Imprecise excision of the progenitor insertion, resulting in a 3070bp deletion which includes the 5' promoter region and the first exon (which includes the translation start site) of Dab.
3070bp deletion which begins 1980bp upstream of the Dab start codon and ends 1090bp downstream of the start codon.
Imprecise excision of the progenitor insertion, resulting in a 3070bp deletion.
embryo | dorsal closure stage | maternal effect (with Dab2)
embryo | embryonic stage 5 | maternal effect (with Dab2)
ISNb motor axons stall and fail to innervate their most distal target (muscle 12) in 46% of hemisegments in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab1 as the paternally derived copy of Dab). These embryos show a "stop short" phenotype in the dorsal branch of the SNa motor nerve, with the axons stopping short and failing to reach their muscle target in 39% of hemisegments.
ISNb motor axons stall and fail to innervate their most distal target (muscle 12) in 59% of hemisegments in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab2 as the paternally derived copy of Dab). These embryos show a "stop short" phenotype in the dorsal branch of the SNa motor nerve, with the axons stopping short and failing to reach their muscle target in 33% of hemisegments.
ISNb motor axons stall and fail to innervate their most distal target (muscle 12) in 9% of hemisegments in homozygous embryos. These embryos show a "stop short" phenotype in the dorsal branch of the SNa motor nerve, with the axons stopping short and failing to reach their muscle target in 9% of hemisegments.
Embryos lacking both maternal and zygotic Dab function (derived from a Dab1/Dab2 stock) show defects during cellularisation; anucleated pseudocells, incomplete pseudocleavage furrows and multinucleated cells are seen during cellularisation, with 92% of embryos having defective cells. The formation of the peri-nuclear tubulin baskets appears to be normal. Abnormal apical actin accumulation is seen throughout cellularisation in these embryos and variable lateral actin accumulation is also seen.
Embryos lacking both maternal and zygotic Dab function (derived from a Dab1/Dab2 stock) show defects in dorsal closure with occasional breaks of the leading edge, failure of cell elongation and disturbances in the "zippering" of the dorsal epithelia.
Dab1 has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by In(3L)std11/+
Dab1 has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by trio1/trio[+]
Dab1 has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by Df(3L)st100.62/+
Dab2/Dab1 has abnormal neuroanatomy | embryonic stage | maternal effect phenotype, enhanceable by trio8/trio[+]
Dab2/Dab1 has abnormal neuroanatomy | embryonic stage | maternal effect phenotype, enhanceable by In(3L)std11/+
Dab2/Dab1 has abnormal neuroanatomy | embryonic stage | maternal effect phenotype, enhanceable by Df(3L)st100.62/+
Dab2/Dab1 has abnormal neuroanatomy | embryonic stage | maternal effect phenotype, enhanceable by trio1/trio[+]
Dab2/Dab1 has abnormal neuroanatomy | maternal effect | embryonic stage phenotype, suppressible | partially by ena[+]/enaGC5
Dab2/Dab1 has abnormal neuroanatomy | maternal effect | embryonic stage phenotype, suppressible | partially by ena[+]/enaGC1
Dab2/Dab1 has abnormal neuroanatomy | maternal effect | embryonic stage phenotype, suppressible | partially by Scer\GAL4elav.PU/AblUAS.cFa
Dab1/Dab[+] is a non-suppressor of abnormal neuroanatomy | embryonic stage phenotype of enaGC5/enaGC1
Dab2/Dab1 has larval intersegmental nerve branch ISNb of A1-7 | maternal effect phenotype, enhanceable by In(3L)std11/+
Dab2/Dab1 has larval segmental nerve branch SNa of A1-7 | maternal effect phenotype, enhanceable by In(3L)std11/+
Dab1 has larval segmental nerve branch SNa of A1-7 phenotype, enhanceable by In(3L)std11/+
Dab1 has larval intersegmental nerve branch ISNb of A1-7 phenotype, enhanceable by trio1/trio[+]
Dab1 has larval segmental nerve branch SNa of A1-7 phenotype, enhanceable by trio1/trio[+]
Dab1 has larval intersegmental nerve branch ISNb of A1-7 phenotype, enhanceable by Df(3L)st100.62/+
Dab2/Dab1 has larval intersegmental nerve branch ISNb of A1-7 | maternal effect phenotype, enhanceable by trio8/trio[+]
Dab2/Dab1 has larval intersegmental nerve branch ISNb of A1-7 | maternal effect phenotype, enhanceable by Df(3L)st100.62/+
Dab2/Dab1 has larval segmental nerve branch SNa of A1-7 | maternal effect phenotype, enhanceable by Df(3L)st100.62/+
Dab2/Dab1 has larval intersegmental nerve branch ISNb of A1-7 | maternal effect phenotype, enhanceable by trio1/trio[+]
Dab2/Dab1 has larval segmental nerve branch SNa of A1-7 | maternal effect phenotype, enhanceable by trio1/trio[+]
Dab1 has larval intersegmental nerve branch ISNb of A1-7 phenotype, enhanceable by In(3L)std11/+
Dab2/Dab1 has larval intersegmental nerve branch ISNb of A1-7 | maternal effect phenotype, suppressible | partially by ena[+]/enaGC5
Dab2/Dab1 has larval intersegmental nerve branch ISNb of A1-7 | maternal effect phenotype, suppressible | partially by ena[+]/enaGC1
Dab2/Dab1 has larval intersegmental nerve branch ISNb of A1-7 | maternal effect phenotype, suppressible | partially by Scer\GAL4elav.PU/AblUAS.cFa
Dab1/Dab[+] is a non-suppressor of larval intersegmental nerve branch ISNb of A1-7 phenotype of enaGC5/enaGC1
The penetrance of the ISNb stall and SNa dorsal branch stop short phenotypes seen in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab1 as the paternally derived copy of Dab) is increased by In(3L)std11/+ to 77% and 69% respectively.
The penetrance of the ISNb stall and SNa dorsal branch stop short phenotypes seen in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab1 as the paternally derived copy of Dab) is increased by Df(3L)st100.62/+ to 65% and 57% respectively.
The penetrance of the ISNb stall and SNa dorsal branch stop short phenotypes seen in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab1 as the paternally derived copy of Dab) is increased by trio1/+ to 78% and 80% respectively.
The penetrance of the ISNb stall phenotype seen in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab1 as the paternally derived copy of Dab) is increased by trio8/+ to 80%.
The penetrance of the ISNb stall and SNa dorsal branch stop short phenotypes seen in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab2 as the paternally derived copy of Dab) is increased by In(3L)std11/+ to 78% and 62% respectively.
The penetrance of the ISNb stall and SNa dorsal branch stop short phenotypes seen Dab1 homozygous embryos is increased by In(3L)std11/+ to 59% and 52% respectively.
The penetrance of the ISNb stall and SNa dorsal branch stop short phenotypes seen Dab1 homozygous embryos is increased by trio1/+ to 72% and 35% respectively.
The penetrance of the ISNb stall phenotype seen Dab1 homozygous embryos is increased by Df(3L)st100.62/+ to 54%.
The penetrance of the ISNb stall phenotype seen in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab1 as the paternally derived copy of Dab) is decreased by enaGC5/+ to 26%.
The penetrance of the ISNb stall phenotype seen in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab1 as the paternally derived copy of Dab) is decreased by enaGC1/+ to 36%.
The penetrance of the ISNb stall phenotype seen in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab1 as the paternally derived copy of Dab) is decreased by expression of AblScer\UAS.cFa under the control of Scer\GAL4elav.PU to 29%.
The severe ISNb bypass phenotype seen in enaGC1/enaGC5 embryos is not suppressed by Dab1/+.
Dab2/Dab1 is partially rescued by DabUAS.cWa/Scer\GAL4elav.PU
Dab2/Dab1 is partially rescued by DabUAS.cWa/Scer\GAL4sca-537.4
Expression of DabScer\UAS.cWa under the control of Scer\GAL4elav.PU partially rescues the ISNb stall phenotype seen in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab1 as the paternally derived copy of Dab).
Expression of DabScer\UAS.cWa under the control of Scer\GAL4sca-537.4 partially rescues the ISNb stall phenotype seen in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab2 as the paternally derived copy of Dab).