FlyBase Allele Report: Dmel\ena[GC5]

General Information

Symbol

Dmel\ena^GC5

Species

D. melanogaster

Name

FlyBase ID

FBal0043043

Feature type

allele

Associated gene

Dmel\ena

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

gamma ray

Nature of the Allele

Allele class

Mutagen

gamma ray

(Gertler et al., 1995)

Progenitor genotype

Caused by aberration

In(2R)ena^GC5

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon

(Gertler et al., 1995)

embryonic/first instar larval cuticle (with ena^GC1)

(Gates et al., 2007)

embryonic/larval aorta | embryonic stage

(King et al., 2021)

embryonic/larval heart | embryonic stage

(King et al., 2021)

embryonic cardioblast

(King et al., 2021)

embryonic dorsal epidermis (with ena^GC1)

(Gates et al., 2007)

larval intersegmental nerve

(Crowner et al., 2003, Wills et al., 1999)

larval intersegmental nerve branch ISNb of A1-7 (with ena^GC1)

(Song et al., 2010)

larval longitudinal connective

(Gertler et al., 1995)

larval longitudinal connective (with ena^GC1)

(Fan et al., 2003)

peripheral nervous system

(Gertler et al., 1995)

presumptive embryonic/larval central nervous system (with ena^GC1)

(Wills et al., 2002)

presumptive embryonic/larval central nervous system (with ena^GC8)

(Wills et al., 2002)

RP neuron

(Wills et al., 1999)

symmetrical commissure

(Gertler et al., 1995)

Detailed Description

Statement

Reference

ena^GC5 homozygous embryos show holes between contralateral cardioblasts in the heart region of the dorsal vessel, as well as severe cardioblast morphology defects in the aorta and heart (misshapen, appearing stretched and pinched, rather than cuboidal); cardioblasts show diminished adhesion domains; the heart lumen appears larger.

(King et al., 2021)

The ISNb motor axon shows a bypass phenotype (the ISNb axons fail to enter the ventral longitudinal muscle field and instead bypass along the ISN root) in 99% of hemisegments in ena^GC1/ena^GC5 embryos.

(Song et al., 2010)

75% of zygotic ena^GC5/ena^GC1 mutant embryos have a wild-type cuticle, while 21% show puckering along the dorsal midline.

(Gates et al., 2007)

ena^GC5 animals exhibit a weak ISNb bypass phenotype, seen in about 7% of hemisegments.

(Crowner et al., 2003)

ena^GC1/ena^GC5 mutants exhibit approximately 2 defects in longitudinal axon guidance per animal. An average of 18% of segments show defects. No embryos exhibit defects in pCC/MP1.

(Fan et al., 2003)

ena^GC5/ena^GC8 and ena^GC5/ena^GC1 embryos show modest levels of midline crossing errors by axons in the central nervous system.

(Wills et al., 2002)

86% of ISNb axons show a bypass phenotype in ena^GC1/ena^GC5 embryos. This phenotype is partially suppressed by ena^Scer\UAS.cCa expressed under the control of Scer\GAL4^neu. 29% of ISNb axons show a bypass phenotype in ena^GC5/ena^GC8 embryos. A small number of RP neurons follow an aberrant path within the central nervous system.

(Wills et al., 1999)

Lethal in combination with ena^GC1.

(Ahern-Djamali et al., 1998)

Heterozygotes with ena^GC8 have diffuse and loosely bundled longitudinal and commissural axon tracts and some also exhibit axon misguiding. Some homozygous embryos exhibit thinning of longitudinal connectives, increased number of axons exciting the CNS from the longitudinal axons or failure of commissural axons to separate into anterior and posterior axon bundles. The overall organisation of the PNS is disrupted, spacing and organisation of the neurons is irregular and some clusters of neurons are mislocalised.

(Gertler et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ena^GC5/ena^GC1 has lethal phenotype, suppressible | partially by Hsap\VASP^UAS.cADa/Scer\GAL4^e22c

(Ahern-Djamali et al., 1998)

NOT suppressed by

Statement

Reference

ena^GC5/ena^GC1 has abnormal neuroanatomy | embryonic stage phenotype, non-suppressible by Dab¹/Dab[+]

(Song et al., 2010)

Enhancer of

Statement

Reference

ena[+]/ena^GC5 is an enhancer of abnormal neuroanatomy phenotype of DAAM^Ex68

(Matusek et al., 2008)

Suppressor of

Statement

Reference

ena[+]/ena^GC5 is a suppressor | partially of abnormal neuroanatomy | maternal effect | embryonic stage phenotype of Dab²/Dab¹

(Song et al., 2010)

NOT Suppressor of

Statement

Reference

ena[+]/ena^GC5 is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of Abl⁴/Abl¹

(Lin et al., 2009)

Other

Statement

Reference

Df(2R)en-SFX31, ena^GC5 has abnormal neuroanatomy phenotype

(Joly et al., 2007)

Hsap\VASP^UAS.cADa, Scer\GAL4^e22c, ena^GC5/ena^GC1 has viable phenotype

(Ahern-Djamali et al., 1998)

Phenotype Manifest In

Enhanced by

Statement

Reference

ena^GC5 has larval intersegmental nerve phenotype, enhanceable by N^l1N-ts1

(Crowner et al., 2003)

NOT suppressed by

Statement

Reference

ena^GC5/ena^GC1 has larval intersegmental nerve branch ISNb of A1-7 phenotype, non-suppressible by Dab¹/Dab[+]

(Song et al., 2010)

Enhancer of

Statement

Reference

ena[+]/ena^GC5 is an enhancer of neuropil phenotype of DAAM^Ex68

(Matusek et al., 2008)

ena[+]/ena^GC5 is an enhancer of fascicle phenotype of DAAM^Ex68

(Matusek et al., 2008)

ena[+]/ena^GC5 is an enhancer of connective phenotype of DAAM^Ex68

(Matusek et al., 2008)

ena[+]/ena^GC5 is an enhancer of lateral longitudinal fascicle phenotype of DAAM^Ex68

(Matusek et al., 2008)

ena[+]/ena^GC5 is an enhancer of embryo phenotype of DAAM^Ex68

(Matusek et al., 2008)

ena^GC5 is an enhancer of larval intersegmental nerve | heat sensitive phenotype of N^l1N-ts1

(Crowner et al., 2003)

robo[+], ena[+], ena^GC5, robo1⁵ is an enhancer of larval longitudinal connective phenotype of sli¹

(Bashaw et al., 2000)

ena[+]/ena^GC5 is an enhancer of larval longitudinal connective phenotype of robo1⁵, sli[+]/sli¹

(Bashaw et al., 2000)

Suppressor of

Statement

Reference

ena[+]/ena^GC5 is a suppressor | partially of larval intersegmental nerve branch ISNb of A1-7 | maternal effect phenotype of Dab²/Dab¹

(Song et al., 2010)

NOT Suppressor of

Statement

Reference

ena[+]/ena^GC5 is a non-suppressor of NMJ bouton | increased number phenotype of Abl⁴/Abl¹

(Lin et al., 2009)

Other

Statement

Reference

Df(2R)en-SFX31, ena^GC5 has larval ventral nerve cord phenotype

(Joly et al., 2007)

Df(2R)en-SFX31, ena^GC5 has larval anterior commissure phenotype

(Joly et al., 2007)

Df(2R)en-SFX31, ena^GC5 has larval posterior commissure phenotype

(Joly et al., 2007)

Df(2R)en-SFX31, ena^GC5 has lateral tract phenotype

(Joly et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The penetrance of the ISNb stall phenotype seen in embryos lacking both maternal and zygotic Dab function (derived from Dab¹/Dab² females and having Dab¹ as the paternally derived copy of Dab) is decreased by ena^GC5/+ to 26%.

The severe ISNb bypass phenotype seen in ena^GC1/ena^GC5 embryos is not suppressed by Dab¹/+.

(Song et al., 2010)

The increased bouton number per muscle area that is seen at the neuromuscular junction of Abl¹/Abl⁴ larvae is not suppressed by ena^GC5/+.

The increased bouton number per muscle area that is seen at the neuromuscular junction of Abl¹/Abl⁴ larvae is significantly suppressed by Abi^KO/+. This suppression further increased if the flies are also carrying ena^GC5/+.

(Lin et al., 2009)

An ena^GC5 heterozygous background increases the proportion of DAAM^Ex68 mutant embryos that exhibit CNS defects.

(Matusek et al., 2008)

Stage 15 transheterozygous Df(2R)en-SFX31/ena^GC5 embryos exhibit defects in central nervous system scaffolding with gaps in longitudinal tracts, loss of posterior commissures, or fusion of commissures in about 74.5% of the segments.

Stage 15 transheterozygous en^E/ena^GC5 embryos exhibit defects in central nervous system scaffolding with gaps in longitudinal tracts, loss of posterior commissures, or fusion of commissures in about 15.2% of the segments.

(Joly et al., 2007)

When ena^GC5/ena^GC1 and homozygous dock³ are combined only a mild additive effect is see on the longitudinal exon guidance phenotype.

(Fan et al., 2003)

Xenogenetic Interactions

Statement

Reference

Hsap\VASP^{Scer\UAS.cADa} partially rescues the lethality of ena^GC1/ena^GC5 flies when expressed under the control of Scer\GAL4^e22c; 25-85% of flies are rescued depending on the Hsap\VASP^{Scer\UAS.cADa} line used.

(Ahern-Djamali et al., 1998)

Complementation and Rescue Data

Fails to complement

ena^C14-06

(Sano et al., 2012)

ena⁴⁶

(Gao et al., 1999)

Partially rescued by

ena^GC5 is partially rescued by ena^UAS.GFP/Scer\GAL4^how-24B

(King et al., 2021)

ena^GC5/ena^GC1 is partially rescued by Scer\GAL4^neu/ena^{UAS.Tag:polyHis}

(Wills et al., 1999)

ena^GC5/ena^GC1 is partially rescued by Scer\GAL4^e22c/ena^UAS.cADa

(Ahern-Djamali et al., 1998)

ena^GC5/ena^GC1 is partially rescued by ena^K636stop.UAS/Scer\GAL4^e22c

(Ahern-Djamali et al., 1998)

ena^GC5/ena^GC1 is partially rescued by Scer\GAL4^e22c/ena^{UAS.Tag:polyHis}

(Comer et al., 1998)

ena^GC5/ena^GC1 is partially rescued by Scer\GAL4^e22c/ena^{YF.UAS.Tag:polyHis}

(Comer et al., 1998)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8570

Adh^fn6 pr¹ cn¹ ena^GC5/CyO, P{ftz-lacB}E3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ena^GC5

(King et al., 2021, Sano et al., 2012, Gonçalves-Pimentel et al., 2011, Song et al., 2010, Lin et al., 2009, Matusek et al., 2008, Gates et al., 2007, Joly et al., 2007)

enb^GC5

Name Synonyms

Secondary FlyBase IDs

References (20)

Report Sections

Open Close