FlyBase Allele Report: Dmel\Sgt1[s2383]

General Information

Symbol

Dmel\Sgt1^s2383

Species

D. melanogaster

Name

FlyBase ID

FBal0011152

Feature type

allele

Associated gene

Dmel\Sgt1

Associated Insertion(s)

P{lacW}s2383

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{lacW}s2383

Cytology

Description

The lesion in Sgt1^s2383 mutants results in a five amino acid deletion in the Chord and Sgt1 (CS) domain that mediates Sgt1-Hsp83 protein interactions.

(Andersen et al., 2012)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:8,827,484..8,827,484 [-]

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

centrosome

(Andersen et al., 2012)

neuroblast

(Andersen et al., 2012)

neuroblast (with Df(3R)Exel6147)

(Andersen et al., 2012)

neuroblast (with Sgt1^c01428)

(Andersen et al., 2012)

spindle

(Andersen et al., 2012)

Detailed Description

Statement

Reference

Sgt1^s2383 mutants exhibit a modest decrease in neuroblast number, and larval/pupal lethality.

Sgt1^s2383/Df(3R)Exel6147 mutants exhibit a modest decrease in neuroblast number, and larval/pupal lethality.

Sgt1^s2383/Sgt1^c01428 mutants exhibit a modest decrease in neuroblast number, and larval/pupal lethality.

Sgt1^s2383 mutants exhibit slower cell cycle progression, cytokinesis failure, polyploidy, multiple centrosomes, and malformed mitotic spindles. Sgt1^s2383 mutants also exhibit defects in neuroblast cell polarity, with Sgt1 being required for establishing apical cortical polarity during prophase. Sgt1^s2383 mutants show a strong loss of cortical scrib localization during metaphase and interphase; they also show a loss of the uniform cortical pool of dlg1 protein at interphase and mitosis, with retention of the pol of dlg1 asymmetrically colocalised with raps at the apical cortex at metaphase.

Sgt1^s2383 mutant neuroblasts treated with colcemid lack all detectable apical cortical polarity.

Time-lapse imaging of Sgt1^s2383 mutant larval neuroblasts show highly abnormal cortical protrusions, misshapen cells, and failure of cytokinesis.

(Andersen et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Sgt1^s2383 has abnormal cell polarity phenotype, enhanceable by pins^P62

(Andersen et al., 2012)

NOT suppressed by

Statement

Reference

Sgt1^s2383 has abnormal cell polarity phenotype, non-suppressible by sqh^E21.Tag:FLAG

(Andersen et al., 2012)

Sgt1^s2383 has abnormal cell polarity phenotype, non-suppressible by sqh^{A20.A21.Tag:FLAG}

(Andersen et al., 2012)

Enhancer of

Statement

Reference

Sgt1^s2383 is an enhancer of abnormal cell polarity phenotype of pins^P62

(Andersen et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Sgt1^s2383 has neuroblast phenotype, suppressible by pins^P62

(Andersen et al., 2012)

NOT suppressed by

Statement

Reference

Sgt1^s2383 has neuroblast phenotype, non-suppressible by sqh^E21.Tag:FLAG

(Andersen et al., 2012)

Sgt1^s2383 has neuroblast phenotype, non-suppressible by sqh^{A20.A21.Tag:FLAG}

(Andersen et al., 2012)

Suppressor of

Statement

Reference

Sgt1^s2383 is a suppressor of neuroblast phenotype of pins^P62

(Andersen et al., 2012)

Other

Statement

Reference

Sgt1^s2383, pins^P62 has neuroblast | increased number phenotype

(Andersen et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Sgt1^s2383 raps^P62 double mutant neuroblasts establish little or no apical or basal cortical polarity. The developmental consequences of this loss of polarity are the formation of ectopic neuroblasts throughout the brain. This is in striking contrast to either single mutant, where the brains are smaller with reduced neuroblast numbers.

The presence of sqh^{E21.T:Zzzz\FLAG} fails to suppress the Sgt1^s2383 neuroblast cortical polarity phenotype.

The presence of sqh^{A20.A21.T:Zzzz\FLAG} fails to suppress the Sgt1^s2383 neuroblast cortical polarity phenotype.

(Andersen et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Sgt1^c01428

(Andersen et al., 2012)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

12075

w¹¹¹⁸; P{lacW}s2383 Sgt1^s2383/TM3, Sb¹

Notes on Origin

Discoverer

M. Scott.

The lethality and defects in neuroblast number which are seen in the l(3)s2383 insertion line do not map to the P{lacW}s2383 insertion (which is at 66E), but instead map to a separate lesion (represented by Sgt1^s2383) that maps to 84F6-84F13.

(Andersen et al., 2012)

Comments

This allele was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was discarded from the gene disruption project prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Spradling (spradling@mail1.ciwemb.edu).

Complements: l(3)01629⁰¹⁶²⁹. Complements: msn⁰³³⁴⁹. Complements: l(3)06264⁰⁶²⁶⁴. Complements: dally⁰⁶⁴⁶⁴. Complements: mRpL12¹⁰⁵³⁴. Complements: mRpL12^j4B2.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Sgt1^s2383

l(3)s2383^s2383

sgt1^s2383

(Andersen et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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