Amino acid replacement: ?177term.
C9265888T
Q162term | Pkn-PB; Q162term | Pkn-PC; Q177term | Pkn-PF; Q177term | Pkn-PD; Q177term | Pkn-PJ; Q256term | Pkn-PK; Q256term | Pkn-PG; Q256term | Pkn-PH; Q162term | Pkn-PM; Q177term | Pkn-PL; Q162term | Pkn-PN; Q162term | Pkn-PO; Q162term | Pkn-PP; Q162term | Pkn-PQ; Q256term | Pkn-PS
Q177term
Site of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change. The isoforms relative to which the mutation was mapped was not specified.
Females with Pkn2T/Pkn2T germline clones show no significant difference in total number of eggs or egg laying distribution compared to controls, but egg length is significantly reduced (though dorsal appendages are usually normally positioned).
During nurse cell dumping, egg chambers mutant for Pkn2T/Pkn2T show increased nurse cell-to-oocyte membrane collapse, leading to abnormal presence of ring canals, border cells and/or nurse cell nuclei in the oocyte cytoplasm.
Nurse cell adherens junctions and somatic follicular epithelial cells look normal, and no significant morphological defects are detected in ring canals or nurse cell cytoplasmic actin cables in Pkn2T/Pkn2T mutant germline clone egg chambers during early oogenesis. In mid-oogenesis there is an increase in the frequency of nurse cell-to-nurse cell membrane collapse (leading to multinucleated nurse cells after stage 6/7) in Pkn2T/Pkn2T mutant germline clone egg chambers.
Females with Pkn2T/Pkn2T germline clones show high abnormal accumulation of F-actin and myosin in nurse cells of stage 10 egg chambers, with an abnormally thick activated actin-myosin cytoskeleton with significant discontinuities that frequently radiates outwardly perpendicular to the anterior-posterior axis of the egg chamber.
Average nurse cell slow dumping rates in egg chambers from freshly dissected ovaries of Pkn2T/Pkn2T germline clones are not significantly different to controls, whereas fast dumping rates are significantly slower than controls.
Pkn2T has egg | germline clone phenotype, enhanceable by sqhRLC.GFP(S65T)/sqhRLC.GFP(S65T)
Pkn2T has egg | germline clone phenotype, enhanceable by sqhE20.E21/sqh[+]
Pkn2T has egg | germline clone phenotype, enhanceable by flw[+]/flwG0172
Pkn2T has egg | germline clone phenotype, non-enhanceable by Scer\GAL4nanos.PU/RokUAS.Tag:HA
Pkn2T has egg | germline clone phenotype, non-enhanceable by flw[+]/flw1
Pkn2T has egg | germline clone phenotype, suppressible | partially by cpbM143/cpb[+]
Pkn2T has nurse cell | germline clone phenotype, suppressible | partially by cpbM143/cpb[+]
Pkn2T/Pkn2T is an enhancer of egg phenotype of sqhE20.E21
Pkn2T/Pkn2T is a non-enhancer | germline clone of egg phenotype of RokUAS.Tag:HA, Scer\GAL4nanos.PU
Presence of cpbM143/+ partially suppresses the shorter egg length and nurse cell F-actin accumulation seen in Pkn2T/Pkn2T germline clone egg chambers.
sqhRLC.T:Avic\GFP-S65T/sqhRLC.T:Avic\GFP-S65T or sqhE20.E21/+ or flwG0172/+ enhances the shorter egg length phenotype seen in Pkn2T/Pkn2T germline clones.
Overexpression of RokScer\UAS.T:Ivir\HA1 driven by Scer\GAL4nos.PU, or flw1/+, does not enhance the shorter egg length phenotype seen in Pkn2T/Pkn2T germline clones.
The shorter egg length phenotype seen in females with overexpression of RokScer\UAS.T:Ivir\HA1 driven by Scer\GAL4nos.PU is not enhanced in Pkn2T/Pkn2T germline clones.