FlyBase Allele Report: Ggal\MLCK[ct.UAS]

General Information

Symbol

Ggal\MLCK^ct.UAS

Species

G. gallus

Name

FlyBase ID

FBal0141735

Feature type

allele

Associated gene

Ggal\MLCK

Associated Insertion(s)

Carried in Construct

P{UAS-MLCK.ct}

Also Known As

UAS-ctMLCK, UAS-MLCK.ct, UAS-MLCK

Transgenic product class

stop_gained

(Kim et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

stop_gained

(Kim et al., 2002)

Mutagen

in vitro construct

(Fritz and VanBerkum, 2002, Kim et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-MLCK.ct}

Cytology

Description

UASt regulatory sequences drive expression of constitutively active form of Ggal\MLCK (a truncated form in which the autoinhibitory domain has been deleted by introducing two adjacent stop codons after residue 779).

(Kim et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Ggal\MLCK

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | adult stage, with Scer\GAL4^GMR.PU

(Rosa-Birriel et al., 2024)

abnormal cell shape | embryonic stage, with Scer\GAL4^da.PU

(West et al., 2017)

abnormal neuroanatomy, with Scer\GAL4^elav.PLu

(Kim et al., 2002)

abnormal neuroanatomy, with Scer\GAL4^ftz.ng

(Kim et al., 2002)

abnormal neuroanatomy | embryonic stage 16, with Scer\GAL4^ftz.ng

(Dorsten et al., 2007)

lethal, with Scer\GAL4^elav.PLu

(Kim et al., 2002)

Phenotype Manifest In

adherens junction | adult stage, with Scer\GAL4^GMR.PU

(Rosa-Birriel et al., 2024)

amnioserosa, with Scer\GAL4^c381

(Homem and Peifer, 2008)

amnioserosa, with Scer\GAL4^prd.RG1

(Homem and Peifer, 2008)

commissure | embryonic stage 16, with Scer\GAL4^ftz.ng

(Dorsten et al., 2007)

dMP2 neuron, with Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

embryonic epidermis, with Scer\GAL4^da.PU

(West et al., 2017)

embryonic epidermis, with Scer\GAL4^prd.RG1

(Homem and Peifer, 2008)

epithelial cell | embryonic stage, with Scer\GAL4^da.PU

(West et al., 2017)

eye, with Scer\GAL4^GMR.PU

(Rosa-Birriel et al., 2024)

larval longitudinal connective, with Scer\GAL4^elav.PLu

(Kim et al., 2002)

larval longitudinal connective, with Scer\GAL4^ftz.ng

(Kim et al., 2002)

larval ventral nerve cord commissure | embryonic stage 16, with Scer\GAL4^ftz.ng

(Dorsten et al., 2007)

pCC neuron, with Scer\GAL4^elav.PLu

(Kim et al., 2002)

pCC neuron, with Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002, Kim et al., 2002)

pigment cell | adult stage, with Scer\GAL4^GMR.PU

(Rosa-Birriel et al., 2024)

vMP2 neuron, with Scer\GAL4^elav.PLu

(Kim et al., 2002)

vMP2 neuron, with Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

Detailed Description

Statement

Reference

The expression of Ggal\MLCK^ct.Scer\UAS under the control of Scer\GAL4^da.PU results in hyper-constriction of epidermal leading edge cells during dorsal closure, as compared to controls.

(West et al., 2017)

Expression of Ggal\MLCK^ct.Scer\UAS under the control of Scer\GAL4^c381 results in uniform amnioserosal cell rounding.

Expression of Ggal\MLCK^ct.Scer\UAS under the control of Scer\GAL4^prd.RG1 in individual amnioserosa cells induces premature apical constriction in these cells.

Expression of Ggal\MLCK^ct.Scer\UAS under the control of Scer\GAL4^prd.RG1 results in deep segmental grooves in the embryo.

(Homem and Peifer, 2008)

Stage 16 embryos overexpressing Ggal\MLCK^ct.Scer\UAS driven by Scer\GAL4^ftz.ng display axon bundles that cross the midline inappropriately.

(Dorsten et al., 2007)

When Ggal\MLCK^ct.Scer\UAS is driven by Scer\GAL4^ftz.ng, midline crossovers are seen in the pCC/MP2 pathway axons in 8.2% of embryos. An average of 1.4 crossovers are seen per embryo.

(Fritz and VanBerkum, 2002)

Expression of Ggal\MLCK^ct.Scer\UAS under the control of Scer\GAL4^elav.PLu causes specific defects in the formation of the pCC/MP2 pathway. Initial extension of the pCC axons at stage 12 appears normal. By stage 14, the common MP2 pathway and the separate MP1 pathway are formed normally, but some of the axons of the vMP2 pathway cross the midline. By stage 16, pCC/MP2 pathway axons are seen to cross the midline in several segments, while the remaining longitudinal connectives appear unaffected. Expression of Ggal\MLCK^ct.Scer\UAS under the control of Scer\GAL4^ftz.ng causes axons of the pCC/MP2 pathway to cross the midline incorrectly in 9-19% of embryos.

(Kim et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng, fra^UAS.cKa has abnormal neuroanatomy | embryonic stage 16 phenotype, enhanceable by Abl⁴

(Dorsten et al., 2007)

Abl⁴/Abl[+], Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has abnormal neuroanatomy | embryonic stage 16 phenotype, enhanceable by Rho1^V14.UAS, Scer\GAL4^ftz.ng

(Dorsten et al., 2007)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has abnormal neuroanatomy | embryonic stage 16 phenotype, enhanceable by fra^UAS.cKa, Scer\GAL4^ftz.ng

(Dorsten et al., 2007)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has abnormal neuroanatomy phenotype, enhanceable by robo[+]/robo1¹

(Kim et al., 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has abnormal neuroanatomy phenotype, enhanceable by sli²/sli[+]

(Kim et al., 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has abnormal neuroanatomy phenotype, enhanceable by sqh^E20.E21

(Kim et al., 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has abnormal neuroanatomy phenotype, enhanceable by fra^UAS.cKa, Scer\GAL4^ftz.ng

(Kim et al., 2002)

Suppressed by

Statement

Reference

Ggal\MLCK^ct.UAS, Rho1^V14.UAS, Scer\GAL4^ftz.ng has abnormal neuroanatomy | embryonic stage 16 phenotype, suppressible by sqh^{T20A.S21A.UAS}, Scer\GAL4^ftz.ng

(Dorsten et al., 2007)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has abnormal neuroanatomy | embryonic stage 16 phenotype, suppressible by Abl⁴/Abl[+]

(Dorsten et al., 2007)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng, fra^UAS.cKa has abnormal neuroanatomy | embryonic stage 16 phenotype, suppressible by Abl⁴/Abl[+]

(Dorsten et al., 2007)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has abnormal neuroanatomy phenotype, suppressible by fra³/fra[+]

(Kim et al., 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has abnormal neuroanatomy phenotype, suppressible | partially by fra⁴/fra[+]

(Kim et al., 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has abnormal neuroanatomy phenotype, suppressible | partially by sqh^{A20.A21.Tag:FLAG}

(Kim et al., 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has abnormal neuroanatomy phenotype, suppressible | partially by sqh¹

(Kim et al., 2002)

Enhancer of

Statement

Reference

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng is an enhancer of abnormal neuroanatomy | embryonic stage 16 phenotype of Rac1^V12.UAS, Scer\GAL4^ftz.ng

(Dorsten et al., 2007)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng is an enhancer of abnormal neuroanatomy | embryonic stage 16 phenotype of Rho1^V14.UAS, Scer\GAL4^ftz.ng, fra^UAS.cKa

(Dorsten et al., 2007)

Ggal\MLCK^ct.UAS/Scer\GAL4^ftz.ng is an enhancer of abnormal neuroanatomy phenotype of robo1¹

(Kim et al., 2002)

Ggal\MLCK^ct.UAS/Scer\GAL4^ftz.ng is an enhancer of abnormal neuroanatomy phenotype of sli²

(Kim et al., 2002)

Suppressor of

Statement

Reference

Ggal\MLCK^ct.UAS/Scer\GAL4^elav.PLu is a suppressor of abnormal neuroanatomy phenotype of comm¹

(Kim et al., 2002)

Ggal\MLCK^ct.UAS/Scer\GAL4^ftz.ng is a suppressor of abnormal neuroanatomy phenotype of comm¹

(Kim et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng, fra^UAS.cKa has commissure | embryonic stage 16 phenotype, enhanceable by Abl⁴

(Dorsten et al., 2007)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng, fra^UAS.cKa has larval ventral nerve cord commissure | embryonic stage 16 phenotype, enhanceable by Abl⁴

(Dorsten et al., 2007)

Abl⁴/Abl[+], Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has commissure | embryonic stage 16 phenotype, enhanceable by Rho1^V14.UAS, Scer\GAL4^ftz.ng

(Dorsten et al., 2007)

Abl⁴/Abl[+], Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has larval ventral nerve cord commissure | embryonic stage 16 phenotype, enhanceable by Rho1^V14.UAS, Scer\GAL4^ftz.ng

(Dorsten et al., 2007)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has commissure | embryonic stage 16 phenotype, enhanceable by fra^UAS.cKa, Scer\GAL4^ftz.ng

(Dorsten et al., 2007)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has larval ventral nerve cord commissure | embryonic stage 16 phenotype, enhanceable by fra^UAS.cKa, Scer\GAL4^ftz.ng

(Dorsten et al., 2007)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has dMP2 neuron phenotype, enhanceable by Rac1^V12.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has pCC neuron phenotype, enhanceable by Rac1^V12.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has vMP2 neuron phenotype, enhanceable by Rac1^V12.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has dMP2 neuron phenotype, enhanceable by Rho1^N19.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has vMP2 neuron phenotype, enhanceable by Rho1^N19.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has pCC neuron phenotype, enhanceable by Rho1^N19.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has dMP2 neuron phenotype, enhanceable by Rho1^V14.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has pCC neuron phenotype, enhanceable by Rho1^V14.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has vMP2 neuron phenotype, enhanceable by Rho1^V14.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has dMP2 neuron phenotype, enhanceable by Cdc42^V12.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has pCC neuron phenotype, enhanceable by Cdc42^V12.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has vMP2 neuron phenotype, enhanceable by Cdc42^V12.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has larval longitudinal connective phenotype, enhanceable by fra^UAS.cKa, Scer\GAL4^ftz.ng

(Kim et al., 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has larval longitudinal connective phenotype, enhanceable by robo[+]/robo1¹

(Kim et al., 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has larval longitudinal connective phenotype, enhanceable by sli²/sli[+]

(Kim et al., 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has larval longitudinal connective phenotype, enhanceable by sqh^E20.E21

(Kim et al., 2002)

Suppressed by

Statement

Reference

Ggal\MLCK^ct.UAS, Rho1^V14.UAS, Scer\GAL4^ftz.ng has commissure | embryonic stage 16 phenotype, suppressible by sqh^{T20A.S21A.UAS}, Scer\GAL4^ftz.ng

(Dorsten et al., 2007)

Ggal\MLCK^ct.UAS, Rho1^V14.UAS, Scer\GAL4^ftz.ng has larval ventral nerve cord commissure | embryonic stage 16 phenotype, suppressible by sqh^{T20A.S21A.UAS}, Scer\GAL4^ftz.ng

(Dorsten et al., 2007)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has commissure | embryonic stage 16 phenotype, suppressible by Abl⁴/Abl[+]

(Dorsten et al., 2007)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has larval ventral nerve cord commissure | embryonic stage 16 phenotype, suppressible by Abl⁴/Abl[+]

(Dorsten et al., 2007)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng, fra^UAS.cKa has commissure | embryonic stage 16 phenotype, suppressible by Abl⁴/Abl[+]

(Dorsten et al., 2007)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng, fra^UAS.cKa has larval ventral nerve cord commissure | embryonic stage 16 phenotype, suppressible by Abl⁴/Abl[+]

(Dorsten et al., 2007)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has pCC neuron phenotype, suppressible | partially by Cdc42^N17.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has pCC neuron phenotype, suppressible | partially by Rac1^N17.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has vMP2 neuron phenotype, suppressible | partially by Cdc42^N17.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has vMP2 neuron phenotype, suppressible | partially by Rac1^N17.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has dMP2 neuron phenotype, suppressible | partially by Cdc42^N17.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has dMP2 neuron phenotype, suppressible | partially by Rac1^N17.UAS, Scer\GAL4^ftz.ng

(Fritz and VanBerkum, 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has larval longitudinal connective phenotype, suppressible by fra³/fra[+]

(Kim et al., 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has larval longitudinal connective phenotype, suppressible | partially by fra⁴/fra[+]

(Kim et al., 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has larval longitudinal connective phenotype, suppressible | partially by sqh¹

(Kim et al., 2002)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng has larval longitudinal connective phenotype, suppressible | partially by sqh^{A20.A21.Tag:FLAG}

(Kim et al., 2002)

Enhancer of

Statement

Reference

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng is an enhancer of commissure | embryonic stage 16 phenotype of Rac1^V12.UAS, Scer\GAL4^ftz.ng

(Dorsten et al., 2007)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng is an enhancer of larval ventral nerve cord commissure | embryonic stage 16 phenotype of Rac1^V12.UAS, Scer\GAL4^ftz.ng

(Dorsten et al., 2007)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng is an enhancer of commissure | embryonic stage 16 phenotype of Rho1^V14.UAS, Scer\GAL4^ftz.ng, fra^UAS.cKa

(Dorsten et al., 2007)

Ggal\MLCK^ct.UAS, Scer\GAL4^ftz.ng is an enhancer of larval ventral nerve cord commissure | embryonic stage 16 phenotype of Rho1^V14.UAS, Scer\GAL4^ftz.ng, fra^UAS.cKa

(Dorsten et al., 2007)

Ggal\MLCK^ct.UAS/Scer\GAL4^ftz.ng is an enhancer of larval longitudinal connective phenotype of robo1¹

(Kim et al., 2002)

Ggal\MLCK^ct.UAS/Scer\GAL4^ftz.ng is an enhancer of larval longitudinal connective phenotype of sli²

(Kim et al., 2002)

Suppressor of

Statement

Reference

Ggal\MLCK^ct.UAS/Scer\GAL4^e22c is a suppressor of amnioserosa | maternal effect phenotype of Rho1^72O/Rho1[+], dia²

(Homem and Peifer, 2008)

Ggal\MLCK^ct.UAS/Scer\GAL4^elav.PLu is a suppressor of symmetrical commissure phenotype of comm¹

(Kim et al., 2002)

Ggal\MLCK^ct.UAS/Scer\GAL4^ftz.ng is a suppressor of symmetrical commissure phenotype of comm¹

(Kim et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The abnormal cell protrusions that are seen extending from amnioserosa cells in the progeny of dia² Rho1^72O double heterozygous females during dorsal closure are suppressed by expression of Ggal\MLCK^ct.Scer\UAS under the control of Scer\GAL4^e22c.

(Homem and Peifer, 2008)

Co-expression of Ggal\MLCK^ct.Scer\UAS with Rac1^V12.Scer\UAS under the control of Scer\GAL4^ftz.ng results in a synergistic increase in the frequency of incorrect axon projections across the midline, compared with Rac1^V12.Scer\UAS-overexpression alone.

When fra^Scer\UAS.cKa is co-expressed with both Rho1^V14.Scer\UAS and Ggal\MLCK^ct.Scer\UAS under the control of Scer\GAL4^ftz.ng, all embryos exhibit severe defects in scaffold formation. Many ventral nerve cord axons either cross the midline or collapse into the midline region.

Co-expression of sqh^{T20A.S21A.Scer\UAS} under the control of Scer\GAL4^ftz.ng suppresses the midline crossing phenotype in embryos expressing both Rho1^V14.Scer\UAS and Ggal\MLCK^ct.Scer\UAS.

No embryos heterozygous for Abl⁴ and expressing Ggal\MLCK^ct.Scer\UAS under the control of Scer\GAL4^ftz.ng exhibit axonal midline crossing defects.

Co-expression of fra^Scer\UAS.cKa with Ggal\MLCK^ct.Scer\UAS under the control of Scer\GAL4^ftz.ng increases the frequency of axonal midline crossing abnormalities.

Heterozygous Abl⁴ almost completely suppresses the axonal midline crossing phenotype of embryos co-expressing Ggal\MLCK^ct.Scer\UAS with fra^Scer\UAS.cKa under the control of Scer\GAL4^ftz.ng.

Homozygous Abl⁴ enhances the axonal midline crossing phenotype of embryos co-expressing Ggal\MLCK^ct.Scer\UAS with fra^Scer\UAS.cKa under the control of Scer\GAL4^ftz.ng.

The heterozygous Abl⁴-dependent suppression of the axonal midline crossing phenotype in embryos expressing Ggal\MLCK^ct.Scer\UAS under the control of Scer\GAL4^ftz.ng is lost if Rho1^V14.Scer\UAS is also expressed.

(Dorsten et al., 2007)

The addition of Rho1^N19.Scer\UAS to Ggal\MLCK^ct.Scer\UAS, Scer\GAL4^ftz.ng embryos enhances the midline crossover phenotype seen in the pCC/MP2 pathway axons. 28% of embryos exhibit the phenotype. An average of 1.3 crossovers are seen per embryo. The addition of Rho1^V14.Scer\UAS to Ggal\MLCK^ct.Scer\UAS, Scer\GAL4^ftz.ng embryos enhances the midline crossover phenotype seen in the pCC/MP2 pathway axons. 45% of embryos exhibit the phenotype. An average of 2.1 crossovers are seen per embryo. The addition of Rac1^N17.Scer\UAS to Ggal\MLCK^ct.Scer\UAS, Scer\GAL4^ftz.ng embryos partially suppresses the midline crossover phenotype seen in the pCC/MP2 pathway axons. 4.0% of embryos exhibit the phenotype. An average of 1.o crossovers are seen per embryo. The addition of Rac1^V12.Scer\UAS to Ggal\MLCK^ct.Scer\UAS, Scer\GAL4^ftz.ng embryos enhances the midline crossover phenotype seen in the pCC/MP2 pathway axons. 95% of embryos exhibit the phenotype. An average of 4.2 crossovers are seen per embryo. The addition of Cdc42^N17.Scer\UAS to Ggal\MLCK^ct.Scer\UAS, Scer\GAL4^ftz.ng embryos partially suppresses the midline crossover phenotype seen in the pCC/MP2 pathway axons. 4.4% of embryos exhibit the phenotype. An average of 1.3 crossovers are seen per embryo. The addition of Cdc42^V12.Scer\UAS to Ggal\MLCK^ct.Scer\UAS, Scer\GAL4^ftz.ng embryos enhances the midline crossover phenotype seen in the pCC/MP2 pathway axons. 97% of embryos exhibit the phenotype. An average of 7.8 crossovers are seen per embryo.

(Fritz and VanBerkum, 2002)

The midline crossover phenotype caused expression of Ggal\MLCK^ct.Scer\UAS under the control of Scer\GAL4^ftz.ng is partially suppressed by sqh¹ or sqh^{A20.A21.T:Zzzz\FLAG} and is enhanced by sqh^E20.E21. Fas2-positive axon bundles cross the midline in about 83% or 88% of robo¹/+ embryos which are also expressing Ggal\MLCK^ct.Scer\UAS under the control of Scer\GAL4^ftz.ng. Fas2-positive axon bundles cross the midline in 100% of sli²/+ embryos which are also expressing Ggal\MLCK^ct.Scer\UAS under the control of Scer\GAL4^ftz.ng. The midline crossover phenotype caused expression of Ggal\MLCK^ct.Scer\UAS under the control of Scer\GAL4^ftz.ng is partially suppressed by fra⁴/+ and completely suppressed by fra³/+. The midline crossover phenotype caused expression of Ggal\MLCK^ct.Scer\UAS under the control of Scer\GAL4^ftz.ng is enhanced by coexpression of fra^Scer\UAS.cKa. Expression of Ggal\MLCK^ct.Scer\UAS under the control of Scer\GAL4^ftz.ng in comm¹ embryos results in 49% of embryos showing some axons crossing the midline and in many cases thin commissures are present. Expression of Ggal\MLCK^ct.Scer\UAS under the control of Scer\GAL4^elav.PLu in comm¹ embryos results in partial commissure formation in 71% of embryos.

(Kim et al., 2002)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

37528

w¹¹¹⁸; P{UAS-MLCK.ct}87.11/CyO, P{elav-lacZ.H}YH2

37527

w¹¹¹⁸; P{UAS-MLCK.ct}87/TM3, P{ftz-lacZ.ry⁺}TM3, Sb¹ ry^*

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ggal\MLCK^ct.Scer\UAS

Ggal\MLCK^ct.UAS

MLCK^CA

(Rosa-Birriel et al., 2024)

Name Synonyms

Secondary FlyBase IDs

References (12)

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