FlyBase Allele Report: Dmel\Slik[1]

General Information

Symbol

Dmel\Slik¹

Species

D. melanogaster

Name

FlyBase ID

FBal0152299

Feature type

allele

Associated gene

Dmel\Slik

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Hipfner and Cohen, 2003)

Progenitor genotype

P{EPg}Slik²⁰³⁴⁸

(Hipfner and Cohen, 2003)

Cytology

Description

Imprecise excision has created this mutation. Exons 2 to 8 and part of exon 9 have been deleted.

(Hipfner and Cohen, 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell adhesion | embryonic stage 17

(Katzemich et al., 2019)

abnormal cell adhesion | embryonic stage 17 (with Df(2R)BSC603)

(Katzemich et al., 2019)

abnormal developmental rate

(Hipfner et al., 2004)

abnormal neuroanatomy | adult stage

(Ogi et al., 2019)

abnormal neuroanatomy | pupal stage | somatic clone

(Ogi et al., 2019)

abnormal size | adult stage | somatic clone

(Hipfner and Cohen, 2003)

decreased body size, with Scer\GAL4^arm.PS, Slik^UAS.cHa

(Hipfner and Cohen, 2003)

decreased cell number | larval stage | somatic clone

(Hipfner and Cohen, 2003)

increased cell death (with Slik^KG04837)

(Hipfner and Cohen, 2003)

increased cell death | larval stage | somatic clone

(Hipfner and Cohen, 2003)

increased cell death | somatic clone

(Hipfner and Cohen, 2003)

increased cell death | somatic clone (with Slik^KG04837)

(Hipfner and Cohen, 2003)

lethal - all die before end of larval stage, with Scer\GAL4^Mef2.PU, Slik^UAS.cUa

(Katzemich et al., 2019)

lethal - all die before end of pupal stage | recessive

(Hipfner et al., 2004)

lethal - all die during embryonic stage

(Katzemich et al., 2019)

partially lethal - majority die (with Slik^KG04837)

(Hipfner and Cohen, 2003)

some die during embryonic stage, with Scer\GAL4^Mef2.PU, Slik^UAS.cUa

(Katzemich et al., 2019)

some die during pupal stage | recessive

(Hipfner et al., 2004)

Phenotype Manifest In

embryonic somatic muscle cell | embryonic stage 17

(Katzemich et al., 2019)

embryonic somatic muscle cell | embryonic stage 17 (with Df(2R)BSC603)

(Katzemich et al., 2019)

eye photoreceptor cell stalk | adult stage

(Ogi et al., 2019)

eye photoreceptor cell stalk | pupal stage | somatic clone

(Ogi et al., 2019)

ommatidium & microvillus | somatic clone

(Hipfner et al., 2004)

outer photoreceptor cell | pupal stage | somatic clone

(Ogi et al., 2019)

rhabdomere | somatic clone

(Hipfner et al., 2004)

rhabdomere microvillus | adult stage

(Ogi et al., 2019)

rhabdomere of eye photoreceptor cell | adult stage

(Ogi et al., 2019)

rhabdomere of eye photoreceptor cell | pupal stage | somatic clone

(Ogi et al., 2019)

wing (with Slik^KG04837)

(Hipfner and Cohen, 2003)

wing & epithelial cell

(Hipfner et al., 2004)

wing & epithelial cell | ectopic

(Hipfner et al., 2004)

wing | somatic clone (with Slik^KG04837)

(Hipfner and Cohen, 2003)

Detailed Description

Statement

Reference

Slik¹ embryos show muscle detachment at stage 17, but not at stage 16. Stage 17 Slik¹/Df(2R)BSC603 embryos show a similar muscle detachment phenotype.

(Katzemich et al., 2019)

In pupal Slik¹ homozygous mosaic retinas, ommatidia composed of only mutant photoreceptors exhibit C-shaped peripheral photoreceptor rhabdomeres; in adult homozygous photoreceptors, the stalk-rhabdomere segregation is disrupted, catacomb-like membrane architecture is absent and rhabdomeric microvilli do not unite to form a single round structure, instead spread to the entire apical membrane, compared to controls.

(Ogi et al., 2019)

Nearly half of mutant animals survive to pupation but die shortly after. In mutant wings, the columnar epithelium is abnormally thin. Many cells lose their capacity to remain intergrated in the epithelium and are extruded basally to form a disorganised mass. Many of these cells undergo apoptosis, though clusters of these cells survive. When homozygous mutant clones are made in the eye, defects are seen in rhabdomere differentiation. Many mutant ommatidia have patches of apical membrane devoid of microvilli. However mutant photoreceptors are present in the eyes of 18-day old adult flies.

(Hipfner et al., 2004)

slik¹ animals rescued with slik^Scer\UAS.cHa driven by Scer\GAL4^arm.PS develop to adulthood of reduced body size, with mildly rough eyes, but of otherwise normal appearance. Mutant larvae show a decrease in endoreplication. slik¹/slik^KG04837 animals are viable, 90% of expected flies survive. Homozygous mutant clones generated in the wing imaginal disc between about 48 hours cover on average only 44% of the area of the corresponding wild-type twin clones. This is caused by a defect in cell survival. When mutant clones are made in a Minute background at about 60h (+-12h), the wings in the adult are curved upward of downward to varying degrees, suggesting that there are differences in the sizes of the dorsal and ventral surfaces of the wing blade. In more severe cases, the wings are small and contain vesicles of blackened tissue. However mutant cells that remain in the epithelium differentiate into morphologically normal wing blade cells, margin, and wing veins. Mutant cells are the same size as wild-type cells.

(Hipfner and Cohen, 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Slik^KG04837/Slik¹ has partially lethal - majority die phenotype, enhanceable by Raf⁷

(Hipfner and Cohen, 2003)

Slik^KG04837/Slik¹ has increased cell death phenotype, enhanceable by Raf⁷

(Hipfner and Cohen, 2003)

Slik¹ has increased cell death | larval stage | somatic clone phenotype, enhanceable by Raf[+]/Raf⁷

(Hipfner and Cohen, 2003)

Suppressed by

Statement

Reference

Slik¹ has increased mortality during development phenotype, suppressible by rhea^{T152E.UAS.Tag:FLAG}/Scer\GAL4^Mef2.PU

(Katzemich et al., 2019)

Slik¹ has lethal | recessive | pupal stage phenotype, suppressible by Scer\GAL4^arm.PS/Moe^TD.UAS.EGFP

(Hipfner et al., 2004)

Slik¹ has increased cell death | somatic clone phenotype, suppressible by Raf^UAS.F179/Scer\GAL4^αTub84B.PL

(Hipfner and Cohen, 2003)

Slik¹ has increased cell death | somatic clone phenotype, suppressible by hep^r75

(Hipfner and Cohen, 2003)

NOT suppressed by

Statement

Reference

Slik¹ has abnormal developmental rate phenotype, non-suppressible by Scer\GAL4^arm.PS/Moe^TD.UAS.EGFP

(Hipfner et al., 2004)

Slik¹ has increased cell death | somatic clone phenotype, non-suppressible by rl^Sem.cUa.UAS/Scer\GAL4^αTub84B.PL

(Hipfner and Cohen, 2003)

Other

Statement

Reference

Scer\GAL4^Mef2.PU, Slik¹, rhea^{T152E.UAS.Tag:FLAG} has lethal - all die before end of larval stage phenotype

(Katzemich et al., 2019)

Slik¹/Df(2R)BSC603, Scer\GAL4^Mef2.PU, rhea^{T152E.UAS.Tag:FLAG} has some die during embryonic stage phenotype

(Katzemich et al., 2019)

Phenotype Manifest In

Enhanced by

Statement

Reference

Slik^KG04837/Slik¹ has wing phenotype, enhanceable by Raf⁷

(Hipfner and Cohen, 2003)

Suppressed by

Statement

Reference

Slik¹ has wing & epithelial cell | ectopic phenotype, suppressible by Scer\GAL4^arm.PS/Moe^TD.UAS.EGFP

(Hipfner et al., 2004)

Slik¹ has wing & epithelial cell phenotype, suppressible by Scer\GAL4^arm.PS/Moe^TD.UAS.EGFP

(Hipfner et al., 2004)

Enhancer of

Statement

Reference

slik[+]/Slik¹ is an enhancer of wing phenotype of Mer^{1-600.UAS.Tag:MYC}, Scer\GAL4^ap-md544

(Hughes and Fehon, 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Reduction of slik in a slik¹/+ background reduces wing size by a mean of 18% in flies expressing Mer^{1-600.Scer\UAS.T:Hsap\MYC} (under the control of Scer\GAL4^ap-md544) compared to wild-type flies (and compared to a 15% reduction in

(Hughes and Fehon, 2006)

9% of slik¹ mutants expressing Moe^{TD.Scer\UAS.T:Avic\GFP-EGFP} driven by Scer\GAL4^arm.PS, reach pharate adult stages, compared top none in slik¹ alone. Imaginal discs from these animals show improvement in overall structure and organisation, basal extrusion of cells is reduced and in some cases nearly eliminated.

(Hipfner et al., 2004)

When heterozygous phl⁷ is added to slik¹/slik^KG04837 animals about 40% of animals survive(as opposed to 90%) and the penetrance of the wing phenotype is increased from 11% to almost 100%. The number of apoptotic cells seen also increases.

(Hipfner and Cohen, 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Slik¹ is partially rescued by Slik^UAS.cUa/Scer\GAL4^Mef2.PU

(Katzemich et al., 2019)

Slik¹ is partially rescued by Scer\GAL4^arm.PS/Slik^UAS.cHa

(Hipfner and Cohen, 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

600519

w^*; P{neoFRT}42D Slik¹/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Plkk1¹

Slik¹

(Katzemich et al., 2019, Ogi et al., 2019)

slik¹

(Hughes et al., 2010, Hughes and Fehon, 2006)

Name Synonyms

Secondary FlyBase IDs

References (6)

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