FB2024_03 , released June 25, 2024
Allele: Dmel\Mlc2S66A,S67A
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General Information
Symbol
Dmel\Mlc2S66A,S67A
Species
D. melanogaster
Name
FlyBase ID
FBal0044946
Feature type
allele
Associated gene
Dmel\Mlc2
Associated Insertion(s)
Carried in Construct
P{Mlc2S66A,S67A}
Key Links
Transgenic product class
missense_variant
(Tohtong et al., 1995)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Tohtong et al., 1995)
Mutagen
in vitro construct
(Sparrow, 1995, Tohtong et al., 1995)
Progenitor genotype
Mlc2+tTa
(Sparrow, 1995, Tohtong et al., 1995)
Carried in construct
P{Mlc2S66A,S67A}
Cytology
Description

Construct: Amino acid replacement: S66A. Amino acid replacement: S67A.

(Sparrow, 1995)

Amino acid replacement: S66A. Amino acid replacement: S67A.

(Tohtong et al., 1995)
Allele components
Component
Use(s)
Regulatory region(s)
Mlc2
Encoded product / tool
Mlc2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Mlc2E38 mutant flies expressing Mlc2S66A,S67A display decreased wing beat frequency and few of them are able to fly or beat their wings resulting in a large reduction in flight index.

      (Miller et al., 2011)

      Mlc2E38 mutants carrying Mlc2S66A,S67A display sarcomeric structural abnormalities as revealed by X-ray diffraction and electron microscopy.

      (Farman et al., 2009)

      Most flies carrying Mlc2S66A,S67A are unable to beat their wings.

      (Irving and Maughan, 2000)

      The myofibrillar and sarcomeric structures of the indirect flight muscle (IFM) of Mlc2E38 flies carrying Mlc2S66A,S67A appear normal. IFM fibers fixed in the rigor state have well-ordered cross-bridge arrays. The flight index of these flies is significantly less than for Mlc2E38 flies carrying Mlc2+tTa (0.9 +/- 0.6). The wingbeat frequency of these flies is 152 s-1.

      (Dickinson et al., 1997)

      Indirect flight muscles appear normal.

      (Sparrow, 1995)

      Indirect flight muscles appear normal. Two copies of P{Mlc2S66A,S67A} restores viability to Mlc2E38 homozygotes.

      (Tohtong et al., 1995)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Suppressor of
      Statement
      Reference

      Mlc2S66A,S67A is a non-suppressor of phenotype of flwG0172

      (Vereshchagina et al., 2004)

      Mlc2S66A,S67A is a non-suppressor of phenotype of flw1

      (Raghavan et al., 2000)

      Mlc2S66A,S67A is a non-suppressor of phenotype of flw6

      (Raghavan et al., 2000)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Mlc2S66A,S67A partially rescues Mlc2E38

      (Tohtong et al., 1995)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Mlc2 protein cannot be phosphorylated at serine-66 or serine-67.

      (Sparrow, 1995, Tohtong et al., 1995)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Mlc2S66,67A
      (Irving and Maughan, 2000)
      Mlc2S66A,S67A
      Name Synonyms
      Secondary FlyBase IDs
        References (9)