FlyBase Allele Report: Dmel\sqh[A20.A21.Tag:FLAG]

General Information

Symbol

Dmel\sqh^{A20.A21.Tag:FLAG}

Species

D. melanogaster

Name

FlyBase ID

FBal0083735

Feature type

allele

Associated gene

Dmel\sqh

Associated Insertion(s)

Carried in Construct

P{sqh-A20,A21}

Also Known As

sqhAA

Key Links

Transgenic product class

missense_variant

(Jordan and Karess, 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Jordan and Karess, 1997)

Mutagen

in vitro construct

(Jordan and Karess, 1997)

Progenitor genotype

Carried in construct

P{sqh-A20,A21}

Cytology

Description

Contains sqh, expressed under its own promoter and containing the amino acid replacements T20A and S21A, tagged with Tag:FLAG.

(Jordan and Karess, 1997)

Allele components

Component

Use(s)

Regulatory region(s)

sqh

Encoded product / tool

sqh

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

sqh^{A20.A21.Tag:FLAG} is a non-enhancer of lethal - all die during P-stage phenotype of aurA^ST

(Moon and Matsuzaki, 2013)

sqh^{A20.A21.Tag:FLAG} is a non-enhancer of some die during pharate adult stage phenotype of aurA^IM

(Moon and Matsuzaki, 2013)

sqh^{A20.A21.Tag:FLAG} is a non-enhancer of increased mortality during development phenotype of aurA^WK

(Moon and Matsuzaki, 2013)

sqh^{A20.A21.Tag:FLAG} is a non-enhancer of visible | recessive phenotype of ck⁰⁷¹³⁰

(Winter et al., 2001)

Suppressor of

Statement

Reference

sqh^{A20.A21.Tag:FLAG} is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of asp^E3

(Rujano et al., 2013)

sqh^{A20.A21.Tag:FLAG} is a suppressor of visible phenotype of MYPT-75D^F117A.UAS, Scer\GAL4^Bx-MS1096

(Vereshchagina et al., 2004)

sqh^{A20.A21.Tag:FLAG} is a suppressor of lethal | male phenotype of flw⁶

(Vereshchagina et al., 2004)

sqh^{A20.A21.Tag:FLAG} is a suppressor | partially of abnormal neuroanatomy phenotype of Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng

(Kim et al., 2002)

NOT Suppressor of

Statement

Reference

sqh^{A20.A21.Tag:FLAG} is a non-suppressor of some die during pharate adult stage phenotype of aurA^IM

(Moon and Matsuzaki, 2013)

sqh^{A20.A21.Tag:FLAG} is a non-suppressor of increased mortality during development phenotype of aurA^WK

(Moon and Matsuzaki, 2013)

sqh^{A20.A21.Tag:FLAG} is a non-suppressor of abnormal cell polarity phenotype of Sgt1^s2383

(Andersen et al., 2012)

sqh^{A20.A21.Tag:FLAG} is a non-suppressor of lethal phenotype of Drak^del/Drak^KO, rok[+]/Rok²

(Neubueser and Hipfner, 2010)

sqh^{A20.A21.Tag:FLAG} is a non-suppressor of visible phenotype of flw¹, puc^A251.1F3/puc[+]

(Kirchner et al., 2007)

sqh^{A20.A21.Tag:FLAG} is a non-suppressor of lethal | male phenotype of flw⁶

(Vereshchagina et al., 2004)

sqh^{A20.A21.Tag:FLAG} is a non-suppressor of lethal | male phenotype of flw^G0172

(Vereshchagina et al., 2004)

sqh^{A20.A21.Tag:FLAG} is a non-suppressor of lethal | recessive | larval stage phenotype of Rok²

(Winter et al., 2001)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

sqh^{A20.A21.Tag:FLAG} is a non-enhancer of wing hair | increased number phenotype of ck⁰⁷¹³⁰

(Winter et al., 2001)

Suppressor of

Statement

Reference

sqh^{A20.A21.Tag:FLAG} is a suppressor of embryonic/larval brain | third instar larval stage phenotype of asp^E3

(Rujano et al., 2013)

sqh^{A20.A21.Tag:FLAG} is a suppressor of wing phenotype of MYPT-75D^F117A.UAS, Scer\GAL4^Bx-MS1096

(Vereshchagina et al., 2004)

sqh^{A20.A21.Tag:FLAG} is a suppressor of eye phenotype of Rac1^GMR.PN

(Tan et al., 2003)

sqh^{A20.A21.Tag:FLAG} is a suppressor | partially of larval longitudinal connective phenotype of Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng

(Kim et al., 2002)

NOT Suppressor of

Statement

Reference

sqh^{A20.A21.Tag:FLAG} is a non-suppressor of myoblast phenotype of Rho1^72O, Rok²

(Kim et al., 2015)

sqh^{A20.A21.Tag:FLAG} is a non-suppressor of neuroblast phenotype of Sgt1^s2383

(Andersen et al., 2012)

sqh^{A20.A21.Tag:FLAG} is a non-suppressor of metathoracic leg phenotype of Drak^KO

(Neubueser and Hipfner, 2010)

sqh^{A20.A21.Tag:FLAG} is a non-suppressor of wing phenotype of flw¹, puc^A251.1F3/puc[+]

(Kirchner et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of sqh^{A20.A21.T:Zzzz\FLAG} does not suppress the myoblast fusion defect seen in Rho1^72O Rok² double mutant embryos.

(Kim et al., 2015)

Introducing one copy of sqh^{A20.A21.T:Zzzz\FLAG} into aur^ST, aur^IM, or aur^WK mutants does not affect lethality.

(Moon and Matsuzaki, 2013)

sqh^{A20.A21.T:Zzzz\FLAG} suppresses the neuroepithelial defects of asp^E3 larval brains.

(Rujano et al., 2013)

The presence of sqh^{A20.A21.T:Zzzz\FLAG} fails to suppress the Sgt1^s2383 neuroblast cortical polarity phenotype.

(Andersen et al., 2012)

Expression of sqh^{A20.A21.T:Zzzz\FLAG} has no effect on the deformed T3 leg phenotype of Drak^KO mutants.

(Neubueser and Hipfner, 2010)

The wing defects seen in flw¹/Y ; puc^A251.1F3/+ flies are not suppressed by sqh^{A20.A21.T:Zzzz\FLAG}.

(Kirchner et al., 2007)

The blistering and crumpling of wings in flies expressing MYPT-75D^{F117A.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096 is suppressed when flies also express sqh^{A20.A21.T:Zzzz\FLAG}.

(Vereshchagina et al., 2004)

The small, rough eye phenotype of Rac1^GMR.PN flies is partially suppressed by heterozygosity for sqh^{A20.A21.T:Zzzz\FLAG}.

(Tan et al., 2003)

Xenogenetic Interactions

Statement

Reference

The midline crossover phenotype caused expression of Ggal\MLCK^ct.Scer\UAS under the control of Scer\GAL4^ftz.ng is partially suppressed by sqh¹ sqh^{A20.A21.T:Zzzz\FLAG}.

(Kim et al., 2002)

Complementation and Rescue Data

Fails to rescue

sqh^{A20.A21.Tag:FLAG} fails to rescue sqh^AX3

(Jordan and Karess, 1997)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

25764

y¹ w^*; P{sqh-A20,A21}1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Sqh^A20/A21

(Neubueser and Hipfner, 2010)

Sqh^AA

(Neubueser and Hipfner, 2010)

sqh^20A21A

(Andersen et al., 2012)

sqh^{A20.A21.T:FLAG}

sqh^{A20.A21.T:Zzzz\FLAG}

sqh^{A20.A21.Tag:FLAG}

sqh^A20A21

(Tan et al., 2003)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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