Rok² has increased cell death phenotype

(Neubueser and Hipfner, 2010)

Drak^KO, rok[+]/Rok² has lethal phenotype

(Neubueser and Hipfner, 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Drak^KO has wing phenotype, enhanceable by Rho1^72O/Rho1[+]

(Neubueser and Hipfner, 2010)

Drak^KO has wing phenotype, enhanceable by zip²/zip[+]

(Neubueser and Hipfner, 2010)

Drak^KO has wing phenotype, enhanceable by Sb^63b/Sb[+]

(Neubueser and Hipfner, 2010)

Suppressed by

Statement

Reference

Drak^KO has metathoracic leg phenotype, suppressible by flw^G0172

(Neubueser and Hipfner, 2010)

Drak^KO has wing phenotype, suppressible by sqh^E20.E21

(Neubueser and Hipfner, 2010)

Drak^KO has metathoracic leg phenotype, suppressible by sqh^E20.E21

(Neubueser and Hipfner, 2010)

NOT suppressed by

Statement

Reference

Drak^KO has metathoracic leg phenotype, non-suppressible by sqh^{A20.A21.Tag:FLAG}

(Neubueser and Hipfner, 2010)

Other

Statement

Reference

Drak^del/Drak^KO, rok[+]/Rok² has wing disc phenotype

(Neubueser and Hipfner, 2010)

Drak^del/Drak^KO, rok[+]/Rok² has wing pouch phenotype

(Neubueser and Hipfner, 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The shortening of the wing which is seen in Drak^KO flies is enhanced if the flies are also heterozygous for one of Rho1^72O, zip² or Sb^63b.

The wing discs of Drak^del rok²/Drak^KO third instar larvae are composed of unusually thin epithelial layers which are grossly distorted. The wing pouch region has folds which project basally out of the plane of the epithelium, and these distortions are accompanied by massive apoptosis.

The T3 leg defects seen in Drak^KO mutants are suppressed by flw^G0172.

Expression of sqh^E20.E21 suppresses the short wing and deformed T3 leg phenotypes of Drak^KO mutants. Expression of sqh^{A20.A21.T:Zzzz\FLAG} has no effect on the deformed T3 leg phenotype of Drak^KO mutants.

(Neubueser and Hipfner, 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Drak^KO

drak^KO

(Neubueser and Hipfner, 2010)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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