FlyBase Allele Report: Dmel\Moe[G0323]

(Schottenfeld-Roames et al., 2014, Neisch et al., 2010, Hipfner et al., 2004, Karagiosis and Ready, 2004, Speck et al., 2003, Jankovics et al., 2002, Peter et al., 2002, Schäfer et al., 1999.6.15)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

AJ426795

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

R2-R5 axons do not terminate in the lamina in hemizygous third instar larvae, but instead project into the medulla. This phenotype is 100% penetrant. The morphology of the R8 growth cones is abnormal.

Homozygous R8 axon MARCM clones show severe targeting defects and have unusually large growth cones with many fine processes. Homozygous R2-R5 axon MARCM clones show appropriate targeting to the lamina region.

(Ruan et al., 2013)

Imaginal discs from hemizygous Moe^G0323 mutant animals are much smaller than those of wild-type animals. The number of apoptotic cells is increased in the mutant imaginal discs compared with wild-type.

(Neisch et al., 2010)

Mutant animals develop more slowly than wild-type, reach pupal stages later and die as pupae.

(Hipfner et al., 2004)

Hemizygous males show late larval lethality. Cells in Moe^G0323 imaginal discs show depletion of F-actin from the apical region (where it normally accumulates in wild-type cells) and accumulation of F-actin at ectopic sites within the cell. Cells lacking apical-basal polarity accumulate basal to the epithelial layer in Moe^G0323 imaginal discs. Cells marked by Avic\GFP expression in the Moe^G0323 wing disc appear to disperse from their normal position and can invade adjacent regions of the disc. The epithelial ultrastructure is disrupted in Moe^G0323 wing discs; large abnormal protrusions replace the microvillar projections seen in wild-type cells.

(Speck et al., 2003)

28% of embryos from Moe^EP1652/Moe^G0323 mothers lack germ cells. Only 1% of embryos from Moe^GT193/Moe^G0323 mothers lack germ cells.

(Jankovics et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Moe^G0323 has lethal | recessive | larval stage phenotype, suppressible by Rho1^72R

(Speck et al., 2003)

Moe^G0323 has lethal | recessive | larval stage phenotype, suppressible by Rho1^E3.10

(Speck et al., 2003)

Moe^G0323 has lethal | recessive | larval stage phenotype, suppressible by Rok¹

(Speck et al., 2003)

Moe^G0323 has lethal | recessive | larval stage phenotype, suppressible by Rok²

(Speck et al., 2003)

Moe^G0323 has lethal | recessive | larval stage phenotype, suppressible by zip²

(Speck et al., 2003)

Moe^G0323 has lethal | recessive | larval stage phenotype, suppressible by zip^Ebr

(Speck et al., 2003)

Enhancer of

Statement

Reference

Moe^G0323/Moe[+] is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of wgn^e00637

(Ruan et al., 2013)

Suppressor of

Statement

Reference

Moe^G0323/Moe[+] is a suppressor of visible phenotype of dsh¹

(Speck et al., 2003)

Phenotype Manifest In

Suppressed by

Statement

Reference

Moe^G0323 has imaginal disc phenotype, suppressible by Rho1^72R

(Speck et al., 2003)

Enhancer of

Statement

Reference

Moe^G0323/Moe[+] is an enhancer of lamina plexus | third instar larval stage phenotype of wgn^e00637

(Ruan et al., 2013)

Moe^G0323/Moe[+] is an enhancer of medulla | third instar larval stage phenotype of wgn^e00637

(Ruan et al., 2013)

Suppressor of

Statement

Reference

Moe^G0323/Moe[+] is a suppressor of terminal tracheal cell | somatic clone phenotype of Syx7^brd1615

(Schottenfeld-Roames et al., 2014)

Moe^G0323/Moe[+] is a suppressor of wing hair | increased number phenotype of dsh¹

(Speck et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity for Moe^G0323 dramatically rescues the Syx7^brd1615 terminal tracheal cell defects. The number of cysts is reduced, and terminal branching is greatly restored. The early endocytosis defects of Syx7^brd1615 are not rescued by heterozygous Moe^G0323.

(Schottenfeld-Roames et al., 2014)

Moe^G0323/+ increases the penetrance of the axonal mistargeting defects seen in the lamina and medulla of hemizygous wgn^e00637 third instar larvae from 70% to 100%.

(Ruan et al., 2013)

Both hemizygous Moe^G0323; Rho1^72R/+ and homozygous Moe^G0323; Rho1^72R/+ adult retinas have normal photoreceptors.

(Karagiosis and Ready, 2004)

Halving the maternal and zygotic dose of Rho1⁺ (using Rho1^72R) strongly suppresses the lethality of Moe^G0323 animals and also suppresses the Moe^G0323 disc epithelium organisation and actin localisation phenotypes.

(Speck et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Moe^G0323 is rescued by Moe^{T559D.UAS.Tag:MYC}/Scer\GAL4^T80

(Speck et al., 2003)

Moe^G0323 is rescued by Scer\GAL4^T80/Moe^UAS.Tag:MYC

(Speck et al., 2003)

Partially rescued by

Moe^G0323 is partially rescued by Moe^{K.UAS.Tag:MYC}/Scer\GAL4^sca-109-68

(Ruan et al., 2013)

Moe^G0323 is partially rescued by Scer\GAL4^arm.PS/Moe^TD.UAS.EGFP

(Hipfner et al., 2004)

Moe^G0323 is partially rescued by Scer\GAL4^en-e16E/Moe^UAS.Tag:MYC

(Speck et al., 2003)

Not rescued by

Moe^G0323 is not rescued by Moe^{T559A.UAS.Tag:MYC}/Scer\GAL4^T80

(Speck et al., 2003)

Comments

Expression of Moe^{K.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^sca-109-68 rescues the R2-R5 axon targeting defects seen in Moe^G0323 hemizygotes in 38% of cases.

(Ruan et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

10897

w^67c23 P{lacW}Moe^G0323/FM7c, Bar⁺

Notes on Origin

Discoverer

Comments

P{lacW} not verified as causing the lethality.

(Schäfer et al., 1999.6.15)

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AJ426795

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Dmoe^G0323

(Bajur et al., 2019, Jankovics et al., 2002)

Moe^G0323

(Hughes et al., 2010, Vilmos et al., 2009)

l(1)G0323

(Peter et al., 2002)

l(1)G0323^G0323

(Schäfer et al., 1999.6.15)

moe^G0323

(Vilmos et al., 2016, Ruan et al., 2013, Molnar and de Celis, 2006)

Name Synonyms

Secondary FlyBase IDs

References (16)

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