FlyBase Allele Report: Dmel\DAAM[Ex68]

General Information

Symbol

Dmel\DAAM^Ex68

Species

D. melanogaster

Name

FlyBase ID

FBal0194072

Feature type

allele

Associated gene

Dmel\DAAM

Associated Insertion(s)

Carried in Construct

Also Known As

dDAAM^Ex68

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Matusek et al., 2006)

Mutagen

P-element activity

(Matusek et al., 2006)

Progenitor genotype

P{EP}EP1542

(Matusek et al., 2006)

Cytology

Description

Imprecise excision of the P{EP}EP1542 element, resulting in deletion of the C-terminal 457 amino acids, including sequences corresponding to the 'DAD' domain and most of the 'FH2' domain.

(Matusek et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | late third instar larval stage

(Molnár et al., 2014)

abnormal neuroanatomy

(Matusek et al., 2008)

abnormal neuroanatomy (with DAAM^Ex1)

(Matusek et al., 2008)

abnormal neuroanatomy | larval stage

(Migh et al., 2018)

abnormal neuroanatomy | P-stage (with DAAM^Ex4)

(Gazsó-Gerhát et al., 2023)

abnormal neuroanatomy | somatic clone

(Gombos et al., 2015)

abnormal neurophysiology | larval stage, with DAAM^UAS.cMb, Scer\GAL4^btl.PS

(Migh et al., 2018)

abnormal size | adult stage

(Migh et al., 2018)

decreased body size | larval stage

(Migh et al., 2018)

lethal (with DAAM^Ex1)

(Matusek et al., 2006)

lethal (with DAAM^Ex36)

(Matusek et al., 2006)

lethal (with DAAM^Ex65)

(Matusek et al., 2006)

lethal (with DAAM^Ex184)

(Matusek et al., 2006)

lethal (with DAAM^Ex202)

(Matusek et al., 2006)

lethal (with DAAM^Ex249)

(Matusek et al., 2006)

lethal | recessive

(Matusek et al., 2006)

Phenotype Manifest In

actin filament bundle | embryonic stage 17

(Öztürk-Çolak et al., 2016)

axon | embryonic stage

(Migh et al., 2018)

connective

(Matusek et al., 2008)

connective (with DAAM^Ex1)

(Matusek et al., 2008)

embryo

(Matusek et al., 2008)

embryo (with DAAM^Ex1)

(Matusek et al., 2008)

embryonic/larval cuticle

(Matusek et al., 2006)

embryonic/larval neuromuscular junction | larval stage

(Migh et al., 2018)

embryonic/larval neuromuscular junction | larval stage, with DAAM^UAS.cMb, Scer\GAL4^btl.PS

(Migh et al., 2018)

embryonic/larval somatic muscle cell | late third instar larval stage

(Molnár et al., 2014)

embryonic/larval tracheal dorsal trunk | embryonic stage 17

(Öztürk-Çolak et al., 2016)

embryonic/larval tracheal system

(Matusek et al., 2006)

embryonic/larval tracheal system (with Df(1)AD11)

(Matusek et al., 2006)

embryonic somatic muscle cell

(Migh et al., 2018)

fascicle

(Matusek et al., 2008)

fascicle (with DAAM^Ex1)

(Matusek et al., 2008)

filamentous actin

(Matusek et al., 2006)

filopodium (with DAAM^Ex1)

(Gonçalves-Pimentel et al., 2011)

larval A1-7-innervating LL1 motor neuron | larval stage

(Migh et al., 2018)

larval A1-7-innervating LL1 motor neuron | larval stage, with DAAM^UAS.cMb, Scer\GAL4^btl.PS

(Migh et al., 2018)

larval A1-7-innervating VL3/4 motor neuron | larval stage

(Migh et al., 2018)

larval intersegmental nerve | embryonic stage

(Migh et al., 2018)

lateral longitudinal fascicle

(Matusek et al., 2008)

lateral longitudinal fascicle (with DAAM^Ex1)

(Matusek et al., 2008)

microtubule | larval stage, with DAAM^UAS.cMb, Scer\GAL4^btl.PS

(Migh et al., 2018)

muscle cell of A1-7 ventral longitudinal muscle 3 | late third instar larval stage

(Molnár et al., 2014)

mushroom body | somatic clone

(Gombos et al., 2015)

neuroblast | somatic clone

(Gombos et al., 2015)

neuropil

(Matusek et al., 2008)

neuropil (with DAAM^Ex1)

(Matusek et al., 2008)

NMJ bouton | larval stage

(Migh et al., 2018)

NMJ bouton | larval stage, with DAAM^UAS.cMb, Scer\GAL4^btl.PS

(Migh et al., 2018)

ommatidium | P-stage (with DAAM^Ex4)

(Gazsó-Gerhát et al., 2023)

sarcomere | late third instar larval stage

(Molnár et al., 2014)

synaptic vesicle | larval stage

(Migh et al., 2018)

taenidial fold

(Matusek et al., 2006)

taenidial fold | embryonic stage 17

(Öztürk-Çolak et al., 2016)

Detailed Description

Statement

Reference

DAAM^Ex68 mutant larvae develop small muscles, as compared to controls.

The ISNb nerve terminals in DAAM^Ex68 mutant embryos often show guidance defects and premature terminations, as compared to controls.

The larval neuromuscular junctions at muscles 4 and 6/7 in DAAM^Ex68 hemizygous males are significantly smaller, display significantly fewer synaptic boutons and significantly fewer synaptic vesicles, but do not show actin organization defects nor obvious active zone or T-bar defects, as compared to controls; the enervated muscles, however, do not show significant size defects, as compared to controls. DAAM^Ex68 heterozygous females, however, do not show defects in synaptic bouton number compared to controls.

The larval muscle 4 neuromuscular junctions in DAAM^Ex68 hemizygous males expressing DAAM^UAS.cMb under the control of Scer\GAL4^btl.PS larvae show fragmented/absent presynaptic microtubules bundles and 'bouton fusion', as compared to controls. Synaptic transmission across this junction show a significant decrease in evoked excitatory junction current amplitude, but do not show significant changes in evoked excitatory junction current frequency or in miniature excitatory junction current amplitude, as compared to controls.

(Migh et al., 2018)

The tracheal dorsal trunk of DAAM^Ex68 homozygous stage 17 embryos exhibit heterogeneous patterns of hardly detected taenidial folds and thin F-actin bundles with stretches of perpendicular structures followed by stretches of parallel structures when compared to controls.

(Öztürk-Çolak et al., 2016)

Single cell DAAM^Ex68 mutant mushroom body clones in a DAAM^Ex68/+ mutant background display a wild type morphology with a single bifurcated axon.

DAAM^Ex68 mutant neuroblast clones in a DAAM^Ex68/+ mutant background display abnormal axonal morphology in the mushroom bodies.

(Gombos et al., 2015)

The body size and somatic musculature of early DAAM^Ex68 third instar larvae appear normal, but late third instar larvae (100 hours after egg laying) are shorter than normal and have slight muscle defects (muscles are smaller than normal, some myofibres are split and their general organisation is looser than in wild type). Muscle VL3 is reduced in both length and width, due to both sarcomere shortening and a reduction in sarcomere numbers.

Motility of mutant early third instar larvae is normal, but mutant late third instar larvae have significantly reduced motility compared to wild type.

(Molnár et al., 2014)

Cultured primary neurons derived from DAAM^Ex1/DAAM^Ex68 embryos show a significant reduction in the number of filopodia compared to control neurons.

(Gonçalves-Pimentel et al., 2011)

DAAM^Ex68 homozygous mutant embryos display subtle defects in the neuropile, such as thinning of or gaps in the connectives (in 1.5% of embryos), or partial lack of the lateral most Fas2 positive tract. DAAM^Ex68 maternal and zygotic mutant embryos fail to cellularise properly.

DAAM^Ex68/DAAM^Ex1 mutant embryos (in which both maternal and zygotic functions are impaired) show severe CNS phenotypes. Thirty-four percent of these embryos exhibit severe morphological aberrations, some with completely disorganized nerve cords. Another 35% display frequent breaks in connectives and commissures, misrouted axons, or failures in commissure separation and, in extreme cases, an almost complete lack of axon bundles. There do not appear to be differences in neuron numbers between DAAM^Ex68/DAAM^Ex1 and wild-type embryos, suggesting that the CNS phenotypes are caused by defects in neurite growth rather than aberrant lineage formation. Axonal growth defects are seen before stages 12 and 13, indicating cell death/degeneration is not involved.

Primary neuronal cultures developed from DAAM^Ex68/DAAM^Ex1 or DAAM^Ex68 homozygous mutant embryos are able to develop axons of similar length as their wild-type counterparts. However, filopodia of mutant neurons are reduced in number by 62% and in length by 26%.

(Matusek et al., 2008)

DAAM^Ex68 mutant larvae display severe trachea defects, including the collapse and flattening of the tracheal tubes in both the main airways and the side branches. Tracheal tubes of DAAM^Ex68 mutant larvae fail to secrete such a highly ordered cuticle as that observed in wild-type larvae. Although some local order is visible, short and curvy apical ridges that rarely run perpendicular to the tube axis can be observed throughout the tracheal system.

DAAM^Ex68/Df(1)AD11 transheterozygotes show the same strong larval tracheal phenotype as DAAM^Ex68 homozygotes.

In contrast to the actin organization found in wild-type tracheal cells, in DAAM^Ex68 mutants this organization is completely abolished. The overall level of apical F-actin is not reduced in the DAAM^Ex68 mutant tracheal cells but the actin bundles display abnormal morphology and fail to be organized into parallel running actin rings under the apical surface of the cells. Although the global actin organization is severely impaired , local order can often be detected in small patches within a cell. The cuticle pattern of DAAM^Ex68 mutants displays a similar phenotype.

(Matusek et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

DAAM^Ex4/DAAM^Ex68 has abnormal neuroanatomy | P-stage phenotype, enhanceable by Cdc42²

(Gazsó-Gerhát et al., 2023)

DAAM^Ex68 has abnormal neuroanatomy phenotype, enhanceable by Rac1^J11

(Matusek et al., 2008)

DAAM^Ex68 has abnormal neuroanatomy phenotype, enhanceable by Rac1^J11/Rac2^Δ

(Matusek et al., 2008)

DAAM^Ex68, Rac1^J11 has abnormal neuroanatomy phenotype, enhanceable by Rac2^Δ

(Matusek et al., 2008)

DAAM^Ex68 has abnormal neuroanatomy phenotype, enhanceable by Rac1^J11/Rac2^Δ/Mtl^Δ

(Matusek et al., 2008)

DAAM^Ex68, Rac1^J11, Rac2^Δ has abnormal neuroanatomy phenotype, enhanceable by Mtl^Δ

(Matusek et al., 2008)

DAAM^Ex68 has abnormal neuroanatomy phenotype, enhanceable by chic[+]/chic²²¹

(Matusek et al., 2008)

DAAM^Ex68 has abnormal neuroanatomy phenotype, enhanceable by ena[+]/ena^GC5

(Matusek et al., 2008)

DAAM^Ex68 has abnormal neuroanatomy phenotype, enhanceable by Rho1^72F

(Matusek et al., 2008)

DAAM^Ex68 has abnormal neuroanatomy phenotype, enhanceable by ena⁰²⁰²⁹/ena[+]

(Matusek et al., 2008)

Suppressed by

Statement

Reference

DAAM^Ex1/DAAM^Ex68 has abnormal neuroanatomy phenotype, suppressible | partially by Scer\GAL4^elav-C155/Mmus\Daam1^UAS.EGFP

(Matusek et al., 2008)

Other

Statement

Reference

DAAM^Ex68, Frl^Ex83 has decreased cell size | somatic clone | cell autonomous | adult stage phenotype

(Dollar et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

DAAM^Ex4/DAAM^Ex68 has ommatidium | P-stage phenotype, enhanceable by Cdc42²

(Gazsó-Gerhát et al., 2023)

DAAM^Ex68 has NMJ bouton | larval stage phenotype, enhanceable by Ank2^f00518/Ank2^f00518

(Migh et al., 2018)

DAAM^Ex68 has embryonic/larval neuromuscular junction | larval stage phenotype, enhanceable by futsch^CR13

(Migh et al., 2018)

DAAM^Ex68 has larval LL1 motor neuron | larval stage phenotype, enhanceable by futsch^CR13

(Migh et al., 2018)

DAAM^Ex1/DAAM^Ex68 has filopodium phenotype, enhanceable by Arpc1¹

(Gonçalves-Pimentel et al., 2011)

DAAM^Ex68 has neuropil phenotype, enhanceable by Rho1^72F

(Matusek et al., 2008)

DAAM^Ex68 has fascicle phenotype, enhanceable by Rho1^72F

(Matusek et al., 2008)

DAAM^Ex68 has embryo phenotype, enhanceable by Rac1^J11

(Matusek et al., 2008)

DAAM^Ex68 has neuropil phenotype, enhanceable by Rac1^J11/Rac2^Δ

(Matusek et al., 2008)

DAAM^Ex68 has fascicle phenotype, enhanceable by Rac1^J11/Rac2^Δ

(Matusek et al., 2008)

DAAM^Ex68 has connective phenotype, enhanceable by Rac1^J11/Rac2^Δ

(Matusek et al., 2008)

DAAM^Ex68 has lateral longitudinal fascicle phenotype, enhanceable by Rac1^J11/Rac2^Δ

(Matusek et al., 2008)

DAAM^Ex68 has embryo phenotype, enhanceable by Rac1^J11/Rac2^Δ

(Matusek et al., 2008)

DAAM^Ex68, Rac1^J11 has neuropil phenotype, enhanceable by Rac2^Δ

(Matusek et al., 2008)

DAAM^Ex68, Rac1^J11 has fascicle phenotype, enhanceable by Rac2^Δ

(Matusek et al., 2008)

DAAM^Ex68, Rac1^J11 has connective phenotype, enhanceable by Rac2^Δ

(Matusek et al., 2008)

DAAM^Ex68 has connective phenotype, enhanceable by Rho1^72F

(Matusek et al., 2008)

DAAM^Ex68, Rac1^J11 has lateral longitudinal fascicle phenotype, enhanceable by Rac2^Δ

(Matusek et al., 2008)

DAAM^Ex68, Rac1^J11 has embryo phenotype, enhanceable by Rac2^Δ

(Matusek et al., 2008)

DAAM^Ex68 has neuropil phenotype, enhanceable by Rac1^J11/Rac2^Δ/Mtl^Δ

(Matusek et al., 2008)

DAAM^Ex68 has fascicle phenotype, enhanceable by Rac1^J11/Rac2^Δ/Mtl^Δ

(Matusek et al., 2008)

DAAM^Ex68 has connective phenotype, enhanceable by Rac1^J11/Rac2^Δ/Mtl^Δ

(Matusek et al., 2008)

DAAM^Ex68 has lateral longitudinal fascicle phenotype, enhanceable by Rac1^J11/Rac2^Δ/Mtl^Δ

(Matusek et al., 2008)

DAAM^Ex68 has embryo phenotype, enhanceable by Rac1^J11/Rac2^Δ/Mtl^Δ

(Matusek et al., 2008)

DAAM^Ex68, Rac1^J11, Rac2^Δ has neuropil phenotype, enhanceable by Mtl^Δ

(Matusek et al., 2008)

DAAM^Ex68 has lateral longitudinal fascicle phenotype, enhanceable by Rho1^72F

(Matusek et al., 2008)

DAAM^Ex68, Rac1^J11, Rac2^Δ has fascicle phenotype, enhanceable by Mtl^Δ

(Matusek et al., 2008)

DAAM^Ex68, Rac1^J11, Rac2^Δ has connective phenotype, enhanceable by Mtl^Δ

(Matusek et al., 2008)

DAAM^Ex68, Rac1^J11, Rac2^Δ has lateral longitudinal fascicle phenotype, enhanceable by Mtl^Δ

(Matusek et al., 2008)

DAAM^Ex68, Rac1^J11, Rac2^Δ has embryo phenotype, enhanceable by Mtl^Δ

(Matusek et al., 2008)

DAAM^Ex68 has neuropil phenotype, enhanceable by chic[+]/chic²²¹

(Matusek et al., 2008)

DAAM^Ex68 has fascicle phenotype, enhanceable by chic[+]/chic²²¹

(Matusek et al., 2008)

DAAM^Ex68 has connective phenotype, enhanceable by chic[+]/chic²²¹

(Matusek et al., 2008)

DAAM^Ex68 has lateral longitudinal fascicle phenotype, enhanceable by chic[+]/chic²²¹

(Matusek et al., 2008)

DAAM^Ex68 has embryo phenotype, enhanceable by Rho1^72F

(Matusek et al., 2008)

DAAM^Ex68 has embryo phenotype, enhanceable by chic[+]/chic²²¹

(Matusek et al., 2008)

DAAM^Ex68 has neuropil phenotype, enhanceable by ena[+]/ena^GC5

(Matusek et al., 2008)

DAAM^Ex68 has fascicle phenotype, enhanceable by ena[+]/ena^GC5

(Matusek et al., 2008)

DAAM^Ex68 has connective phenotype, enhanceable by ena[+]/ena^GC5

(Matusek et al., 2008)

DAAM^Ex68 has lateral longitudinal fascicle phenotype, enhanceable by ena[+]/ena^GC5

(Matusek et al., 2008)

DAAM^Ex68 has embryo phenotype, enhanceable by ena[+]/ena^GC5

(Matusek et al., 2008)

DAAM^Ex68 has neuropil phenotype, enhanceable by ena⁰²⁰²⁹/ena[+]

(Matusek et al., 2008)

DAAM^Ex68 has fascicle phenotype, enhanceable by ena⁰²⁰²⁹/ena[+]

(Matusek et al., 2008)

DAAM^Ex68 has connective phenotype, enhanceable by ena⁰²⁰²⁹/ena[+]

(Matusek et al., 2008)

DAAM^Ex68 has lateral longitudinal fascicle phenotype, enhanceable by ena⁰²⁰²⁹/ena[+]

(Matusek et al., 2008)

DAAM^Ex68 has embryo phenotype, enhanceable by ena⁰²⁰²⁹/ena[+]

(Matusek et al., 2008)

DAAM^Ex68 has neuropil phenotype, enhanceable by Rac1^J11

(Matusek et al., 2008)

DAAM^Ex68 has fascicle phenotype, enhanceable by Rac1^J11

(Matusek et al., 2008)

DAAM^Ex68 has connective phenotype, enhanceable by Rac1^J11

(Matusek et al., 2008)

DAAM^Ex68 has lateral longitudinal fascicle phenotype, enhanceable by Rac1^J11

(Matusek et al., 2008)

NOT Enhanced by

Statement

Reference

DAAM^Ex68 has NMJ bouton | larval stage phenotype, non-enhanceable by Ank2^XLΔ/Ank2^XLΔ

(Migh et al., 2018)

DAAM^Ex68 has NMJ bouton | larval stage phenotype, non-enhanceable by futsch^CR13

(Migh et al., 2018)

Suppressed by

Statement

Reference

DAAM^Ex68 has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible | partially by Ank2^XLΔ/Ank2^XLΔ

(Migh et al., 2018)

DAAM^Ex68 has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible | partially by Ank2^f00518/Ank2^f00518

(Migh et al., 2018)

DAAM^Ex1/DAAM^Ex68 has connective phenotype, suppressible | partially by Scer\GAL4^elav-C155/Mmus\Daam1^UAS.EGFP

(Matusek et al., 2008)

NOT suppressed by

Statement

Reference

DAAM^Ex68 has embryonic/larval neuromuscular junction | larval stage phenotype, non-suppressible by tkv⁷/tkv[+]

(Migh et al., 2018)

DAAM^Ex68 has NMJ bouton | larval stage phenotype, non-suppressible by tkv⁷/tkv[+]

(Migh et al., 2018)

DAAM^Ex68 has NMJ bouton | larval stage phenotype, non-suppressible by Ank2[+]/Ank2^f00518

(Migh et al., 2018)

DAAM^Ex68 has larval LL1 motor neuron | larval stage phenotype, non-suppressible by Ank2[+]/Ank2^f00518

(Migh et al., 2018)

DAAM^Ex68 has embryonic/larval neuromuscular junction | larval stage phenotype, non-suppressible by wg[+]/wg^l-8

(Migh et al., 2018)

DAAM^Ex68 has NMJ bouton | larval stage phenotype, non-suppressible by wg[+]/wg^l-8

(Migh et al., 2018)

DAAM^Ex68 has larval LL1 motor neuron | larval stage phenotype, non-suppressible by wg[+]/wg^l-8

(Migh et al., 2018)

DAAM^Ex68 has larval LL1 motor neuron | larval stage phenotype, non-suppressible by tkv⁷/tkv[+]

(Migh et al., 2018)

DAAM^Ex68 has embryonic/larval neuromuscular junction | larval stage phenotype, non-suppressible by dia[+]/dia⁵

(Migh et al., 2018)

DAAM^Ex68 has NMJ bouton | larval stage phenotype, non-suppressible by dia[+]/dia⁵

(Migh et al., 2018)

DAAM^Ex68 has larval LL1 motor neuron | larval stage phenotype, non-suppressible by dia[+]/dia⁵

(Migh et al., 2018)

DAAM^Ex68 has embryonic/larval neuromuscular junction | larval stage phenotype, non-suppressible by Lar[+]/Lar^13.2

(Migh et al., 2018)

DAAM^Ex68 has NMJ bouton | larval stage phenotype, non-suppressible by Lar[+]/Lar^13.2

(Migh et al., 2018)

DAAM^Ex68 has larval LL1 motor neuron | larval stage phenotype, non-suppressible by Lar[+]/Lar^13.2

(Migh et al., 2018)

DAAM^Ex68 has embryonic/larval neuromuscular junction | larval stage phenotype, non-suppressible by Ank2[+]/Ank2^f00518

(Migh et al., 2018)

Enhancer of

Statement

Reference

DAAM^Ex68 is an enhancer of embryonic/larval neuromuscular junction | larval stage phenotype of futsch^CR13

(Migh et al., 2018)

DAAM^Ex68 is an enhancer of NMJ bouton | larval stage phenotype of futsch^CR13

(Migh et al., 2018)

DAAM^Ex68 is an enhancer of larval LL1 motor neuron | larval stage phenotype of futsch^CR13

(Migh et al., 2018)

DAAM^Ex68/DAAM^Ex68 is an enhancer of embryonic/larval neuromuscular junction | larval stage phenotype of Ank2^f00518

(Migh et al., 2018)

DAAM^Ex68/DAAM^Ex68 is an enhancer of NMJ bouton | larval stage phenotype of Ank2^f00518

(Migh et al., 2018)

DAAM^Ex68/DAAM^Ex68 is an enhancer of embryonic/larval neuromuscular junction | larval stage phenotype of Ank2^XLΔ

(Migh et al., 2018)

DAAM^Ex68/DAAM^Ex68 is an enhancer of NMJ bouton | larval stage phenotype of Ank2^XLΔ

(Migh et al., 2018)

DAAM^Ex1/DAAM^Ex68 is an enhancer of filopodium phenotype of Arpc1¹

(Gonçalves-Pimentel et al., 2011)

Suppressor of

Statement

Reference

DAAM^Ex68/DAAM^Ex68 is a suppressor | partially of embryonic/larval neuromuscular junction | larval stage phenotype of Ank2^f00518

(Migh et al., 2018)

DAAM^Ex68/DAAM^Ex68 is a suppressor | partially of microtubule | larval stage phenotype of Ank2^f00518

(Migh et al., 2018)

DAAM^Ex68/DAAM^Ex68 is a suppressor | partially of embryonic/larval neuromuscular junction | larval stage phenotype of Ank2^XLΔ

(Migh et al., 2018)

DAAM^Ex68/DAAM^Ex68 is a suppressor | partially of microtubule | larval stage phenotype of Ank2^XLΔ

(Migh et al., 2018)

Other

Statement

Reference

DAAM^Ex68, futsch^CR13 has microtubule | larval stage phenotype

(Migh et al., 2018)

DAAM^Ex68, Frl^Ex83 has rhabdomere of eye photoreceptor cell | somatic clone | cell autonomous | adult stage phenotype

(Dollar et al., 2016)

Arp3^EP3640, DAAM[+]/DAAM^Ex68 has embryonic/larval central nervous system phenotype

(Gonçalves-Pimentel et al., 2011)

Arpc1^Q25sd, DAAM[+]/DAAM^Ex68 has embryonic/larval central nervous system phenotype

(Gonçalves-Pimentel et al., 2011)

Sop2[+]/Arpc1¹, DAAM^Ex68 has filopodium phenotype

(Gonçalves-Pimentel et al., 2011)

DAAM^Ex68, ena²³/ena[+] has filopodium phenotype

(Gonçalves-Pimentel et al., 2011)

Arpc1^Q25sd/Sop2[+], DAAM^Ex68 has embryonic/larval central nervous system phenotype

(Gonçalves-Pimentel et al., 2011)

Arp3^EP3640/Arp66B[+], DAAM^Ex68 has embryonic/larval central nervous system phenotype

(Gonçalves-Pimentel et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The larval muscle 4 neuromuscular junctions in either trio¹, DAAM^Ex68 double heterozygotes or dia⁵, DAAM^Ex68 double heterozygotes do not show significant changes in synaptic bouton number, as compared to controls.

The larval muscle 4 neuromuscular junctions in DAAM^Ex68, futsch^CR13 double mutants show fragmented presynaptic microtubules bundles, as compared to controls.

(Migh et al., 2018)

DAAM^Ex68/DAAM^Ex68; Frl^Ex83/Frl^Ex83 double mutant eye R-cell clones lack rhabdomere-associated pigment granules and double mutant R-cells show a partially penetrant cell autonomous small rhabdomere phenotype (compared to DAAM^Ex68/DAAM^Ex68 mutants).

(Dollar et al., 2016)

Cultured primary neurons derived from DAAM^Ex1/DAAM^Ex68 Sop2¹ embryos show a very strong reduction in the number of filopodia (to 5% of the wild type average) compared to control neurons. In live analyses, the mutant neurons show modestly increased protrusion rates and strongly increased retraction rates of filopodia.

Only 20% of cultured primary neurons derived from DAAM^Ex1/DAAM^Ex68 Sop2¹/Sop2^Q25sd embryos have neurites.

Cultured primary neurons derived from DAAM^Ex68/+ Sop2¹/+ and DAAM^Ex68/+ ena²³/+ double heterozygous embryos show a significant reduction in the number of filopodia compared to control neurons.

Cultured primary neurons derived from DAAM^Ex68/+ chic²²¹/+ double heterozygous embryos have a normal number of filopodia.

(Gonçalves-Pimentel et al., 2011)

A Rho1^72F heterozygous background weakly enhances the zygotic DAAM^Ex68 CNS phenotype.

A Rac1^J11 background enhances the zygotic DAAM^Ex68 CNS phenotype, with the severity of the phenotype correlating with the number of Rac1 copies removed. A DAAM^Ex68 heterozygous background strongly enhances the axonal growth defects exhibited by Rac1^J11 mutants.

A Rac1^J11/+; Rac2^Δ/+ background enhances both the zygotic DAAM^Ex68 and DAAM^Ex68; Rac1^J11/+ CNS phenotypes.

A Rac1^J11; Rac2^Δ, Mtl[Δ] heterozygous background enhances the zygotic DAAM^Ex68, DAAM^Ex68; Rac1^J11/+ and DAAM^Ex68; Rac1^J11/+; Rac2^Δ/+CNS phenotypes.

A chic²²¹ heterozygous background increases the proportion of DAAM^Ex68 mutant embryos that exhibit CNS defects.

A ena^GC5 heterozygous background increases the proportion of DAAM^Ex68 mutant embryos that exhibit CNS defects.

A ena⁰²⁰²⁹ heterozygous background increases the proportion of DAAM^Ex68 mutant embryos that exhibit CNS defects.

(Matusek et al., 2008)

Xenogenetic Interactions

Statement

Reference

Expression of Mmus\Daam1^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^elav-C155 rescues central nervous system axon defects in 70% of DAAM^Ex68/DAAM^Ex1 embryos derived from homozygous DAAM^Ex1 females.

(Matusek et al., 2008)

Complementation and Rescue Data

Fails to complement

DAAM^Ex36

(Matusek et al., 2006)

DAAM^Ex202

(Matusek et al., 2006)

DAAM^Ex65

(Matusek et al., 2006)

DAAM^Ex1

(Matusek et al., 2006)

DAAM^Ex184

(Matusek et al., 2006)

DAAM^Ex249

(Matusek et al., 2006)

Rescued by

DAAM^Ex68 is rescued by DAAM^UAS.cMb/Scer\GAL4^Toll-6-D42

(Migh et al., 2018)

DAAM^Ex68 is rescued by DAAM^{C.UAS.Tag:FLAG}/Scer\GAL4^Toll-6-D42

(Migh et al., 2018)

DAAM^Ex68 is rescued by DAAM^I732A.UAS/Scer\GAL4^Toll-6-D42

(Migh et al., 2018)

DAAM^Ex1/DAAM^Ex68 is rescued by Scer\GAL4^elav-C155/DAAM^UASp.cMa

(Matusek et al., 2008)

DAAM^Ex1/DAAM^Ex68 is rescued by DAAM^UASp.cMa/Scer\GAL4^Act5C.PI

(Matusek et al., 2008)

DAAM^Ex68 is rescued by DAAM^UASp.cMa/Scer\GAL4^Act.PU

(Matusek et al., 2006)

DAAM^Ex68 is rescued by DAAM^UASp.cMa/Scer\GAL4^Tub.PU

(Matusek et al., 2006)

Partially rescued by

DAAM^Ex68 is partially rescued by DAAM^UAS.cMb/Scer\GAL4^btl.PS

(Migh et al., 2018)

DAAM^Ex68 is partially rescued by DAAM^{C.UAS.Tag:FLAG}/Scer\GAL4^btl.PS

(Migh et al., 2018)

DAAM^Ex68 is partially rescued by DAAM^UASp.cMa/Scer\GAL4^Mef2.PU

(Molnár et al., 2014)

DAAM^Ex68 is partially rescued by Scer\GAL4^Mef2.PU/DAAM^UAS.PD

(Molnár et al., 2014)

DAAM^Ex68 is partially rescued by DAAM^UASp.cMa/Scer\GAL4^btl.PS

(Matusek et al., 2006)

Not rescued by

DAAM^Ex68 is not rescued by DAAM^876-881.UAS/Scer\GAL4^Toll-6-D42

(Migh et al., 2018)

DAAM^Ex68 is not rescued by DAAM^{I1042A.UAS.PD}/Scer\GAL4^Mef2.PU

(Molnár et al., 2014)

DAAM^Ex68 is not rescued by Scer\GAL4^Mef2.PU/DAAM^I732A.UAS.PB

(Molnár et al., 2014)

Comments

Expression of DAAM^{Scer\UAS.P\T.cMa} under the control of Scer\GAL4^Mef2.PU partially rescues the reduced motility and almost fully rescues the body and muscle size of late third instar DAAM^Ex68 larvae.

Expression of DAAM^Scer\UAS.PD under the control of Scer\GAL4^Mef2.PU almost fully rescues the reduced motility and the body and muscle size of late third instar DAAM^Ex68 larvae.

Expression of DAAM^{I1042A.Scer\UAS.PD} under the control of Scer\GAL4^Mef2.PU fails to rescue the reduced motility and the body and muscle size of late third instar DAAM^Ex68 larvae.

(Molnár et al., 2014)

The CNS defects revealed in DAAM^Ex68/DAAM^Ex1 mutants are fully rescued when DAAM^{Scer\UAS.P\T.cMa} is expressed under the control of the pan-neuronal Scer\GAL4^elav-C155 or the ubiquitous Scer\GAL4^Act5C.PI driver.

(Matusek et al., 2008)

Expression of DAAM^{Scer\UAS.P\T.cMa} under the control of Scer\GAL4^btl.PS rescues the tracheal phenotype of DAAM^Ex68 mutants.

(Matusek et al., 2006)

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Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

DAAM^EX68

(Migh et al., 2018)

DAAM^Ex68-null

(Gombos et al., 2015)

DAAM^Ex68

(Gazsó-Gerhát et al., 2023, Qu et al., 2017, Dollar et al., 2016, Gonçalves-Pimentel et al., 2011, Matusek et al., 2006)

DAAM^ex68

(Farkas et al., 2024)

Daam^Ex68

(Warner and Longmore, 2009)

dDAAM^Ex68

(Molnár et al., 2014, Matusek et al., 2008)

daam^ex68

(Pinheiro et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (13)

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