FlyBase Allele Report: Dmel\Eb1[04524]

(Hahn et al., 2021, Hildebrand et al., 2021, Bulgakova et al., 2013, Elliott et al., 2005, Beaton, 2000.1.31, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

AF006645

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior (with Eb1²)

(Elliott et al., 2005)

abnormal neuroanatomy

(Alves-Silva et al., 2012)

abnormal neurophysiology

(Elliott et al., 2005)

decreased size | embryonic stage

(Hahn et al., 2021)

female fertile (with Eb1²)

(Baskar et al., 2019)

flightless (with Eb1²)

(Elliott et al., 2005)

flightless (with Eb1⁵)

(Elliott et al., 2005)

lethal | recessive

(Beaton, 2000.1.31, Spradling et al., 1999)

lethal - all die before end of P-stage

(Elliott et al., 2005)

partially lethal (with Eb1²)

(Baskar et al., 2019)

partially lethal - majority die

partially lethal - majority die (with Eb1²)

(Elliott et al., 2005)

partially lethal - majority die (with Eb1⁵)

(Elliott et al., 2005)

partially lethal - majority die (with Eb1^42S11)

(Brumby et al., 2004)

some die during pharate adult stage

(Elliott et al., 2005)

uncoordinated

(Elliott et al., 2005)

visible (with Eb1²)

(Elliott et al., 2005)

visible (with Eb1⁵)

(Elliott et al., 2005)

Phenotype Manifest In

axon

(Alves-Silva et al., 2012)

axon | embryonic stage

(Hahn et al., 2021)

axon microtubule bundle | embryonic stage

(Hahn et al., 2021)

Johnston organ

(Elliott et al., 2005)

microtubule

(Alves-Silva et al., 2012)

microtubule plus-end | embryonic stage

(Hahn et al., 2021)

Wheeler's organ

(Elliott et al., 2005)

wing (with Eb1²)

(Elliott et al., 2005)

wing (with Eb1⁵)

(Elliott et al., 2005)

Detailed Description

Statement

Reference

Eb1⁰⁴⁵²⁴/Eb1² escaper females are fertile.

(Baskar et al., 2019)

Eb1⁰⁴⁵²⁴/Eb1⁰⁴⁵²⁴ embryonic neurons in primary culture show a significant shortening of axons compared to controls and often have patches of non-coalescent, criss-crossed microtubules.

(Alves-Silva et al., 2012)

Eb1⁰⁴⁵²⁴ homozygous larvae show no defects in motility and touch response, show normal imaginal discs and internal organs and have a similar mitotic index and and frequency of anaphase to wild-type larvae. Larvae are able to pupate and develop into pharate adults but fail to eclose. Pharate adults do not show morphological defects but show body coordination defects when dissected from the pupal case. Rare homozygotes are able to eclose and show severe problems in coordinating body movements; their legs shake and often legs of both sides cross over each other, they are barely able to walk and are unable to right themselves after falling upside down.

All Eb1⁵/Eb1⁰⁴⁵²⁴ viable escapers hold their wings in an abnormal 'downward' position and are flightless. Eb1²/Eb1⁰⁴⁵²⁴ flies also show this phenotype but at a lower frequency, with some of flies of this genotype showing held-up or parted wings. All Eb1²/Eb1⁰⁴⁵²⁴ flies are flightless, whether or not they hold their wings in a normal position.

Although Eb1²/Eb1⁰⁴⁵²⁴ flies walk in a coordinated way, once turned upside-down, they fail to recover the correct body position as promptly as wild-type flies; mutant flies often take more than two minutes to right themselves while recovery in wild type is less than a second.

The sound-evoked compound potentials from the Johnston's organ of Eb1⁰⁴⁵²⁴ flies exposed to near-field sound pulse trains are reduced compared to wild-type controls (237μV vs 615μV). This electrophysiological phenotype is not relative to the amount of uncoordination the mutants show.

Eb1⁰⁴⁵²⁴ chordotonal organs of the first abdominal segment display all the basic inner structures with normal morphology and show normal cell division and cell fate determination. However, the alignment and structural integrity of neuronal sensory processes is disrupted in these organs.

(Elliott et al., 2005)

Eb1^42S11/Eb1⁰⁴⁵²⁴ adult escapers do not have any gross abnormalities but generally die within a few days of eclosing, and female escapers are sterile.

(Brumby et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Eb1⁰⁴⁵²⁴/Eb1[+] is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

Eb1⁰⁴⁵²⁴/Eb1[+] is a suppressor | partially of decreased size | adult stage phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

Eb1⁰⁴⁵²⁴/Eb1[+] is a suppressor of decreased occurrence of cell division phenotype of CycE^JP

(Brumby et al., 2004)

Other

Statement

Reference

Eb1⁰⁴⁵²⁴, msps^A, tau^KO has decreased size | larval stage phenotype

(Hahn et al., 2021)

Eb1⁰⁴⁵²⁴, msps^A has decreased size | embryonic stage phenotype

(Hahn et al., 2021)

Eb1⁰⁴⁵²⁴, tau^KO has decreased size | embryonic stage phenotype

(Hahn et al., 2021)

Eb1⁰⁴⁵²⁴, msps^A has decreased size | larval stage phenotype

(Hahn et al., 2021)

Eb1⁰⁴⁵²⁴, shot^unspecified/shot[+] has abnormal neuroanatomy phenotype

(Alves-Silva et al., 2012)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Eb1⁰⁴⁵²⁴ has axon microtubule bundle | embryonic stage phenotype, non-enhanceable by shot³/shot³

(Hahn et al., 2021)

Suppressed by

Statement

Reference

Eb1⁰⁴⁵²⁴, msps^A, tau^KO has axon microtubule bundle | larval stage phenotype, suppressible by shot^LA.UAS.GFP/Scer\GAL4^elav.PLu

(Hahn et al., 2021)

NOT suppressed by

Statement

Reference

Eb1⁰⁴⁵²⁴, msps^A, tau^KO has axon microtubule bundle | larval stage phenotype, non-suppressible by shot^{RE-ΔABD.UAS.GFP}/Scer\GAL4^elav.PLu

(Hahn et al., 2021)

Eb1⁰⁴⁵²⁴, msps^A, tau^KO has axon microtubule bundle | larval stage phenotype, non-suppressible by shot^{RE-3MtLSmut.UAS.GFP}/Scer\GAL4^elav.PLu

(Hahn et al., 2021)

NOT Enhancer of

Statement

Reference

Eb1⁰⁴⁵²⁴/Eb1⁰⁴⁵²⁴ is a non-enhancer of axon microtubule bundle | embryonic stage phenotype of shot³

(Hahn et al., 2021)

Suppressor of

Statement

Reference

Eb1⁰⁴⁵²⁴/Eb1[+] is a suppressor | partially of wing phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

Eb1⁰⁴⁵²⁴/Eb1[+] is a suppressor of eye disc & S phase phenotype of CycE^JP

(Brumby et al., 2004)

Other

Statement

Reference

Eb1⁰⁴⁵²⁴, msps^A, tau^KO has axon microtubule bundle | adult stage phenotype

(Hahn et al., 2021)

Eb1⁰⁴⁵²⁴, msps^A, tau^KO has microtubule plus-end | embryonic stage phenotype

(Hahn et al., 2021)

Eb1⁰⁴⁵²⁴, msps^A, tau^KO has axon microtubule bundle | larval stage phenotype

(Hahn et al., 2021)

Eb1⁰⁴⁵²⁴, msps^A, tau^KO has microtubule plus-end | larval stage phenotype

(Hahn et al., 2021)

Eb1⁰⁴⁵²⁴, msps^A has axon | embryonic stage phenotype

(Hahn et al., 2021)

Eb1⁰⁴⁵²⁴, msps^A has axon microtubule bundle | embryonic stage phenotype

(Hahn et al., 2021)

Eb1⁰⁴⁵²⁴, tau^KO has axon | embryonic stage phenotype

(Hahn et al., 2021)

Eb1⁰⁴⁵²⁴, tau^KO has axon microtubule bundle | embryonic stage phenotype

(Hahn et al., 2021)

Eb1⁰⁴⁵²⁴, msps^A has axon | larval stage phenotype

(Hahn et al., 2021)

Eb1⁰⁴⁵²⁴, msps^A has axon microtubule bundle | larval stage phenotype

(Hahn et al., 2021)

Eb1⁰⁴⁵²⁴, msps^A has microtubule plus-end | larval stage phenotype

(Hahn et al., 2021)

Eb1⁰⁴⁵²⁴, msps^A, tau^KO has axon | larval stage phenotype

(Hahn et al., 2021)

Eb1⁰⁴⁵²⁴, msps^A, tau^KO has medulla | adult stage phenotype

(Hahn et al., 2021)

Eb1⁰⁴⁵²⁴, msps^A, tau^KO has axon | adult stage phenotype

(Hahn et al., 2021)

Eb1⁰⁴⁵²⁴, shot^unspecified/shot[+] has axon phenotype

(Alves-Silva et al., 2012)

Eb1⁰⁴⁵²⁴, shot^unspecified/shot[+] has microtubule phenotype

(Alves-Silva et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

shot^unspecified/+ ; Eb1⁰⁴⁵²⁴/+ double heterozygous embryonic neurons in primary culture show shortening of axons and increase disorganisation of the microtubules compared to either single heterozygote.

(Alves-Silva et al., 2012)

Dominantly suppresses the S phase defects seen in the CycE^JP eye disc.

(Brumby et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Eb1⁰⁴⁵²⁴ is rescued by Scer\GAL4^unspecified/Eb1^UAS.cKa.GFP

(Alves-Silva et al., 2012)

Eb1⁰⁴⁵²⁴ is rescued by Eb1^Ubi-p63E.PE

(Elliott et al., 2005)

Comments

Expression of Eb1^{Scer\UAS.cKa.T:Avic\GFP} under the control of Scer\GAL4^unspecified significantly rescues the axon extension and microtubule organisation defects of Eb1⁰⁴⁵²⁴/Eb1⁰⁴⁵²⁴ embryonic neurons in primary culture.

(Alves-Silva et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11379

P{PZ}Eb1⁰⁴⁵²⁴ Mccc2⁰⁴⁵²⁴ cn¹/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Comments

After six generations of outcrossing the lethality continued to segragate with the P{PZ} insertion.

(Beaton, 2000.1.31)

Transcription of the Eb1 gene is disrupted in Eb1⁰⁴⁵²⁴ mutants.

(Brumby et al., 2004)

Lethality is reverted at a high frequency by remobilization of the P-element.

(Elliott et al., 2005)

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AF006645

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

DmEB1^P

(Elliott et al., 2005)

EB1⁰⁴⁵²⁴

(Hildebrand et al., 2021)

Eb1⁰⁴⁵²⁴

(Hahn et al., 2021, Christensen and Cook, 2007.3.22)

eb1⁰⁴⁵²⁴

(Baskar et al., 2019, Alves-Silva et al., 2012)

l(2)04524

(Brumby et al., 2004, Spradling et al., 1999)

l(2)04524⁰⁴⁵²⁴

(BDGP Project Members, 1994-1999)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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