FlyBase Allele Report: Dmel\Moe[PL54]

General Information

Symbol

Dmel\Moe^PL54

Species

D. melanogaster

Name

FlyBase ID

FBal0130903

Feature type

allele

Associated gene

Dmel\Moe

Associated Insertion(s)

P{lacW}Moe^PL54

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Claret et al., 2014)

Mutagen

Delta2-3 transposase

(Bourbon et al., 2002)

Progenitor genotype

Associated Insertion(s)

P{lacW}Moe^PL54

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage

(Ogi et al., 2019)

abnormal neuroanatomy | pupal stage | somatic clone

(Ogi et al., 2019)

lethal | embryonic stage

(Roch et al., 2010)

lethal | larval stage

(Roch et al., 2010)

lethal | recessive

(Bourbon et al., 2002)

Phenotype Manifest In

embryonic/first instar larval cuticle | non-rescuable maternal effect

(Polesello et al., 2002)

embryonic abdominal segment | non-rescuable maternal effect

(Polesello et al., 2002)

eye photoreceptor cell stalk | adult stage

(Ogi et al., 2019)

outer photoreceptor cell | pupal stage | somatic clone

(Ogi et al., 2019)

primordial germ cell | non-rescuable maternal effect

(Polesello et al., 2002)

rhabdomere & actin filament | somatic clone

(Karagiosis and Ready, 2004)

rhabdomere & plasma membrane | apical | somatic clone

(Karagiosis and Ready, 2004)

rhabdomere microvillus | adult stage

(Ogi et al., 2019)

rhabdomere of eye photoreceptor cell | adult stage

(Ogi et al., 2019)

rhabdomere of eye photoreceptor cell | pupal stage | somatic clone

(Ogi et al., 2019)

Detailed Description

Statement

Reference

In pupal Moe^PL54 homozygous mosaic retinas, ommatidia composed of only mutant photoreceptors exhibit C-shaped peripheral photoreceptor rhabdomeres; in adult homozygous photoreceptors, the stalk-rhabdomere segregation is disrupted, catacomb-like membrane architecture is absent and rhabdomeric microvilli do not unite to form a single round structure, instead spread to the entire apical membrane, compared to controls.

(Ogi et al., 2019)

Moe^PL54 leads to 100% lethality in embryonic or early larval stages.

(Roch et al., 2010)

The apical membranes of photoreceptor rhabdomeres are severely disrupted in Moe^PL54 homozygous somatic clones. The rhabdomere microvillar array is irregular in these cells: the rhabdomere terminal web is replaced by abnormal F-actin accumulations.

(Karagiosis and Ready, 2004)

A proportion of the eggs laid by heterozygous Moe^PL54 heterozygous females display segmentation defects (8%, n=64). These abnormal embryos lack posterior structures.

(Polesello et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Moe^PL54 has abnormal neuroanatomy | pupal stage | somatic clone phenotype, non-enhanceable by Synd^661T

(Ogi et al., 2019)

Moe^PL54 has abnormal neuroanatomy | adult stage phenotype, non-enhanceable by Synd^661T

(Ogi et al., 2019)

NOT suppressed by

Statement

Reference

Moe^PL54 has abnormal neuroanatomy | somatic clone | pupal stage phenotype, non-suppressible by Synd^661T

(Ogi et al., 2019)

Moe^PL54 has abnormal neuroanatomy | adult stage phenotype, non-suppressible by Synd^661T

(Ogi et al., 2019)

NOT Enhancer of

Statement

Reference

Moe^PL54/Moe^PL54 is a non-enhancer of abnormal neuroanatomy | pupal stage | somatic clone phenotype of Synd^661T

(Ogi et al., 2019)

Moe^PL54/Moe^PL54 is a non-enhancer of abnormal neuroanatomy | adult stage phenotype of Synd^661T

(Ogi et al., 2019)

NOT Suppressor of

Statement

Reference

Moe^PL54/Moe^PL54 is a non-suppressor of abnormal neuroanatomy | somatic clone | pupal stage phenotype of Synd^661T

(Ogi et al., 2019)

Moe^PL54/Moe^PL54 is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of Synd^661T

(Ogi et al., 2019)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Moe^PL54 has rhabdomere of eye photoreceptor cell | pupal stage | somatic clone phenotype, non-enhanceable by Synd^661T

(Ogi et al., 2019)

Moe^PL54 has eye photoreceptor cell stalk | adult stage phenotype, non-enhanceable by Synd^661T

(Ogi et al., 2019)

Moe^PL54 has rhabdomere of eye photoreceptor cell | adult stage phenotype, non-enhanceable by Synd^661T

(Ogi et al., 2019)

Moe^PL54 has eye photoreceptor cell stalk | pupal stage | somatic clone phenotype, non-enhanceable by Synd^661T

(Ogi et al., 2019)

Moe^PL54 has outer photoreceptor cell | pupal stage | somatic clone phenotype, non-enhanceable by Synd^661T

(Ogi et al., 2019)

Moe^PL54 has outer photoreceptor cell | adult stage phenotype, non-enhanceable by Synd^661T

(Ogi et al., 2019)

NOT suppressed by

Statement

Reference

Moe^PL54 has rhabdomere of eye photoreceptor cell | somatic clone | pupal stage phenotype, non-suppressible by Synd^661T

(Ogi et al., 2019)

Moe^PL54 has eye photoreceptor cell stalk | adult stage phenotype, non-suppressible by Synd^661T

(Ogi et al., 2019)

Moe^PL54 has rhabdomere of eye photoreceptor cell | adult stage phenotype, non-suppressible by Synd^661T

(Ogi et al., 2019)

Moe^PL54 has eye photoreceptor cell stalk | somatic clone | pupal stage phenotype, non-suppressible by Synd^661T

(Ogi et al., 2019)

Moe^PL54 has outer photoreceptor cell | somatic clone | pupal stage phenotype, non-suppressible by Synd^661T

(Ogi et al., 2019)

Moe^PL54 has outer photoreceptor cell | adult stage phenotype, non-suppressible by Synd^661T

(Ogi et al., 2019)

NOT Enhancer of

Statement

Reference

Moe^PL54/Moe^PL54 is a non-enhancer of rhabdomere of eye photoreceptor cell | pupal stage | somatic clone phenotype of Synd^661T

(Ogi et al., 2019)

Moe^PL54/Moe^PL54 is a non-enhancer of eye photoreceptor cell stalk | adult stage phenotype of Synd^661T

(Ogi et al., 2019)

Moe^PL54/Moe^PL54 is a non-enhancer of rhabdomere of eye photoreceptor cell | adult stage phenotype of Synd^661T

(Ogi et al., 2019)

Moe^PL54/Moe^PL54 is a non-enhancer of eye photoreceptor cell stalk | pupal stage | somatic clone phenotype of Synd^661T

(Ogi et al., 2019)

Moe^PL54/Moe^PL54 is a non-enhancer of outer photoreceptor cell | pupal stage | somatic clone phenotype of Synd^661T

(Ogi et al., 2019)

Moe^PL54/Moe^PL54 is a non-enhancer of outer photoreceptor cell | adult stage phenotype of Synd^661T

(Ogi et al., 2019)

NOT Suppressor of

Statement

Reference

Moe^PL54/Moe^PL54 is a non-suppressor of rhabdomere of eye photoreceptor cell | somatic clone | pupal stage phenotype of Synd^661T

(Ogi et al., 2019)

Moe^PL54/Moe^PL54 is a non-suppressor of eye photoreceptor cell stalk | adult stage phenotype of Synd^661T

(Ogi et al., 2019)

Moe^PL54/Moe^PL54 is a non-suppressor of rhabdomere of eye photoreceptor cell | adult stage phenotype of Synd^661T

(Ogi et al., 2019)

Moe^PL54/Moe^PL54 is a non-suppressor of eye photoreceptor cell stalk | somatic clone | pupal stage phenotype of Synd^661T

(Ogi et al., 2019)

Moe^PL54/Moe^PL54 is a non-suppressor of outer photoreceptor cell | somatic clone | pupal stage phenotype of Synd^661T

(Ogi et al., 2019)

Moe^PL54/Moe^PL54 is a non-suppressor of outer photoreceptor cell | adult stage phenotype of Synd^661T

(Ogi et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

In pupal and adult Moe^PL54 mosaic retinas on a Synd^661T/Df(3R)BSC43 background the extent of stalk-rhabdomere segregation defect is not different than Synd^661T homozygous mosaic retinas.

(Ogi et al., 2019)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Moe^PL54 is rescued by Moe^WT.UAS.EGFP/Scer\GAL4^αTub84B.PL

(Roch et al., 2010)

Moe^PL54 is rescued by Scer\GAL4^αTub84B.PL/Moe^UASp.cRa

(Roch et al., 2010)

Partially rescued by

Moe^PL54 is partially rescued by Moe^TA.UASp/Scer\GAL4^αTub84B.PL