FlyBase Allele Report: Dmel\flw[1]

General Information

Symbol

Dmel\flw¹

Species

D. melanogaster

Name

FlyBase ID

FBal0004118

Feature type

allele

Associated gene

Dmel\flw

Associated Insertion(s)

Carried in Construct

Also Known As

PP1β9C¹

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Craymer and Roy, 1980)

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: V284A. Nucleotide substitution: GTG?GCG

(Raghavan et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:10,407,272..10,407,272 [+]

Nucleotide change:

T10407272C

Reported nucleotide change:

T?C

Amino acid change:

V415A | flw-PA; V284A | flw-PB; V284A | flw-PC; V284A | flw-PD

Reported amino acid change:

V284A

(Raghavan et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | somatic clone

(Wang and Riechmann, 2007)

flightless

(Raghavan et al., 2000)

viable

(Vereshchagina et al., 2004)

visible | dominant

(Craymer and Roy, 1980)

Phenotype Manifest In

Detailed Description

Statement

Reference

Hemizygous males have wild-type wings.

(Kirchner et al., 2007)

flw¹ mutant follicle cell clones generate an irregular wavy egg chamber epithelium. Mutant egg chambers are not round or ellipsoid like in wild-type but are either stretched or develop bulges.

(Wang and Riechmann, 2007)

flw¹ hemizygotes have normal wings.

(Bennett et al., 2006)

flw¹/flw⁶ flies are completely flightless. flw¹ flies show a variable indirect flight muscle (IFM) phenotype ranging from normal through disorganised to absent IFMs. IFMs are never present in flw¹/Df(1)N110, flw¹/Df(1)Hk or flw¹/flw⁶ flies, but the jump muscle (tergal depressor of trochanter muscle) appears normal. The early development of IFMs in flw¹/Df(1)Hk pupae appears normal the pretemplates on which the dorsal longitudinal muscles form are present, the dorsoventral muscles also develop normally. Muscle defects become apparent at about 28 hours after puparium formation. Most of the muscles break away from their posterior attachment sites and are found as ball-like structures at the anterior segment boundaries, the sarcomere organisation in these balls appears normal.

(Raghavan et al., 2000)

DLM and DVMs are scant. Myofibrils are disorganized, with variable fiber diameter, and Z bands are crooked.

(Deak et al., 1982)

Hemizygotes have bubbles beneath the thoracic cuticle and held up wings.

(Craymer and Roy, 1980)

RK2.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

flw¹ has viable phenotype, non-enhanceable by Mbs³

(Vereshchagina et al., 2004)

flw¹ has viable phenotype, non-enhanceable by sqh^A20.Tag:FLAG

(Vereshchagina et al., 2004)

Suppressed by

Statement

Reference

flw¹, puc^A251.1F3/puc[+] has visible phenotype, suppressible by bsk¹/bsk[+]

(Kirchner et al., 2007)

flw¹, puc^A251.1F3/puc[+] has visible phenotype, suppressible by zip[+]/zip¹

(Kirchner et al., 2007)

flw¹, puc^A251.1F3/puc[+] has visible phenotype, suppressible by sqh^E20.E21

(Kirchner et al., 2007)

Pp1α-96A^GS11179/Df(3R)crb87-5, flw¹ has lethal phenotype, suppressible by zip¹

(Kirchner et al., 2007)

NOT suppressed by

Statement

Reference

flw¹, puc^A251.1F3/puc[+] has visible phenotype, non-suppressible by sqh^{A20.A21.Tag:FLAG}

(Kirchner et al., 2007)

Suppressor of

Statement

Reference

flw¹ is a suppressor of viable phenotype of Nop17l^{UAS.Tag:polyHis,Tag:MYC}, Scer\GAL4^arm.PS

(Bennett et al., 2006)

NOT Suppressor of

Statement

Reference

flw^UAS.Tag:HA/flw¹, Scer\GAL4^arm.PS is a non-suppressor of viable phenotype of Nop17l^{UAS.Tag:polyHis,Tag:MYC}, Scer\GAL4^arm.PS

(Bennett et al., 2006)

Other

Statement

Reference

flw¹, puc^67BDn/puc[+] has partially lethal - majority die phenotype

(Kirchner et al., 2007)

flw¹, puc^67BDn/puc[+] has visible phenotype

(Kirchner et al., 2007)

flw¹, puc^A251.1F3/puc[+] has viable phenotype

(Kirchner et al., 2007)

flw¹, puc^A251.1F3/puc[+] has visible phenotype

(Kirchner et al., 2007)

Mbs³/Mbs[+], flw¹, puc^A251.1F3/puc[+] has lethal phenotype

(Kirchner et al., 2007)

Mbs³/Mbs[+], flw¹ has viable phenotype

(Kirchner et al., 2007)

Df(3L)AC1, flw¹ has lethal phenotype

(Kirchner et al., 2007)

Pp1α-96A^GS11179/Pp1α-96A[+], flw¹ has viable phenotype

(Kirchner et al., 2007)

Df(3R)crb87-5/+, flw¹ has viable phenotype

(Kirchner et al., 2007)

Pp1α-96A^GS11179/Df(3R)crb87-5, flw¹ has lethal phenotype

(Kirchner et al., 2007)

Pp1-87B^87Bg-3/Pp1-87B[+], flw¹ has viable phenotype

(Kirchner et al., 2007)

MYPT-75D^{HM.UAS.Tag:MYC,}, Scer\GAL4^arm.PS, flw¹ has partially lethal - majority die | male phenotype

(Vereshchagina et al., 2004)

MYPT-75D^{HM.UAS.Tag:MYC,}, Scer\GAL4^hs.PB, flw¹ has partially lethal - majority die | male phenotype

(Vereshchagina et al., 2004)

flw¹, sqh^E20.E21 has lethal | male phenotype

(Vereshchagina et al., 2004)

flw¹, smi26D¹ has abnormal smell perception | dominant | female phenotype

(Anholt et al., 2003)

flw¹, smi28E¹ has abnormal smell perception | dominant | female phenotype

(Anholt et al., 2003)

flw¹, smi26D¹/smi26D[+] has abnormal smell perception | dominant | female phenotype

(Anholt et al., 2003)

flw¹, smi28E[+]/smi28E¹ has abnormal smell perception | dominant | female phenotype

(Anholt et al., 2003)

Phenotype Manifest In

Suppressed by

Statement

Reference

flw¹, puc^A251.1F3/puc[+] has wing phenotype, suppressible by bsk¹/bsk[+]

(Kirchner et al., 2007)

flw¹, puc^A251.1F3/puc[+] has wing phenotype, suppressible by zip[+]/zip¹

(Kirchner et al., 2007)

flw¹, puc^A251.1F3/puc[+] has wing phenotype, suppressible by sqh^E20.E21

(Kirchner et al., 2007)

NOT suppressed by

Statement

Reference

flw¹, puc^A251.1F3/puc[+] has wing phenotype, non-suppressible by sqh^{A20.A21.Tag:FLAG}

(Kirchner et al., 2007)

flw¹ has phenotype, non-suppressible by Mlc2^S66A,S67A

(Raghavan et al., 2000)

Enhancer of

Statement

Reference

flw¹ is an enhancer of wing phenotype of Scer\GAL4^vg.PU, hep^CA.UAS

(Kirchner et al., 2007)

flw¹ is an enhancer of wing phenotype of Nop17l^{UAS.Tag:polyHis,Tag:MYC}, Scer\GAL4^Bx-MS1096

(Bennett et al., 2006)

flw¹ is an enhancer of posterior wing margin phenotype of Nop17l^{UAS.Tag:polyHis,Tag:MYC}, Scer\GAL4^Bx-MS1096

(Bennett et al., 2006)

NOT Enhancer of

Statement

Reference

flw[+]/flw¹ is a non-enhancer of egg | germline clone phenotype of Pkn^1T

(Ferreira et al., 2014)

flw[+]/flw¹ is a non-enhancer of egg | germline clone phenotype of Pkn^2T

(Ferreira et al., 2014)

Other

Statement

Reference

flw¹, puc^67BDn/puc[+] has metathoracic leg phenotype

(Kirchner et al., 2007)

flw¹, puc^A251.1F3/puc[+] has wing phenotype

(Kirchner et al., 2007)

flw¹, puc^A251.1F3/puc[+] has metathoracic leg phenotype

(Kirchner et al., 2007)

flw¹, puc^67BDn/puc[+] has wing phenotype

(Kirchner et al., 2007)

flw¹, sqh^E21.Tag:FLAG has wing phenotype

(Vereshchagina et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

flw¹/+ does not enhance the shorter egg length phenotype seen in Pkn^1T/Pkn^1T or Pkn^2T/Pkn^2T germline clones.

(Ferreira et al., 2014)

The flw¹/Y ; puc^67BDn/+ combination is semi-lethal (90% of pupae fail to eclose) and surviving males have strong wing defects and occasional third leg malformations.

The flw¹/Y ; puc^A251.1F3/+ combination is viable. 30% of these flies have wing phenotypes that range from slight defects in the posterior part of the wing to stumps. Flies with the most severe wing phenotypes sometimes have third leg malformations.

The wing defects caused by expression of hep^CA.Scer\UAS under the control of Scer\GAL4^vg.PU are enhanced in a flw¹/Y background; the wings are much smaller and are frequently notched and blistered. The double mutant flies are only 10% viable relative to their flw¹/+ ; hep^CA.Scer\UAS, Scer\GAL4^vg.PU control siblings.

The wing defects seen in flw¹/Y ; puc^A251.1F3/+ flies are suppressed to wild type by bsk¹/+, zip¹/+ or sqh^E20.E21.

The wing defects seen in flw¹/Y ; puc^A251.1F3/+ flies are not suppressed by sqh^{A20.A21.T:Zzzz\FLAG}.

(Kirchner et al., 2007)

Expression of Nop17l^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096 in hemizygous flw¹ mutant wings results in severely crumpled and reduced wings.

Expression of Nop17l^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} under the control of Scer\GAL4^arm.PS is synthetic lethal in a hemizygous flw¹ mutant background.

The synthetic lethality resulting from the overexpression of Nop17l^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} under the control of Scer\GAL4^arm.PS in a hemizygous flw¹ mutant background can be rescued by the co-expression of flw^{Scer\UAS.T:Ivir\HA1}.

(Bennett et al., 2006)

The flw¹ mutation is lethal in combination with the phosphomimetic sqh^E20.E21 mutation. Most flw¹/Y; sqh^{E21.T:Zzzz\FLAG}/+ flies are viable, but have a crumpled wing phenotype, resembling that of stronger flw alleles. The Mbs³ mutation does not affect the viability of flw¹ mutants.

(Vereshchagina et al., 2004)

The addition of Mlc2^S66A,S67A does not suppress flw mutant phenotypes.

(Raghavan et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

flw¹

186

y² VhaAC39-1^cho-2 flw¹

Kyoto

105778

y² VhaAC39-1^cho-2 flw¹

Notes on Origin

Discoverer

Waletzky, 1937.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

PP1β9C¹

(Kirchner et al., 2007, Bennett et al., 2006)

flp

flw¹

(Zeledon et al., 2019, Ferreira et al., 2014, Popodi et al., 2010-, Kirchner et al., 2007, Wang and Riechmann, 2007, Bennett et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

Open Close