Imprecise excision of the progenitor insertion, resulting in a 1887bp deletion which includes the 5' promoter region and the first exon (which includes the translation start site) of Dab.
1887bp deletion which begins 1695bp upstream of the Dab start codon and ends 192bp downstream of the start codon.
1887bp deletion resulting from the imprecise excision of P{EPgy2}DabEY10190 which begins 1695bp upstream of the Dab start codon and ends 192bp downstream of the start codon.
embryo | dorsal closure stage | maternal effect (with Dab1)
embryo | embryonic stage 5 | maternal effect (with Dab1)
ISNb motor axons stall and fail to innervate their most distal target (muscle 12) in 46% of hemisegments in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab1 as the paternally derived copy of Dab). These embryos show a "stop short" phenotype in the dorsal branch of the SNa motor nerve, with the axons stopping short and failing to reach their muscle target in 39% of hemisegments.
ISNb motor axons stall and fail to innervate their most distal target (muscle 12) in 59% of hemisegments in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab2 as the paternally derived copy of Dab). These embryos show a "stop short" phenotype in the dorsal branch of the SNa motor nerve, with the axons stopping short and failing to reach their muscle target in 33% of hemisegments.
Embryos lacking both maternal and zygotic Dab function (derived from a Dab1/Dab2 stock) show defects during cellularisation; anucleated pseudocells, incomplete pseudocleavage furrows and multinucleated cells are seen during cellularisation, with 92% of embryos having defective cells. The formation of the peri-nuclear tubulin baskets appears to be normal. Abnormal apical actin accumulation is seen throughout cellularisation in these embryos and variable lateral actin accumulation is also seen.
Embryos lacking both maternal and zygotic Dab function (derived from a Dab1/Dab2 stock) show defects in dorsal closure with occasional breaks of the leading edge, failure of cell elongation and disturbances in the "zippering" of the dorsal epithelia.
Adult escapers are occasionally seen.
Dab2/Dab1 has abnormal neuroanatomy | embryonic stage | maternal effect phenotype, enhanceable by trio8/trio[+]
Dab2/Dab1 has abnormal neuroanatomy | embryonic stage | maternal effect phenotype, enhanceable by In(3L)std11/+
Dab2/Dab1 has abnormal neuroanatomy | embryonic stage | maternal effect phenotype, enhanceable by Df(3L)st100.62/+
Dab2/Dab1 has abnormal neuroanatomy | embryonic stage | maternal effect phenotype, enhanceable by trio1/trio[+]
Dab2/Dab1 has abnormal neuroanatomy | maternal effect | embryonic stage phenotype, suppressible | partially by ena[+]/enaGC5
Dab2/Dab1 has abnormal neuroanatomy | maternal effect | embryonic stage phenotype, suppressible | partially by ena[+]/enaGC1
Dab2/Dab1 has abnormal neuroanatomy | maternal effect | embryonic stage phenotype, suppressible | partially by Scer\GAL4elav.PU/AblUAS.cFa
Dab2/Dab1 has larval intersegmental nerve branch ISNb of A1-7 | maternal effect phenotype, enhanceable by In(3L)std11/+
Dab2/Dab1 has larval segmental nerve branch SNa of A1-7 | maternal effect phenotype, enhanceable by In(3L)std11/+
Dab2/Dab1 has larval intersegmental nerve branch ISNb of A1-7 | maternal effect phenotype, enhanceable by Df(3L)st100.62/+
Dab2/Dab1 has larval segmental nerve branch SNa of A1-7 | maternal effect phenotype, enhanceable by Df(3L)st100.62/+
Dab2/Dab1 has larval intersegmental nerve branch ISNb of A1-7 | maternal effect phenotype, enhanceable by trio1/trio[+]
Dab2/Dab1 has larval segmental nerve branch SNa of A1-7 | maternal effect phenotype, enhanceable by trio1/trio[+]
Dab2/Dab1 has larval intersegmental nerve branch ISNb of A1-7 | maternal effect phenotype, enhanceable by trio8/trio[+]
Dab2/Dab1 has larval intersegmental nerve branch ISNb of A1-7 | maternal effect phenotype, suppressible | partially by ena[+]/enaGC5
Dab2/Dab1 has larval intersegmental nerve branch ISNb of A1-7 | maternal effect phenotype, suppressible | partially by ena[+]/enaGC1
Dab2/Dab1 has larval intersegmental nerve branch ISNb of A1-7 | maternal effect phenotype, suppressible | partially by Scer\GAL4elav.PU/AblUAS.cFa
The penetrance of the ISNb stall and SNa dorsal branch stop short phenotypes seen in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab1 as the paternally derived copy of Dab) is increased by In(3L)std11/+ to 77% and 69% respectively.
The penetrance of the ISNb stall and SNa dorsal branch stop short phenotypes seen in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab1 as the paternally derived copy of Dab) is increased by Df(3L)st100.62/+ to 65% and 57% respectively.
The penetrance of the ISNb stall and SNa dorsal branch stop short phenotypes seen in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab1 as the paternally derived copy of Dab) is increased by trio1/+ to 78% and 80% respectively.
The penetrance of the ISNb stall phenotype seen in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab1 as the paternally derived copy of Dab) is increased by trio8/+ to 80%.
The penetrance of the ISNb stall and SNa dorsal branch stop short phenotypes seen in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab2 as the paternally derived copy of Dab) is increased by In(3L)std11/+ to 78% and 62% respectively.
The penetrance of the ISNb stall phenotype seen in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab1 as the paternally derived copy of Dab) is decreased by enaGC5/+ to 26%.
The penetrance of the ISNb stall phenotype seen in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab1 as the paternally derived copy of Dab) is decreased by enaGC1/+ to 36%.
The penetrance of the ISNb stall phenotype seen in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab1 as the paternally derived copy of Dab) is decreased by expression of AblScer\UAS.cFa under the control of Scer\GAL4elav.PU to 29%.
Dab2/Dab1 is partially rescued by DabUAS.cWa/Scer\GAL4elav.PU
Dab2/Dab1 is partially rescued by DabUAS.cWa/Scer\GAL4sca-537.4
Expression of DabScer\UAS.cWa under the control of Scer\GAL4elav.PU partially rescues the ISNb stall phenotype seen in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab1 as the paternally derived copy of Dab).
Expression of DabScer\UAS.cWa under the control of Scer\GAL4sca-537.4 partially rescues the ISNb stall phenotype seen in embryos lacking both maternal and zygotic Dab function (derived from Dab1/Dab2 females and having Dab2 as the paternally derived copy of Dab).
