FlyBase Allele Report: Dmel\trio[8]

General Information

Symbol

Dmel\trio⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0117302

Feature type

allele

Associated gene

Dmel\trio

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Newsome et al., 2000)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q613term.

(Newsome et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:1,027,398..1,027,398 [-]

Nucleotide change:

C1027398T

Amino acid change:

Q613term | trio-PA; Q613term | trio-PC

Reported amino acid change:

Q613term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Newsome et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage (with trio¹)

(Song et al., 2010)

abnormal neuroanatomy | somatic clone | third instar larval stage

(Berger et al., 2008)

lethal | recessive

(Newsome et al., 2000)

Phenotype Manifest In

axon | somatic clone | third instar larval stage

(Berger et al., 2008)

embryonic somatic muscle cell (with trio¹)

(Hakeda-Suzuki et al., 2002)

larval intersegmental nerve branch ISNb of A1-7 (with trio¹)

(Song et al., 2010)

larval intersegmental nerve branch ISNb of A1-7 | embryonic stage (with trio¹)

(Kannan et al., 2017)

larval intersegmental nerve branch ISNb of A1-7 | embryonic stage (with trio^123.4)

(Kannan et al., 2017)

larval segmental nerve branch SNa of A1-7 (with trio¹)

(Song et al., 2010)

photoreceptor cell & axon (with trio^S138606)

(Newsome et al., 2000)

photoreceptor cell & axon | somatic clone

(Newsome et al., 2000)

photoreceptor cell R7 & axon | somatic clone

(Newsome et al., 2000)

Detailed Description

Statement

Reference

trio⁸/trio^123.4 or trio⁸/trio¹ embryos display stalling of the ISNb motor nerve at the junction of muscles 6 and 13 with failure to innervate muscle 12 in >65% of hemisegments examined.

(Kannan et al., 2017)

trio¹/trio⁸ embryos show defects in motor axon patterning; ISNb motor axons stall and fail to innervate their most distal target and the embryos show a "stop short" phenotype in the dorsal branch of the SNa motor nerve, with the axons stopping short and failing to reach their muscle target.

(Song et al., 2010)

Photoreceptor axons do not project correctly in the optic lobe of trio⁸ eye-brain complexes in third instar larvae, while the photoreceptor cells themselves are appropriately specified.

A portion of the axons growing from the dorsal and ventral regions of the eye disc stall within the optic stalk or enter the optic lobe but fail to reach their normal target region in trio⁸ whole-eye Scer\FLP1^ey.PN clones in third instar larvae.

(Berger et al., 2008)

Dorsal closure occurs normally in trio¹/trio⁸ embryos derived from trio¹/trio¹ mothers. Myoblast fusion appears complete, but myotubes often fail to attach correctly to the epidermis.

(Hakeda-Suzuki et al., 2002)

Defects in pathfinding by photoreceptor axons are seen in mosaic animals in which virtually the entire retina is homozygous for trio⁸ while other tissues are heterozygous (clones generated using the "eyFLP" system); a striking misrouting of axon bundles beyond the medulla and into deeper regions of the brain is seen in 61% of hemispheres of mosaic adults. In most cases, these misrouted axons pass around the posterior edge of the medulla and then turn anteriorly to run between the medulla and the underlying lobula. In mosaic larvae, almost all R1-R6 axons terminate correctly in the lamina. R7 axons which enter the medulla also generally recognise this ganglion as their appropriate target layer, although they terminate in highly disordered arrays. However, many R7 axons are completely misrouted around the posterior edge of the medulla and contribute to the axon bundles extending between the medulla and underlying lobula. The axons of the polar (dorsal- and ventral-most) photoreceptors retain their normal topographic order in the optic stalk and then fan out correctly to reach the dorsal and ventral extremes of the optic stalk lobes. However, 18% of these polar axon bundles appear to stall within the optic stalk. Less than 10% of trio^S138606/trio¹ animals show defects in photoreceptor axon projection, having a "medulla bypass" phenotype.

(Newsome et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

trio⁸/trio[+] is an enhancer of abnormal neuroanatomy | embryonic stage | maternal effect phenotype of Dab²/Dab¹

(Song et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

trio^S138606/trio⁸ has phenotype, enhanceable by Pak[+]/Pak¹⁶

(Newsome et al., 2000)

trio^S138606/trio⁸ has photoreceptor cell & axon phenotype, enhanceable by Pak[+]/Pak¹⁶

(Newsome et al., 2000)

trio^S138606/trio⁸ has phenotype, enhanceable by Pak[+]/Pak²⁰

(Newsome et al., 2000)

trio^S138606/trio⁸ has photoreceptor cell & axon phenotype, enhanceable by Pak[+]/Pak²⁰

(Newsome et al., 2000)

NOT suppressed by

Statement

Reference

trio⁸/trio^123.4 has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype, non-suppressible by Scer\GAL4^elav.PU/Abl^UAS.cFa

(Kannan et al., 2017)

Enhancer of

Statement

Reference

trio⁸/trio[+] is an enhancer of larval intersegmental nerve branch ISNb of A1-7 | maternal effect phenotype of Dab²/Dab¹

(Song et al., 2010)

trio⁸/trio[+] is an enhancer of phenotype of dock⁴/dock⁰⁴⁷²³

(Newsome et al., 2000)

trio⁸/trio[+] is an enhancer of photoreceptor cell & axon phenotype of dock⁴/dock⁰⁴⁷²³

(Newsome et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The stalling of the ISNb motor nerve at the junction of muscles 6 and 13 with failure to innervate muscle 12 characteristic for trio⁸/trio^123.4 mutant embryos is not suppressed by Scer\GAL4^elav.PU-driven expression of Abl^Scer\UAS.cFa in the mutant background.

(Kannan et al., 2017)

The penetrance of the ISNb stall phenotype seen in embryos lacking both maternal and zygotic Dab function (derived from Dab¹/Dab² females and having Dab¹ as the paternally derived copy of Dab) is increased by trio⁸/+ to 80%.

(Song et al., 2010)

More than 80% of dock⁴/dock⁰⁴⁷²³ animals which are also heterozygous for trio⁴ show defects in photoreceptor axon projection, having a "medulla bypass" phenotype. The frequency of trio^S138606/trio⁸ animals showing defects in photoreceptor axon projection (having a "medulla bypass" phenotype) is enhanced if they are also heterozygous for either Pak¹⁶ or Pak²⁰.