FlyBase Allele Report: Dmel\Moe[TD.UAS.EGFP]

General Information

Symbol

Dmel\Moe^TD.UAS.EGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0150741

Feature type

allele

Associated gene

Dmel\Moe

Associated Insertion(s)

Carried in Construct

P{UAS-Moe.TD::GFP}

Key Links

Transgenic product class

missense_variant

(Polesello et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Polesello et al., 2002)

Mutagen

in vitro construct

(Polesello et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-Moe.TD::GFP}

Cytology

Description

Expression of mutated full length Moe (carries the amino acid replacement T559D), fused at the C-terminal to EGFP is under the control of UAS sequences.

(Polesello et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Moe

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Moe^PL106, Scer\GAL4^αTub84B.PL

(Roch et al., 2010)

lethal, with Scer\GAL4^αTub84B.PL

(Roch et al., 2010)

Phenotype Manifest In

cyst progenitor cell, with Scer\GAL4^C587

(Cheng et al., 2011)

nurse cell & actin filament, with Scer\GAL4^Moe-PG26

(Polesello et al., 2002)

nurse cell & actin filament, with Scer\GAL4^{VP16.nanos.UTR}

(Polesello et al., 2002)

spindle, with Scer\GAL4^C587

(Cheng et al., 2011)

Detailed Description

Statement

Reference

Only 51% of testis somatic cyst stem cells show repositioning of the mitotic spindle during anaphase in males expressing Moe^{TD.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^C587 compared to 76% of cases in heterozygous controls.

(Cheng et al., 2011)

Expression of Moe^{TD.Scer\UAS.T:Avic\GFP-EGFP} induces lethality in Moe^PL106 escapers and wild-type siblings.

(Roch et al., 2010)

When Moe^{TD.Scer\UAS.T:Avic\GFP-EGFP} is driven by Scer\GAL4^arm.PS in imaginal discs, no effect is seen on the development of the mutant animals.

(Hipfner et al., 2004)

In the nurse cells of Moe^{TD.Scer\UAS.T:Avic\GFP-EGFP}; Scer\GAL4^Moe-PG26 or Moe^{TD.Scer\UAS.T:Avic\GFP-EGFP}; Scer\GAL4^{nos.UTR.T:Hsim\VP16} females, the mesh-like structure of the cortical actin network disappears and actin filaments are concentrated in dense spots in the cortex and lateral membranes. In addition, overall cell shape is altered and nurse cells become abnormally round.

(Polesello et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^arm.PS/Moe^TD.UAS.EGFP is a suppressor of lethal | recessive | pupal stage phenotype of Slik¹

(Hipfner et al., 2004)

NOT Suppressor of

Statement

Reference

Scer\GAL4^arm.PS/Moe^TD.UAS.EGFP is a non-suppressor of abnormal developmental rate phenotype of Slik¹

(Hipfner et al., 2004)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^arm.PS/Moe^TD.UAS.EGFP is a suppressor of wing & epithelial cell | ectopic phenotype of Slik¹

(Hipfner et al., 2004)

Scer\GAL4^arm.PS/Moe^TD.UAS.EGFP is a suppressor of wing & epithelial cell phenotype of Slik¹

(Hipfner et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^arm.PS/Moe^TD.UAS.EGFP partially rescues Moe^G0323

(Hipfner et al., 2004)

Fails to rescue

Scer\GAL4^αTub84B.PL/Moe^TD.UAS.EGFP fails to rescue Moe^PL106

(Roch et al., 2010)

Scer\GAL4^αTub84B.PL/Moe^TD.UAS.EGFP fails to rescue Moe^PL54

(Roch et al., 2010)

Comments

When Moe^{TD.Scer\UAS.T:Avic\GFP-EGFP} is driven by Scer\GAL4^arm.PS in Moe^G0323 mutants, more than half of the mutant larvae are rescued to pharate adult( 25%) or adult stages (28%).

(Hipfner et al., 2004)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Moe^{TD.Scer\UAS.T:Avic\GFP-EGFP}

Moe^TD.UAS.EGFP

Name Synonyms

phosphomimetic Dmoesin-GFP

(Miller, 2003)

Secondary FlyBase IDs

References (7)

Report Sections

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