FlyBase Allele Report: Dmel\Shrm[A.UAS]

General Information

Symbol

Dmel\Shrm^A.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0248401

Feature type

allele

Associated gene

Dmel\Shrm

Associated Insertion(s)

Carried in Construct

P{UAS-Shrm.A}

Key Links

Transgenic product class

wild_type

(Bolinger et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Bolinger et al., 2010)

Mutagen

in vitro construct

(Bolinger et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-Shrm.A}

Cytology

Description

UASt regulatory sequences drive expression of Shroom isoform-A coding sequences.

(Bolinger et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Shrm

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

apical part of cell | larval stage, with Scer\GAL4^A9

(Hildebrand et al., 2021)

dorsal ectoderm, with Scer\GAL4^arm.PS

(Bolinger et al., 2010)

dorsal ectoderm, with Scer\GAL4^pnr-MD237

(Bolinger et al., 2010)

epithelial cell, with Scer\GAL4^arm.PS

(Bolinger et al., 2010)

epithelial cell, with Scer\GAL4^pnr-MD237

(Bolinger et al., 2010)

epithelial cell of wing disc, with Scer\GAL4^A9

(Hildebrand et al., 2021)

eye, with Scer\GAL4^lz-gal4

(Hildebrand et al., 2021)

eye disc, with Scer\GAL4^lz-gal4

(Hildebrand et al., 2021)

ommatidium | larval stage, with Scer\GAL4^lz-gal4

(Hildebrand et al., 2021)

ommatidium | pupal stage, with Scer\GAL4^lz-gal4

(Hildebrand et al., 2021)

photoreceptor | pupal stage, with Scer\GAL4^lz-gal4

(Hildebrand et al., 2021)

tracheal lumen | embryonic stage, with Scer\GAL4^btl.PS

(Beati et al., 2018)

wing, with Scer\GAL4^A9

(Hildebrand et al., 2021)

wing disc, with Scer\GAL4^A9

(Hildebrand et al., 2021)

wing disc ventral compartment, with Scer\GAL4^A9

(Hildebrand et al., 2021)

Detailed Description

Statement

Reference

The expression of Shrm^A.UAS under the control of Scer\GAL4^A9 induces a small wing which show dorsal curling; the wing disc is distorted and cells in the ventral portion exhibit a smaller apical area. Expression under the control of Scer\GAL4^lz-gal4 induces a small, rough eye phenotype; in the larval eye disc, cells are apically-constricted and some ommatidia are irregular in shape; in the pupal eye disc, photoreceptors are disorganized in the ommatidium.

(Hildebrand et al., 2021)

Expression of Shroom^A.UAS under the control of Scer\GAL4^btl.PS causes a significant reduction of the tracheal lumen diameter in the embryo, compared to controls.

(Beati et al., 2018)

Expression of Shroom^A.Scer\UAS under the control of Scer\GAL4^arm.PS results in embryonic lethality due to multiple defects in morphogenesis. This appears to results from excessive apical constriction and subsequent disruption of the adherens junction in the embryonic ectoderm.

Overexpression of Shroom^A.Scer\UAS in the dorsal ectoderm under the control of Scer\GAL4^pnr-MD237 causes embryonic lethality due to defects in dorsal closure. This correlates with the inability of epidermal cells within the Scer\GAL4^pnr-MD237 expression domain, including the leading edge cells, to elongate along the dorsal-ventral axis. These cells also appear to have a more rounded apical surface, a smaller circumference and to be more disorganised than those in control embryos. Shroom^A.Scer\UAS expression causes a roughly 70% decrease in apical area.

(Bolinger et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^A9, Shrm^A.UAS has decreased size | adult stage phenotype, enhanceable by flw[+]/flw⁶

(Hildebrand et al., 2021)

Scer\GAL4^lz-gal4, Shrm^A.UAS has visible | adult stage phenotype, enhanceable by Egfr[+]/Egfr^f2

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has visible | adult stage phenotype, enhanceable by flw[+]/flw⁶

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has visible | adult stage phenotype, enhanceable by Rap1[+]/Rap1^R-1

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has visible | adult stage phenotype, enhanceable by Src42A^k10108/Src42A[+]

(Hildebrand et al., 2021)

Scer\GAL4^lz-gal4, Shrm^A.UAS has visible | adult stage phenotype, enhanceable by Src42A^k10108/Src42A[+]

(Hildebrand et al., 2021)

Suppressed by

Statement

Reference

Scer\GAL4^A9, Shrm^A.UAS has visible | adult stage phenotype, suppressible | partially by zip[+]/zip¹

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has decreased size | adult stage phenotype, suppressible | partially by zip[+]/zip¹

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has visible | adult stage phenotype, suppressible | partially by zip[+]/zip⁰²⁹⁵⁷

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has visible | adult stage phenotype, suppressible | partially by zip²/zip[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has decreased size | adult stage phenotype, suppressible | partially by zip²/zip[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has decreased size | adult stage phenotype, suppressible | partially by zip[+]/zip⁰²⁹⁵⁷

(Hildebrand et al., 2021)

Scer\GAL4^lz-gal4, Shrm^A.UAS has visible | adult stage phenotype, suppressible by zip[+]/zip¹

(Hildebrand et al., 2021)

Scer\GAL4^lz-gal4, Shrm^A.UAS has visible | adult stage phenotype, suppressible by zip²/zip[+]

(Hildebrand et al., 2021)

Scer\GAL4^lz-gal4, Shrm^A.UAS has visible | adult stage phenotype, suppressible by zip[+]/zip⁰²⁹⁵⁷

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has decreased size | adult stage phenotype, suppressible | partially by Rok[+]/Rok¹

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has decreased size | adult stage phenotype, suppressible | partially by sqh^AX3/sqh[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has decreased size | adult stage phenotype, suppressible | partially by Rok²/Rok[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has visible | adult stage phenotype, suppressible | partially by Rok[+]/Rok¹

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has visible | adult stage phenotype, suppressible | partially by sqh^AX3/sqh[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has visible | adult stage phenotype, suppressible | partially by Rok²/Rok[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has visible | adult stage phenotype, suppressible | partially by chic[+]/chic²²¹

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has visible | adult stage phenotype, suppressible | partially by jar²⁰⁹⁵/jar[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has visible | adult stage phenotype, suppressible | partially by ena²³/ena[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has visible | adult stage phenotype, suppressible | partially by shot^SF20/shot[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has visible | adult stage phenotype, suppressible | partially by shot^V104/shot[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has visible | adult stage phenotype, suppressible | partially by ena[+]/ena⁴⁶

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has visible | adult stage phenotype, suppressible | partially by dia[+]/dia⁵

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has visible | adult stage phenotype, suppressible | partially by Eb1⁰⁴⁵²⁴/Eb1[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has visible | adult stage phenotype, suppressible | partially by trio^6A/trio[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has visible | adult stage phenotype, suppressible | partially by shot³/shot[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has visible | adult stage phenotype, suppressible | partially by ssh^1-63/ssh[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has decreased size | adult stage phenotype, suppressible | partially by chic[+]/chic²²¹

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has decreased size | adult stage phenotype, suppressible | partially by dia[+]/dia⁵

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has decreased size | adult stage phenotype, suppressible | partially by ena[+]/ena⁴⁶

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has decreased size | adult stage phenotype, suppressible | partially by ena²³/ena[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has decreased size | adult stage phenotype, suppressible | partially by Tm1[+]/Tm1^Su(flw)4

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has decreased size | adult stage phenotype, suppressible | partially by jar²⁰⁹⁵/jar[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has decreased size | adult stage phenotype, suppressible | partially by Eb1⁰⁴⁵²⁴/Eb1[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has decreased size | adult stage phenotype, suppressible | partially by trio^6A/trio[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has decreased size | adult stage phenotype, suppressible | partially by shot³/shot[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has decreased size | adult stage phenotype, suppressible | partially by shot^SF20/shot[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has decreased size | adult stage phenotype, suppressible | partially by shot^V104/shot[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has decreased size | adult stage phenotype, suppressible | partially by ssh^1-63/ssh[+]

(Hildebrand et al., 2021)

Scer\GAL4^lz-gal4, Shrm^A.UAS has visible | adult stage phenotype, suppressible | partially by ena²³/ena[+]

(Hildebrand et al., 2021)

Scer\GAL4^lz-gal4, Shrm^A.UAS has decreased size | adult stage phenotype, suppressible | partially by ena²³/ena[+]

(Hildebrand et al., 2021)

Scer\GAL4^lz-gal4, Shrm^A.UAS has decreased size | adult stage phenotype, suppressible | partially by ena[+]/ena⁴⁶

(Hildebrand et al., 2021)

Scer\GAL4^lz-gal4, Shrm^A.UAS has visible | adult stage phenotype, suppressible | partially by ena[+]/ena⁴⁶

(Hildebrand et al., 2021)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^A9, Shrm^A.UAS has wing phenotype, enhanceable by flw[+]/flw⁶

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has wing phenotype, enhanceable by Rap1[+]/Rap1^R-1

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has wing phenotype, enhanceable by Src42A^k10108/Src42A[+]

(Hildebrand et al., 2021)

Scer\GAL4^lz-gal4, Shrm^A.UAS has wing phenotype, enhanceable by Src42A^k10108/Src42A[+]

(Hildebrand et al., 2021)

Scer\GAL4^lz-gal4, Shrm^A.UAS has wing phenotype, enhanceable by Egfr[+]/Egfr^f2

(Hildebrand et al., 2021)

Suppressed by

Statement

Reference

Scer\GAL4^A9, Shrm^A.UAS has wing phenotype, suppressible | partially by zip²/zip[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has wing phenotype, suppressible | partially by sqh^AX3/sqh[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has wing phenotype, suppressible | partially by zip[+]/zip⁰²⁹⁵⁷

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has wing phenotype, suppressible | partially by chic[+]/chic²²¹

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has wing phenotype, suppressible | partially by ena[+]/ena⁴⁶

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has wing phenotype, suppressible | partially by Eb1⁰⁴⁵²⁴/Eb1[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has wing phenotype, suppressible | partially by ssh^1-63/ssh[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has wing phenotype, suppressible | partially by jar²⁰⁹⁵/jar[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has wing phenotype, suppressible | partially by trio^6A/trio[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has wing phenotype, suppressible | partially by Tm1[+]/Tm1^Su(flw)4

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has wing phenotype, suppressible | partially by dia[+]/dia⁵

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has wing phenotype, suppressible | partially by ena²³/ena[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has wing phenotype, suppressible | partially by shot^SF20/shot[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has wing phenotype, suppressible | partially by shot³/shot[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has wing phenotype, suppressible | partially by shot^V104/shot[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has wing disc ventral compartment phenotype, suppressible | partially by ena[+]/ena⁴⁶

(Hildebrand et al., 2021)

Scer\GAL4^lz-gal4, Shrm^A.UAS has eye phenotype, suppressible | partially by ena²³/ena[+]

(Hildebrand et al., 2021)

Scer\GAL4^lz-gal4, Shrm^A.UAS has eye phenotype, suppressible | partially by ena[+]/ena⁴⁶

(Hildebrand et al., 2021)

Scer\GAL4^lz-gal4, Shrm^A.UAS has eye phenotype, suppressible by zip[+]/zip⁰²⁹⁵⁷

(Hildebrand et al., 2021)

Scer\GAL4^lz-gal4, Shrm^A.UAS has eye phenotype, suppressible by zip[+]/zip¹

(Hildebrand et al., 2021)

Scer\GAL4^lz-gal4, Shrm^A.UAS has eye phenotype, suppressible by zip²/zip[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has wing phenotype, suppressible | partially by Rok[+]/Rok¹

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has wing phenotype, suppressible | partially by Rok²/Rok[+]

(Hildebrand et al., 2021)

Scer\GAL4^A9, Shrm^A.UAS has wing phenotype, suppressible | partially by zip[+]/zip¹

(Hildebrand et al., 2021)

Scer\GAL4^pnr-MD237, Shrm^A.UAS has epithelial cell phenotype, suppressible by Rok^{SBD.UAS.Tag:MYC}, Scer\GAL4^pnr-MD237

(Bolinger et al., 2010)

Scer\GAL4^pnr-MD237, Shrm^A.UAS has dorsal ectoderm phenotype, suppressible by Rok^{SBD.UAS.Tag:MYC}, Scer\GAL4^pnr-MD237

(Bolinger et al., 2010)

Scer\GAL4^pnr-MD237, Shrm^A.UAS has epithelial cell phenotype, suppressible by zip^ΔHD.UAS.YFP/Scer\GAL4^pnr-MD237

(Bolinger et al., 2010)

Scer\GAL4^pnr-MD237, Shrm^A.UAS has dorsal ectoderm phenotype, suppressible by zip^ΔHD.UAS.YFP/Scer\GAL4^pnr-MD237

(Bolinger et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of rok^{SBD.Scer\UAS.T:Hsap\MYC} with Shroom^A.Scer\UAS, both under the control of Scer\GAL4^pnr-MD237 blocks the epithelial cell apical constriction seen in Shroom^A.Scer\UAS single mutants.

Co-expression of zip^{ΔHD.Scer\UAS.T:Avic\GFP-YFP} with Shroom^A.Scer\UAS, both under the control of Scer\GAL4^pnr-MD237 blocks the epithelial cell apical constriction seen in Shroom^A.Scer\UAS single mutants.

(Bolinger et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

59019

P{UAS-Shrm.A}1, w^*

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Shrm^A.UAS

Shroom^A.Scer\UAS

Shroom^A.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

Open Close