FlyBase Allele Report: Dmel\ena[46]

General Information

Symbol

Dmel\ena⁴⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0102726

Feature type

allele

Associated gene

Dmel\ena

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Li et al., 2005)

Mutagen

ethyl methanesulfonate

(Gao et al., 1999)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C?T.

(Li et al., 2005)

Amino acid replacement: Q489term.

(Li et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:19,160,840..19,160,840 [+]

Nucleotide change:

C19160840T

Reported nucleotide change:

C?T

Amino acid change:

Reported amino acid change:

Q489term

(Li et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage

(Li et al., 2005)

abnormal neuroanatomy | somatic clone | third instar larval stage

(Li et al., 2005)

abnormal neuroanatomy | third instar larval stage

(Li et al., 2005)

Phenotype Manifest In

abdominal posterior ventral multidendritic neuron vdap

(Li et al., 2005)

actin filament & egg chamber | germ-line clone

(Gates et al., 2009)

dendrite

(Li et al., 2005)

dendrite | dorsal | embryonic stage

(Gao et al., 1999)

dendrite | somatic clone

(Li et al., 2005)

dendritic arborizing neuron

(Li et al., 2005)

dendritic tree

(Li et al., 2005)

egg | germline clone | maternal effect

(Gates et al., 2009)

larval dorsal multidendritic neuron ddaB | somatic clone

(Li et al., 2005)

larval dorsal multidendritic neuron ddaC | somatic clone

(Li et al., 2005)

larval dorsal multidendritic neuron ddaE | somatic clone

(Li et al., 2005)

larval dorsal multidendritic neuron ddaF | somatic clone

(Li et al., 2005)

Detailed Description

Statement

Reference

22% of eggs derived from females carrying homozygous germline clones show a "dumpless" phenotype.

Egg chambers in females with homozygous germline clones have defects in the cytoplasmic filaments (the bundled cytoplasmic actin filaments extending from the cortex to nuclei). No defects in ring canal formation or growth are seen in mutant egg chambers.

(Gates et al., 2009)

Dorsal cluster dendritic arborisation neurons show dendritic defects in mutant embryos. At 16 hours after egg laying (AEL), extension of the dorsal dendrites toward the dorsal midline appears more or less normal, but there are significantly fewer dynamic transient lateral fine branches compared to controls (suggesting a defect in the initiation of new branches). At 18-20 hours AEL, the number of lateral dendrites that extend toward or reach the adjacent segment boundaries is reduced compared to wild type.

Third larval instar single cell homozygous ddaB, ddaC, ddaE and ddaF neuron clones show a significant decrease in the number of dendritic ends compared to control clones.

Third larval instar ddaE neurons show a significant decrease in the amount of quarternary, tertiary and secondary dendritic branching compared to controls, while primary branching is not significantly affected.

Heterozygous third instar larvae show a slight decrease in the number of dendritic ends extending from the main dorsal dendrites of the vpda neurons compared to wild type.

(Li et al., 2005)

Exhibits misrouting in the dorsal cluster of embryonic dendrites. The early budding and extension of lateral branches appear normal; however at a late stage, some lateral branches turn dorsally instead of extending toward segment boundaries, resulting in an apparent reduction of the number of lateral branches. About 60% of embryos exhibit this phenotype. Mutants show no gross defects in anterior-posterior or dorsal-ventral patterning. The development of epidermis and muscle patterning also appears to be normal.

(Gao et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

ena⁴⁶ has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by p120ctn³⁰⁸

(Li et al., 2005)

Suppressor of

Statement

Reference

ena[+]/ena⁴⁶ is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

ena[+]/ena⁴⁶ is a suppressor | partially of decreased size | adult stage phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

ena[+]/ena⁴⁶ is a suppressor | partially of decreased size | adult stage phenotype of Scer\GAL4^lz-gal4, Shrm^A.UAS

(Hildebrand et al., 2021)

ena[+]/ena⁴⁶ is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^lz-gal4, Shrm^A.UAS

(Hildebrand et al., 2021)

Phenotype Manifest In

Enhanced by

Statement

Reference

ena⁴⁶ has abdominal posterior ventral multidendritic neuron vdap phenotype, enhanceable by p120ctn³⁰⁸

(Li et al., 2005)

Suppressor of

Statement

Reference

ena[+]/ena⁴⁶ is a suppressor | partially of wing phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

ena[+]/ena⁴⁶ is a suppressor | partially of wing disc ventral compartment phenotype of Scer\GAL4^A9, Shrm^A.UAS

(Hildebrand et al., 2021)

ena[+]/ena⁴⁶ is a suppressor | partially of eye phenotype of Scer\GAL4^lz-gal4, Shrm^A.UAS

(Hildebrand et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

p120ctn³⁰⁸ enhances the decrease in the number of dendritic ends extending from the main dorsal dendrites of the vpda neurons which is seen in ena⁴⁶/+ third instar larvae.

(Li et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

ena²³

(Gao et al., 1999)

ena⁰²⁰²⁹

(Gao et al., 1999)

ena²¹⁰

(Gao et al., 1999)